Studies on the molecular mechanisms underlying the transition from cell division to cell differentiation

• Project/Area Number: 26291061
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: UMEDA Masaaki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (80221810)
• Co-Investigator(Kenkyū-buntansha): 高橋 直紀 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (40553623)
• Co-Investigator(Renkei-kenkyūsha): SEKI Motoaki 理化学研究所, 環境資源科学研究センター, チームリーダー (80281624) ; FUKAO Yoichiro 立命館大学, 生命科学部, 准教授 (80432590)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000); Fiscal Year 2016: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 発生・分化 / 植物ホルモン / 細胞分裂 / DNA倍加 / クロマチン / 発現制御 / ゲノム / 植物 / シグナル伝達

Outline of Final Research Achievements

In this study, we revealed the mechanisms underlying the transition from cell division to cell differentiation by conducting functional analyses of KRPs, which are involved in degradation of B-type response regulators, and by analyzing the epigenetic regulation in triggering DNA polyploidization in roots. We found that some KRPs are expressed in the transition zone in roots and play an important role in an early onset of cell differentiation in response to DNA damage. Our results also indicated the possibility that auxin controls chromatin structure, thereby being associated with the transition from cell division to cell differentiation.

Research Products

• [Journal Article] Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote (2017)
  • Author(s): Ueda M, Aichinger E, Gong W, Groot E, Verstraeten I, Vu LD, De Smet I, Higashiyama T, Umeda M, Laux T
  • Journal Title: Genes & Development Volume: 31 Issue: 6 Pages: 617-627
  • DOI: 10.1101/gad.292409.116
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] DNA damage inhibits lateral root formation by upregulating cytokinin biosynthesis genes in Arabidopsis thaliana. (2016)
  • Author(s): Davis, M.M., Ogita, N., Inagaki, S., Takahashi, N., Umeda, M.
  • Journal Title: Genes Cells Volume: 21 Issue: 11 Pages: 1195-1208
  • DOI: 10.1111/gtc.12436
  • NAID: 120006537391
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis. (2016)
  • Author(s): Weimer, A.K., Biedermann, S., Harashima, H., Roodbarkelari, F., Takahashi, N., Foreman, J., Guan, Y., Pochon, G., Heese, M., Van Damme, D., Sugimoto, K., Koncz, C., Doerner, P., Umeda, M., Schnittger, A.
  • Journal Title: EMBO J Volume: 35 Issue: 19 Pages: 2068-2086
  • DOI: 10.15252/embj.201593083
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Combination of synthetic chemistry and live-cell imaging identified a rapid cell division inhibitor in tobacco and Arabidopsis thaliana (2016)
  • Author(s): Nambo, M., Kurihara, D., Yamada, T., Nishiwaki-Ohkawa, T., Kadofusa, N., Kimata, Y., Kuwata, K., Umeda, M. and Ueda, M.
  • Journal Title: Plant Cell Physiol. Volume: 57 Issue: 11 Pages: 2255-2268
  • DOI: 10.1093/pcp/pcw140
  • NAID: 120006220249
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cytrap marker systems for in vivo visualization of cell cycle progression in Arabidopsis (2016)
  • Author(s): Sugamata Aki, S. and Umeda, M.
  • Journal Title: Methods Mol. Biol. Volume: 1370 Pages: 51-57
  • DOI: 10.1007/978-1-4939-3142-2_4
  • ISBN: 9781493931415, 9781493931422
• [Journal Article] Cyclin-dependent kinase-activating kinases CDKD;1 and CDKD;3 are essential for preserving mitotic activity in Arabidopsis thaliana (2015)
  • Author(s): Takatsuka, H., Umeda-Hara, C. and Umeda, M.
  • Journal Title: Plant J. Volume: 82 Issue: 6 Pages: 1004-1017
  • DOI: 10.1111/tpj.12872
  • NAID: 120006537393
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] DNA double-strand breaks induce the expression of flavin-containing monooxygenase and reduce root meristem size in Arabidopsis thaliana (2015)
  • Author(s): Chen, P. and Umeda, M.
  • Journal Title: Genes Cells Volume: 20 Issue: 8 Pages: 636-646
  • DOI: 10.1111/gtc.12255
  • NAID: 120006537392
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Transcriptional repression by MYB3R proteins regulates plant organ growth. (2015)
  • Author(s): Kobayashi K, Suzuki T, Iwata E, Nakamichi N, Suzuki T, Chen P, Ohtani M, Ishida T, Hosoya H, Müller S, Leviczky T, Pettkó-Szandtner A, Darula Z, Iwamoto A, Nomoto M, Tada Y, Higashiyama T, Demura T, Doonan JH, Hauser MT, Sugimoto K, Umeda M, Magyar Z, Bögre L, Ito M
  • Journal Title: EMBO Journal Volume: 34 Issue: 15 Pages: 1992-2007
  • DOI: 10.15252/embj.201490899
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Epigenetic control of cell division and cell differentiation in the root apex (2015)
  • Author(s): Takatsuka, H. and Umeda, M.
  • Journal Title: Front. Plant Sci. Volume: 6 Pages: 1178-1178
  • DOI: 10.3389/fpls.2015.01178
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Hormonal control of cell division and elongation along differentiation trajectories in roots (2014)
  • Author(s): Takatsuka, H. and Umeda, M.
  • Journal Title: J. Exp. Bot. Volume: 65 Issue: 10 Pages: 2633-2643
  • DOI: 10.1093/jxb/ert485
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cytokinins promote onset of endoreplication by controlling cell cycle machinery (2014)
  • Author(s): Takahashi, N. and Umeda, M.
  • Journal Title: Plant Signal. Behav. Volume: 9 Issue: 8 Pages: e29396-e29396
  • DOI: 10.4161/psb.29396
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A dual-color marker system for in vivo visualization of cell cycle progression in Arabidopsis (2014)
  • Author(s): Yin, K., Ueda, M., Takagi, H., Kajihara, T., Sugamata Aki, S., Nobusawa, T., Umeda-Hara, M. Umeda
  • Journal Title: Plant J. Volume: 80 Issue: 3 Pages: 541-552
  • DOI: 10.1111/tpj.12652
  • NAID: 120006537394
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Control of the cell cycle in two distinct cell files of the root epidermis (2017)
  • Author(s): 杉山輝樹、高塚大知、梅田正明
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大（鹿児島県鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] Roles of epigenetic regulation in inducing endoreplication in plants (2017)
  • Author(s): 高塚大知、梅田正明
  • Organizer: Plant Organ Growth Symposium 2017
  • Place of Presentation: Centro de Congresos, Elche, Spain
  • Year and Date: 2017-03-15
  • Int'l Joint Research
• [Presentation] Hormonal control of genome integrity in roots (2017)
  • Author(s): 梅田正明、高橋直紀
  • Organizer: Plant Organ Growth Symposium 2017
  • Place of Presentation: Centro de Congresos, Elche, Spain
  • Year and Date: 2017-03-15
  • Int'l Joint Research / Invited
• [Presentation] Control of the cell cycle in two distinct cell files of the root epidermis (2016)
  • Author(s): 杉山輝樹、高塚大知、梅田正明
  • Organizer: International Conference on Arabidopsis Research
  • Place of Presentation: Gyeongju Hwabaek International Convention Center, Gyeongju, Korea
  • Year and Date: 2016-06-29
  • Int'l Joint Research
• [Presentation] Roles of auxin in controlling chromatin structure (2016)
  • Author(s): Aida Nazlyn Nazari、高塚大知、梅田正明
  • Organizer: International Conference on Arabidopsis Research
  • Place of Presentation: Gyeongju Hwabaek International Convention Center, Gyeongju, Korea
  • Year and Date: 2016-06-29
  • Int'l Joint Research
• [Presentation] Chromatin structure controls the competence of endoreplication (2016)
  • Author(s): 高塚大知、梅田正明
  • Organizer: International Conference on Arabidopsis Research
  • Place of Presentation: Gyeongju Hwabaek International Convention Center, Gyeongju, Korea
  • Year and Date: 2016-06-29
  • Int'l Joint Research
• [Presentation] Hormonal control of genome integrity in the root meristem (2016)
  • Author(s): 梅田正明
  • Organizer: International Conference on Arabidopsis Research
  • Place of Presentation: Gyeongju Hwabaek International Convention Center, Gyeongju, Korea
  • Year and Date: 2016-06-29
  • Int'l Joint Research / Invited
• [Presentation] 根におけるB-type ARRの空間的発現を制御するKISS ME DEADLY遺伝子の発現解析 (2016)
  • Author(s): 野口博史、高塚大知、梅田正明
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] Roles of auxin in controlling chromatin structure (2016)
  • Author(s): Aida Nazlyn Nazari、高塚大知、梅田正明
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] Control of the cell cycle in two distinct cell files of the root epidermis (2016)
  • Author(s): 杉山輝樹、高塚大知、梅田正明
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] Maintenance of genome integrity in root stem cells under DNA stress (2016)
  • Author(s): 高橋直紀、藤本啓介、梅田正明
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大（岩手県盛岡市）
  • Year and Date: 2016-03-18
  • Invited
• [Presentation] Roles of epigenetic regulation in inducing DNA polyploidization in plants (2016)
  • Author(s): 高塚大知、梅田正明
  • Organizer: Keystone Symposia "Plant Epigenetics: From Genotype to Phenotype"
  • Place of Presentation: Sagebrush Inn & Suites, Taos, New Mexico, USA
  • Year and Date: 2016-02-15
  • Int'l Joint Research
• [Presentation] 根端分裂組織のサイズ制御機構 (2015)
  • Author(s): 高橋直紀、丸池加奈子、高塚大知、梅田正明
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ・新潟コンベンションセンター（新潟県新潟市中央区）
  • Year and Date: 2015-09-06
  • Invited
• [Presentation] 極性をもった根の細胞成長の制御メカニズム (2015)
  • Author(s): 高塚大知、梅田正明
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ・新潟コンベンションセンター（新潟県新潟市中央区）
  • Year and Date: 2015-09-06
  • Invited
• [Presentation] Epidermal cells elongate with polarity in Arabidopsis roots (2015)
  • Author(s): 高塚大知、梅田正明
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大（東京都世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Cytokinins promote rapid cell elongation in Arabidopsis roots (2014)
  • Author(s): 高塚大知、梅田正明
  • Organizer: Auxins and Cytokinins in Plant Development International Symposium 2014
  • Place of Presentation: プラハ（チェコ）
  • Year and Date: 2014-06-29 – 2014-07-04
• [Remarks] 植物成長制御研究室ホームページ
  • URL: http://bsw3.naist.jp/umeda/
• [Remarks] 植物成長制御研究室ホームページ
  • URL: http://bsw3.naist.jp/umeda/index.html