Study on Fe-S cluster assembly genes in Arabidopsis thaliana

• Project/Area Number: 26291063
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Yokohama City University
• Principal Investigator: Kinoshita Tetsu 横浜市立大学, 木原生物学研究所, 教授 (60342630)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000); Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: エピジェネティクス / Fe-S cluster assembly / インプリンティング / DNA脱メチル化 / シロイヌナズナ

Outline of Final Research Achievements

DNA demethylation plays important roles for many aspects of plant development and gene expression control related to environmental adaptation. In this study, we identified the cytosolic iron-sulfur cluster assembly (CIA) genes that are required for activation and DNA demethylation of the maternally expressed imprinted genes in the central cell of the femalegametophyte in A. thaliana. Our detailed analysis using one of the mutation in the CIA components revealed that this pathway also controls genes for plant immunity and stress tolerance in vegetative tissues.

Research Products

• [Journal Article] Epigenetic role for the conserved Fe-S cluster biogenesis protein AtDRE2 in Arabidopsis thaliana (2014)
  • Author(s): Buzas, D., Nakamura, M., Kinoshita, T.
  • Journal Title: PNAS Volume: 111 Issue: 37 Pages: 13565-13570
  • DOI: 10.1073/pnas.1404058111
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Epigeenetic Controls and reproductive barriers in Endosperm (2016)
  • Author(s): Tetsu Kinoshita
  • Organizer: The 24th International Congress on Plant Reproduction
  • Place of Presentation: Tucson
  • Year and Date: 2016-03-21
  • Int'l Joint Research / Invited
• [Presentation] シロイヌナズナにおけるDNA脱メチル化機構 (2014)
  • Author(s): 木下 哲
  • Organizer: 日本遺伝学会
  • Place of Presentation: 長浜バイオ大学
  • Year and Date: 2014-09-17
  • Invited
• [Remarks]
  • URL: http://www.yokohama-cu.ac.jp/univ/pr/press/140902_res.html