Molecular mechanisms underlying heterochromatin assembly

• Project/Area Number: 26291072
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Genetics/Chromosome dynamics
• Research Institution: National Institute for Basic Biology (2016-2017); Nagoya City University (2014-2015)
• Principal Investigator: Nakayama Jun-ichi 基礎生物学研究所, クロマチン制御研究部門, 教授 (60373338)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000); Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
• Keywords: 遺伝子 / 発現制御 / ヘテロクロマチン / 分裂酵母 / ユビキチン化 / ユビキチン

Outline of Final Research Achievements

In this study, we focused on Clr4 methyltransferase complex (CLRC), and investigated a functional link between ubiquitin modifications and heterochromatin assembly. Affinity purified CLRC was shown to ubiquitylate histone H3. We further demonstrated that H3 ubiquitylation was tightly associated with H3K9me-enriched chromatin and that H3 ubiquitylation promotes H3K9 methylation by Clr4.

Research Products

• [Journal Article] Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly (2017)
  • Author(s): Atsuko Shirai, Takayuki Kawaguchi, Hideaki Shimojo, Daisuke Muramatsu, Mayumi Ishida-Yonetani, Yoshifumi Nishimura, Hiroshi Kimura, Jun-ichi Nakayama, Yoichi Shinkai
  • Journal Title: eLIFE Volume: 6
  • DOI: 10.7554/elife.25317
  • Peer Reviewed / Open Access
• [Journal Article] The intron in centromeric noncoding RNA facilitates RNAi-mediated formation of heterochromatin. (2017)
  • Author(s): Mutazono, M., Morita, M., Tsukahara, C., Chinen, M., Nishioka, S., Yumikake, T., Dohke, K., Sakamoto, M., Ideue, T., Wakayama, J.I., Ishii, K., Tani, T.
  • Journal Title: PLoS Genetics Volume: 13 Issue: 2 Pages: e1006606-e1006606
  • DOI: 10.1371/journal.pgen.1006606
  • NAID: 120006697213
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly (2017)
  • Author(s): Jun-ichi Nakayama
  • Organizer: 12th Asian Epigenomics Meeting 2017
  • Int'l Joint Research / Invited
• [Presentation] 高次クロマチン構造形成におけるヒストン修飾のクロストーク (2016)
  • Author(s): 大屋恵梨子，田中万葉，西淵剛平，中川れい子，町田晋一，胡桃坂仁志，田上英明，中山 潤一
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-12-02
  • Invited
• [Presentation] 分裂酵母におけるヘテロクロマチン構造形成の分子機構 (2016)
  • Author(s): 中山潤一
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 東北大学（宮城県仙台市）
  • Year and Date: 2016-09-27
  • Invited
• [Presentation] 分裂酵母のヘテロクロマチン形成におけるCLRC複合体の役割 (2016)
  • Author(s): 大屋恵梨子，田中万葉，西淵剛平，中川れい子，町田晋一，胡桃坂仁志，田上英明，中山 潤一
  • Organizer: 酵母遺伝学フォーラム第49回研究報告会
  • Place of Presentation: 舞子ビラ（兵庫県神戸市垂水区）
  • Year and Date: 2016-09-11
• [Presentation] Crosstalk between histone modifications during heterochromatin assembly (2016)
  • Author(s): Jun-ichi Nakayama
  • Organizer: Kick-Off Symposium in Nagoya City University 2016 "Regulatory Mechanisms of Epigenetic Information and Their Clinical Applications"
  • Place of Presentation: 名古屋市立大学（名古屋市）
  • Year and Date: 2016-02-29
  • Int'l Joint Research / Invited
• [Presentation] ヒストンのメチル化修飾を維持する分子機構 (2016)
  • Author(s): 中山潤一
  • Organizer: 第3回ヒストンバリアント研究会
  • Place of Presentation: 早稲田大学（新宿区）
  • Year and Date: 2016-02-28
  • Invited
• [Presentation] ヘテロクロマチン構造形成におけるCLRC複合体の役割 (2016)
  • Author(s): 大屋恵梨子、西淵剛平、中川れい子、田中万葉、町田晋一、胡桃坂仁志、田上英明、中山潤一
  • Organizer: 第33回染色体ワークショップ、第14回核ダイナミクス研究会
  • Place of Presentation: 松島一の坊（宮城郡松島町）
  • Year and Date: 2016-01-13
• [Presentation] Crosstalk between histone modifications during heterochromatin assembly (2015)
  • Author(s): Jun-ichi Nakayama
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド（神戸市）
  • Year and Date: 2015-12-04
  • Invited
• [Presentation] Crosstalk between histone modifications during heterochromatin assembly (2015)
  • Author(s): Jun-ichi Nakayama
  • Organizer: Forefront of Chromosome Biology
  • Place of Presentation: 京都大学（京都市）
  • Year and Date: 2015-08-10
  • Int'l Joint Research / Invited
• [Presentation] Crosstalk between histone modifications during heterochromatin assembly (2015)
  • Author(s): Eriki Oya, Mayo Tanaka, Gohei Nishibuchi, Reiko Nakagawa, Shinichi Machida, Hitoshi Kurumizaka, Hedeaki Tagami, Jun-ichi Nakayama
  • Organizer: The 8th International Fission Yeast Meeting
  • Place of Presentation: 生田神社（神戸市）
  • Year and Date: 2015-06-22
  • Int'l Joint Research / Invited
• [Presentation] 高次クロマチン構造の形成と維持の分子機構 (2015)
  • Author(s): 中山潤一
  • Organizer: 2014年度 遺伝研究会「単細胞の増殖メカニズムの先端的研究」
  • Place of Presentation: 国立遺伝学研究所（三島市）
  • Year and Date: 2015-03-24
  • Invited
• [Presentation] 分裂酵母における高次クロマチン構造の形成と維持の分子機構 (2014)
  • Author(s): 中山潤一
  • Organizer: 2014年度 遺伝研研究会「クロマチンによるゲノムDNAの機能発現メカニズム」
  • Place of Presentation: 国立遺伝学研究所（三島市）
  • Year and Date: 2014-10-30
  • Invited