| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
Following immune activation, defense-related genes become sensitized to second elicitation throughout the plant, termed defense priming. We show that transcription-permissive histone H3 tri-methylation at the 4th Lysine (H3K4me3) and transcription-repressive H3K27me3 both contribute to defense priming in Arabidopsis thaliana. Our RNA-seq analysis has revealed a genome-wide inventory of priming target genes, including those dependent on PRC2 that mediates H3K27me3. We have obtained and been currently analyzing chromatin immunoprecipitation (ChIP)-seq profiles with H3K4me3- and H3K27me3-specific antibodies. This is expected to reveal genome-wide dynamics of these histone modifications during and after the establishment of defense priming. Together, our studies have for the first time revealed a critical role for both H3K4me3 and H3K27me3, together with their target genes and modification dynamics, in defense priming of plants.
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