| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
We recently identified a novel cDNA encoding a small secretory protein of 80 amino acid residues, termed neurosecretory protein GL (NPGL), from the chicken hypothalamus. Homologs of NPGL have been reported to be present in mammals, such as rat and mouse. In this study, we optimized the synthesis of NPGL by microwave-assisted solid-phase peptide synthesis. We found that NPGL increased food intake and the mass of white adipose tissue (WAT) in rats. NPGL induced carbohydrate intake. NPGL mRNA expression was upregulated by fasting and low insulin levels. Subsequently, we identified the cDNA encoding NPGL from the mouse hypothalamus. We found that NPGL mRNA is robustly expressed in the lateroposterior part of the arcuate nucleus in the hypothalamus. NPGL-immunoreactive fibers were observed in close anatomical contact with a-MSH neurons in the arcuate nucleus. NPGL mRNA expression was elevated by fasting and reduced by feeding of a high-fat diet. Furthermore, NPGL increased food intake.
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