Histone modification-mediated control of defense-related gene expression in plant immunity
Project/Area Number |
26291062
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Nara Institute of Science and Technology |
Principal Investigator |
Saijo Yusuke 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (50587764)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
|
Keywords | 遺伝子発現制御 / ヒストン修飾 / エピジェネティクス / 植物免疫 / 遺伝子発現 / 植物微生物相互作用 / 植物分子機能 / ゲノム / 植物 / 遺伝子 / 発現制御 / 微生物 / 環境 / プライミング / クロマチン |
Outline of Final Research Achievements |
Following immune activation, defense-related genes become sensitized to second elicitation throughout the plant, termed defense priming. We show that transcription-permissive histone H3 tri-methylation at the 4th Lysine (H3K4me3) and transcription-repressive H3K27me3 both contribute to defense priming in Arabidopsis thaliana. Our RNA-seq analysis has revealed a genome-wide inventory of priming target genes, including those dependent on PRC2 that mediates H3K27me3. We have obtained and been currently analyzing chromatin immunoprecipitation (ChIP)-seq profiles with H3K4me3- and H3K27me3-specific antibodies. This is expected to reveal genome-wide dynamics of these histone modifications during and after the establishment of defense priming. Together, our studies have for the first time revealed a critical role for both H3K4me3 and H3K27me3, together with their target genes and modification dynamics, in defense priming of plants.
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Report
(4 results)
Research Products
(20 results)