Mechanism that gap junctions determines size and distance of cell populations
Project/Area Number |
26291049
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Developmental biology
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Research Institution | Osaka University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
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Keywords | ギャップジャンクション / ゼブラフィッシュ / パターン形成 / 色素細胞 / 体表模様 / 骨形成 / 細胞間相互作用 / 電気生理解析 |
Outline of Final Research Achievements |
Gap junctions are intercellular channels that allow passage of ions and small molecules between adjacent cells. The zebrafish has been predicted to contain 39 connexin-encoding genes; the high number can be attributed to gene duplication during fish evolution, which resulted in diversified functions of gap junctions in teleosts. In this project we analyzed two connexin mutants, leopard and stoepsel, which are caused by the mutations in cx41.8 and cx43, respectively. In addition, we also analyzed cx39.4 which is involved in the skin pattern formation of zebrafish as cx41.8 is. Electrophysiological analysis revealed that Cx41.8 and Cx39.4 have different properties and function in a coordinated manner in skin pattern formation. Also electrophysiological analysis revealed that the growth-dependent malformation of the vertebrae in stp fish is likely caused by aberrant hemichannel activity of Connexin43 rather than by its reduced gap junction function due to the stp mutation
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Two Different Functions of Connexin43 Confer Two Different Bone Phenotypes in Zebrafish2016
Author(s)
Misu, A., Yamanaka, H., Aramaki, T., Kondo, S., Skerrett, I. M., Iovine, M. K. & Watanabe, M
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Journal Title
J Biol Chem
Volume: 291
Issue: 24
Pages: 12601-12611
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] The physiological characterization of Connexin41.8 and Connexin39.4, which are involved in the stripe pattern formation of zebrafish2016
Author(s)
Watanabe, M*., Sawada, R., Aramaki, T., Skerrett, I.M., & Kondo, S.
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Journal Title
J Biol Chem
Volume: 291
Issue: 3
Pages: 1053-1063
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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