Mechanisms of cell cycle regulation at G2/M and its diverse roles in plant development

• Project/Area Number: 26291058
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: ITO Masaki 名古屋大学, 生命農学研究科, 准教授 (10242851)
• Co-Investigator(Kenkyū-buntansha): 町田 泰則 名古屋大学, 理学(系)研究科(研究院), 教授 (80175596)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Takamasa 中部大学, 応用生物学部, 講師 (50535797) ; KUWATA Keiko 名古屋大学, トランスフォーマティブ生命分子研究所, 特任助教 (70624352) ; NAKAMICHI Norihito 名古屋大学, トランスフォーマティブ生命分子研究所, 特任助教 (90513440)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000); Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
• Keywords: 細胞周期 / 細胞増殖 / 転写制御 / タンパク質分解 / RNA代謝 / 環境ストレス / 細胞分裂 / 植物 / 核内倍加 / G2/M期制御 / 非対称分裂

Outline of Final Research Achievements

For controlling plant organ growth in changing environment, it is important to properly regulate proliferation of component cells in growing organs. It has been believed that regulation at G2/M in the cell cycle is particularly important for plants that show endoreplication in their organ growth. In our genetic studies using Arabidopsis mutant with deregulated activity of mitotic regulator APC/C that acts as E3 ubiquitin ligase, we showed the possibility that a novel mechanism of cell cycle checkpoint may exist and may inhibit G2/M progression when nuclear mRNA metabolism is occasionally impaired. We also showed that MYB3R transcription factors, known as central regulators of G2/M-specific genes, play an important role in growth inhibition under salt stress, partially through its roles in gibberellin signal transduction where MYB3Rs physically interact with DELLA proteins and mediate the growth inhibition caused by the action of DELLA.

Research Products

• [Int'l Joint Research] University of London/Aberystsyth University(英国)
• [Int'l Joint Research] University of Tubingen/University of Hamburg(ドイツ)
• [Int'l Joint Research] Biological Research Centre(ハンガリー)
• [Int'l Joint Research] University of California/Miami University(米国)
• [Int'l Joint Research] Polish Academy of Sciences(ポーランド)
• [Int'l Joint Research] University of London/Aberystwyth University(英国)
• [Int'l Joint Research] University of Tubingen(ドイツ)
• [Int'l Joint Research] Biological Research Centre(ハンガリー)
• [Int'l Joint Research] Univ. Natural Resources Life Sci(オーストリア)
• [Journal Article] Autocrine regulation of stomatal differentiation potential by EPF1 and ERECTALIKE1 ligand-receptor signaling. (2017)
  • Author(s): Qi, X., Han, S.K., Dang, J.H., Garrick, J.M., Ito, M., Hofstetter, A.K., Torii, K.U.
  • Journal Title: Elife Volume: 6 Pages: 24012-24012
  • DOI: 10.7554/elife.24102
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Haspin kinase has multiple functions in the plant cell division regulatory network. (2016)
  • Author(s): Kozgunova, E., Suzuki, T., Ito, M., Higashiyama, T., Kurihara, D.
  • Journal Title: Plant Cell Physiol. Volume: 57 Issue: 4 Pages: 848-861
  • DOI: 10.1093/pcp/pcw030
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Unique mechanisms of plant cell division regulated by multi-protein complexes (2016)
  • Author(s): 伊藤正樹, 笹部美知子, 町田泰則
  • Journal Title: Leading Author's Volume: 5 Pages: e005
  • DOI: 10.7875/leading.author.5.e005
  • Peer Reviewed / Open Access
• [Journal Article] MYB3Rs, plant homologs of Myb oncoproteins, control cell cycle-regulated transcription and form DREAM-like complexes. (2015)
  • Author(s): Kobayashi, K., Suzuki, T., Iwata, E., Magyar, Z., Bogre, L., Ito, M.
  • Journal Title: Transcription Volume: 6 Issue: 5 Pages: 106-111
  • DOI: 10.1080/21541264.2015.1109746
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Transcriptional repression by MYB3R proteins regulates plant organ growth. (2015)
  • Author(s): Kobayashi K, Suzuki T, Iwata E, Nakamichi N, Suzuki T, Chen P, Ohtani M, Ishida T, Hosoya H, Müller S, Leviczky T, Pettkó-Szandtner A, Darula Z, Iwamoto A, Nomoto M, Tada Y, Higashiyama T, Demura T, Doonan JH, Hauser MT, Sugimoto K, Umeda M, Magyar Z, Bögre L, Ito M
  • Journal Title: EMBO Journal Volume: 34 Issue: 15 Pages: 1992-2007
  • DOI: 10.15252/embj.201490899
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Reprogramming of plant cells induced by 6b oncoproteins from the plant pathogen Agrobacterium. (2015)
  • Author(s): Ito, M., Machida, Y.
  • Journal Title: J. Plant Res. Volume: 未定 Issue: 3 Pages: 423-435
  • DOI: 10.1007/s10265-014-0694-3
  • Peer Reviewed / Open Access
• [Journal Article] Rapid Elimination of the Persistent Synergid through a Cell Fusion Mechanism. (2015)
  • Author(s): 丸山大輔、Ronny Völz、竹内英憲、森稔幸、井川智子、栗原大輔、河島友和、植田美那子、伊藤正樹、梅田正明、西川周一、Rita Groß-Hardt、東山哲也
  • Journal Title: Cell Volume: 未定 Issue: 4 Pages: 907-918
  • DOI: 10.1016/j.cell.2015.03.018
  • Peer Reviewed / Open Access
• [Journal Article] Genetic interaction between G2/M phase-specific transcription factor MYB3R4 and MAPKKK ANP3 for execution of cytokinesis in Arabidopsis thaliana. (2015)
  • Author(s): Saito, T., Fujikawa, H., Haga, N., Suzuki, T., Machida, Y., Ito, M.
  • Journal Title: Plant Signal. Behav. Volume: 未定 Issue: 3 Pages: e990817-e990817
  • DOI: 10.4161/15592324.2014.990817
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Rapid elimination of the persistent synergid through a cell-fusion for polytubey block. (2015)
  • Author(s): Maruyama, D., Völz, R., Takeuchi, H., Mori, T., Igawa, T., Kurihara, D., Kawashima, T., Ueda, M., Ito, M., Umeda, M., Nishikawa, S., Groß-Hardt, R., Higashiyama, T.
  • Journal Title: Cell Volume: 未定
  • Peer Reviewed
• [Journal Article] The carboxyl-terminal tail of the stalk of Arabidopsis NACK1/HINKEL kinesin is required for its localization to the cell plate formation site (2015)
  • Author(s): Sasabe, M., Ishibashi, N., Haruta, T., Minami, A., Kurihara, D., Higashiyama, T., Nishihama, R., Ito, M., and Machida, Y.
  • Journal Title: J. Plant Res. Volume: 128 Issue: 2 Pages: 327-336
  • DOI: 10.1007/s10265-014-0687-2
  • Peer Reviewed / Open Access
• [Presentation] 核内倍加を起こさないイネにおける細胞増殖制御の特性 (2017)
  • Author(s): 栂根美佳、坂本勇貴、長谷川淳子、松永幸大、伊藤正樹
  • Organizer: 第58会日本植物生理学会年会
  • Place of Presentation: 鹿児島
  • Year and Date: 2017-03-18
• [Presentation] RNA代謝異常と細胞周期抑制を結ぶ新奇チェックポイント機構の存在の可能性 (2017)
  • Author(s): 高瀬めぐみ、鈴木孝征、大谷美沙都、伊藤正樹
  • Organizer: 第58会日本植物生理学会年会
  • Place of Presentation: 鹿児島
  • Year and Date: 2017-03-18
• [Presentation] Transcriptional repression by MYB3R proteins is required for spatial and temporal regulation of cell division. (2016)
  • Author(s): Kobayashi, K., Suzuki, T., Magyar, Z., Bogre, L., Ito, M
  • Organizer: The 2016 Cold Spring Harbor Asia Conference “Latest advances in plant development & environmental response”
  • Place of Presentation: Awaji
  • Year and Date: 2016-12-01
  • Int'l Joint Research
• [Presentation] なぜイネは核内倍加を起こさないのか (2016)
  • Author(s): 栂根美佳、長谷川淳子、松永幸大、伊藤正樹
  • Organizer: 日本植物学会第80会大会
  • Place of Presentation: 宜野湾
  • Year and Date: 2016-09-16
• [Presentation] サイクリン依存性キナーゼ阻害因子SMRの発現制御に関わるGRASファミリー転写因子 (2016)
  • Author(s): 鈴木俊哉、伊藤正樹
  • Organizer: 日本植物学会第80会大会
  • Place of Presentation: 宜野湾
  • Year and Date: 2016-09-16
• [Presentation] G2/M期制御に関わるMYB転写因子のジベレリンシグナル伝達系における役割 (2016)
  • Author(s): 奥村徹、伊藤正樹
  • Organizer: 日本植物学会第80会大会
  • Place of Presentation: 宜野湾
  • Year and Date: 2016-09-16
• [Presentation] 塩ストレス下での成長抑制に必須なシロイヌナスナMYB3R転写抑制因子 (2016)
  • Author(s): 奥村徹、鈴木孝征、東山哲也、伊藤正樹
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡
  • Year and Date: 2016-03-20
• [Presentation] シロイヌナズナを用いた後期促進複合体APC/Cの新奇標的因子の同定 (2016)
  • Author(s): 鈴木俊哉、桑田啓子、伊藤正樹
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡
  • Year and Date: 2016-03-20
• [Presentation] 多倍数体細胞を生じるイネ変異体の解析 (2015)
  • Author(s): 栂根美佳、栂根一夫、寺内良平、鈴木 孝征、東山哲也、長岐清孝、伊藤正樹
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-03
• [Presentation] 植物特異的なGRASファミリー転写因子による細胞分裂とDNA倍数性の制御 (2015)
  • Author(s): 鈴木俊哉、Christian Breuer、石田喬志、鈴木孝征、東山哲也、杉本慶子、伊藤正樹
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-02
• [Presentation] 葯壁タペート細胞で起こるDNA倍加のメカニズム (2015)
  • Author(s): 鈴木俊哉、浜村有希、東山哲也、石黒澄衛、伊藤正樹
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-07
• [Presentation] イネ体細胞のDNA倍数性に影響を与える遺伝子の解析 (2015)
  • Author(s): 栂根美佳、井上慎子、栂根一夫、寺内良平、永澤信洋、前川雅彦、伊藤正樹
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-18
• [Presentation] GRASファミリー転写因子によるシロイヌナズナの細胞分裂と核内倍加の制御 (2015)
  • Author(s): 鈴木俊哉，Christian Breuer，石田喬志，鈴木孝征，東山哲也，杉本慶子，伊藤正樹
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-18
• [Presentation] イネ体細胞のDNA倍数性に影響を与える遺伝子の解析 (2014)
  • Author(s): 栂根美佳、井上慎子、栂根一夫、寺内良平、永澤信洋、前川雅彦、伊藤正樹
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25
• [Presentation] 植物特異的なGRASファミリー転写因子による細胞周期の制御 (2014)
  • Author(s): 鈴木俊哉，Christian Breuer，石田喬志，鈴木孝征，東山哲也，杉本慶子，伊藤正樹
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25
• [Remarks] 伊藤研究グループ
  • URL: https://www.agr.nagoya-u.ac.jp/~bunka/ito_title%20page.html
• [Remarks] 伊藤研究グループ
  • URL: https://www.agr.nagoya-u.ac.jp/̃bunka/ito_title%20page.html
• [Patent(Industrial Property Rights)] 植物のDNA倍加誘導法 (2016)
  • Inventor(s): 伊藤正樹、栂根美佳、梅田正明
  • Industrial Property Rights Holder: 伊藤正樹、栂根美佳、梅田正明
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-143238
  • Filing Date: 2016-07-21