Study on physiological significance and mechanisms of a novel hypothalamic secretory protein
| Project/Area Number |
26291066
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
岩越 栄子 広島大学, 総合科学研究科, 研究員 (50311296)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | 視床下部 / 神経ペプチド / ペプチド合成 / 摂食行動 / 脂肪蓄積 / 小タンパク質 / 神経投射 / 分泌タンパク質 / 分泌小タンパク質 / 成長 |
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| Outline of Final Research Achievements |
We recently identified a novel cDNA encoding a small secretory protein of 80 amino acid residues, termed neurosecretory protein GL (NPGL), from the chicken hypothalamus. Homologs of NPGL have been reported to be present in mammals, such as rat and mouse. In this study, we optimized the synthesis of NPGL by microwave-assisted solid-phase peptide synthesis. We found that NPGL increased food intake and the mass of white adipose tissue (WAT) in rats. NPGL induced carbohydrate intake. NPGL mRNA expression was upregulated by fasting and low insulin levels. Subsequently, we identified the cDNA encoding NPGL from the mouse hypothalamus. We found that NPGL mRNA is robustly expressed in the lateroposterior part of the arcuate nucleus in the hypothalamus. NPGL-immunoreactive fibers were observed in close anatomical contact with a-MSH neurons in the arcuate nucleus. NPGL mRNA expression was elevated by fasting and reduced by feeding of a high-fat diet. Furthermore, NPGL increased food intake.
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Report
(5 results)
Research Products
(31 results)
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[Journal Article] Neurosecretory protein GL, a hypothalamic small secretory protein, participates in energy homeostasis in male mice2017
Author(s)
Matsuura D＊, Shikano K＊, Saito T＊, Iwakoshi-Ukena E, Furumitsu M, Ochi Y, Sato M, Bentley GE, Kriegsfeld LJ, Ukena K
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Journal Title
Endocrinology
Volume: 158
Issue: 5
Pages: 1120-1129
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Neurosecretory protein GL stimulates food intake, de novo lipogenesis, and onset of obesity.2017
Author(s)
Iwakoshi-Ukena E, Shikano K, Kondo K, Taniuchi S, Furumitsu M, Ochi Y, Sasaki T, Okamoto S, Bentley GE, Kriegsfeld LJ, Minokoshi Y, Ukena K.
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Identification of a cDNA encoding a novel small secretory protein, neurosecretory protein GL, in the chicken hypothalamic infundibulum.2014
Author(s)
Ukena K, Iwakoshi-Ukena E, Taniuchi S, Bessho Y, Maejima S, Masuda K, Shikano K, Kondo K, Furumitsu M, Tachibana T.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 446
Issue: 1
Pages: 298-303
DOI
Related Report
Peer Reviewed / Open Access
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