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Elucidation of the establishment mechanism of the secondary symbiosis and the artificial induction

Research Project

Project/Area Number 26291073
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Evolutionary biology
Research InstitutionYamaguchi University

Principal Investigator

Fujishima Masahiro  山口大学, 大学院創成科学研究科, 教授(特命) (40127783)

Co-Investigator(Renkei-kenkyūsha) KODAMA Yuuki  島根大学, 生物資源科学部, 准教授 (80582478)
Research Collaborator TANAKA Kenya  
MURAKAMI Riko  
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Keywordsミドリゾウリムシ / 共生クロレラ / 二次共生 / 感染 / 細胞内共生 / 食胞 / 真核細胞の進化 / 細胞の進化 / 細胞進化 / モノクローナル抗体 / 進化生物学一般
Outline of Final Research Achievements

Paramecium bursaria is the only experimental system capable of inducing endosymbiosis between eukaryotic cells in synchronization with a large amount of cells. The genes of P. bursaria with markedly altered expression level in the presence or absence of symbiotic Chlorella cells were detected by RNAseq, and the antibodies raised for synthesized petides of the gene products were developed and used to know intracellular localization of the antigens. In addition, an antibody against the perialgal vacuole membrane, which plays an essential role to protect algae from lysosomal fusion in secondary symbiosis, and clarified timing of appearance of the antigen during infection process.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (55 results)

All 2018 2017 2016 2015 2014 Other

All Int'l Joint Research (6 results) Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (34 results) (of which Int'l Joint Research: 1 results,  Invited: 6 results) Book (2 results) Remarks (6 results)

  • [Int'l Joint Research] エクス=マルセイユ大学(フランス)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] シュツッツガルト大学(ドイツ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Aux-Marseille University(フランス)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Pisa University(イタリア)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Innsbruck(オーストリア)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Aix-Marseille University(フランス)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] High degree of specificity in the association between symbiotic betaproteobacteria and the host Euplotes (Ciliophora, Hypotrichia)2017

    • Author(s)
      Claudia Vannini, Cristiana Sigona, Martin Hahn, Giulio Petroni, Masahiro Fujishima
    • Journal Title

      European Journal of Protistology

      Volume: 59 Pages: 124-132

    • DOI

      10.1016/j.ejop.2017.04.003

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Genome Size of the Ultrasmall Unicellular Freshwater Green Alga, <i>Medakamo hakoo</i> 311, as Determined by Staining with 4′,6-Diamidino-2-phenylindole after Microwave Oven Treatments: II. Comparison with <i>Cyanidioschyzon merolae</i>, <i>Saccharomyces cerevisiae</i> (<i>n</i>, 2<i>n</i>), and <i>Chlorella variabilis</i>2016

    • Author(s)
      Kuroiwa, T., Ohnuma, M., Imoto, Y., Misumi, O., Nagata, N., Miyakawa, I., Fujishima, M., Yagisawa, F., Kuroiwa, H.
    • Journal Title

      CYTOLOGIA

      Volume: 81 Issue: 1 Pages: 69-76

    • DOI

      10.1508/cytologia.81.69

    • NAID

      130005143755

    • ISSN
      0011-4545, 1348-7019
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ciliate Paramecium is a natural reservoir of Legionella pneumophila2016

    • Author(s)
      Watanabe K, Nakao R, Fujishima M, Tachibana M, Shimizu T, Watarai M
    • Journal Title

      Sci Rep

      Volume: 6 Issue: 1 Pages: 24322-24322

    • DOI

      10.1038/srep24322

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of amino acid and codon usage in Paramecium bursaria.2015

    • Author(s)
      Hideo Dohra, Masahiro Fujishima, Haruo Suzuki
    • Journal Title

      FEBS Letters

      Volume: 589 Issue: 20PartB Pages: 3113-3118

    • DOI

      10.1016/j.febslet.2015.08.033

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differences in infectivity of endosymbiotic Chlorella variabilis that are cultivated outside of the host Paramecium bursaria for 50 years and immediately after isolation from the host cells.2015

    • Author(s)
      Kodama Y. Fujishima M.
    • Journal Title

      Biology Open

      Volume: - Pages: 1-7

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Symbiotic Chlorella variabilis incubated under constant dark condition for 24 hours loses ability to avoid digestion by host lysosomal enzymes in digestive vacuoles of host ciliate Paramecium bursaria2014

    • Author(s)
      Kodama Y., Fujishima M.
    • Journal Title

      FEMS Microbiology Ecology

      Volume: 90 Issue: 3 Pages: 946-955

    • DOI

      10.1111/1574-6941.12448

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Draft genome sequences of three Holospora species (Holospora obtusa, Holospora undulata, and Holospora elegans), endonuclear symbiotic bacteria of the ciliate Paramecium caudatum2014

    • Author(s)
      Dohra, Hideo; Tanaka, Kenya; Suzuki, Tomohiro; Fujishima, Masahiro; Suzuki, Haruo
    • Journal Title

      FEMS Microbiology Letters (Genome announcements)

      Volume: 359 Issue: 1 Pages: 16-18

    • DOI

      10.1111/1574-6968.12577

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] タカサゴシロアリの腸内共生スピロヘータによるキシラン分解2018

    • Author(s)
      徳田岳、福井知穂、松浦優、渡辺裕文、藤島政博
    • Organizer
      第62回日本応用動物昆虫学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ゾウリムシを用いて細胞内共生のしくみを解明する2018

    • Author(s)
      藤島政博
    • Organizer
      青少年サイエンスセミナー2018春
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 比較ゲノム解析を用いたレジオネラのゾウリムシ共生因子の探索、ワークショップ3「選抜ワークショップ」2018

    • Author(s)
      渡邉健太、西田隆司、橘 理人、藤島政博、清水 隆、度会雅久.
    • Organizer
      第91回日本細菌学会総会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Legionella pneumonia TolC inhibitoscellular trafficking in Paramecium tetraurelia2018

    • Author(s)
      Nishida T, Hara N, Watanabe K, Shimizu T, Fujishima M, Watarai M.
    • Organizer
      第91回日本細菌学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 真核細胞のモデル材料として使用されてきたゾウリムシコレクションの展望2018

    • Author(s)
      藤島政博
    • Organizer
      第91回日本細菌学会総会、ワークショップ7「魅力ある研究素材としての第四期NBRPコレクションの紹介とABS情報の最前線」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] PacBioシーケンスによるゾウリムシ核内共生Ca. Megaira polyxenophilaのゲノム解析2017

    • Author(s)
      道羅英夫、鈴木治夫、鈴木智大、藤島政博
    • Organizer
      NGS現場の会第五回研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] レジオネラのゾウリムシ共生必須因子の同定とその機能解析2017

    • Author(s)
      渡邉健太、三島真渚美、西田隆司、藤島政博、清水隆、度会雅久
    • Organizer
      第160回日本獣医学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Legionella pneumophilaと原生生物との共生におけるTolCの機能解析2017

    • Author(s)
      原奈穂、西田隆司、渡邉健太、藤島政博、清水隆、度会雅久
    • Organizer
      第160回日本獣医学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 共生藻の有無で発現が変化するミドリゾウリムシの遺伝子産物の細胞内局在性2017

    • Author(s)
      藤島政博、児玉有紀
    • Organizer
      第88回日本動物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 「ゾウリムシ」ってどんなムシ?2017

    • Author(s)
      藤島政博、田中健也、シャルコフ真由美、村上理子、中村美紀、児玉有紀
    • Organizer
      第88回日本動物学会、動物学ひろば
    • Related Report
      2017 Annual Research Report
  • [Presentation] ゾウリムシの凍結保存の現状2017

    • Author(s)
      藤島政博
    • Organizer
      Cryopreservation Conference 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 原生生物ゾウリムシ2017

    • Author(s)
      藤島政博、田中健也、村上理子
    • Organizer
      第40回日本分子生物学会、特別企画「NBRP実物つきパネル展示」
    • Related Report
      2017 Annual Research Report
  • [Presentation] ゾウリムシ共生細菌のゲノム情報を利用したレジオネラ属のゾウリムシ共生因子の探索2016

    • Author(s)
      三島真渚美、渡邉健太、鈴木治夫、藤島政博、清水 隆、度会雅久
    • Organizer
      第159回日本獣医学会
    • Place of Presentation
      日本大学生物資源科学部(神奈川県藤沢市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] LefA 遺伝子を介したLegionella pneumophilaの原生生物共生メカニズムの解析2016

    • Author(s)
      渡邉健太、藤島政博、度会雅久
    • Organizer
      第159回日本獣医学会
    • Place of Presentation
      日本大学生物資源科学部(神奈川県藤沢市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] Modulation of endosymbiosis by Legionella pneumophila in Ciliate Paramecium2016

    • Author(s)
      渡邉 健太、中尾 亮、藤島 政博、橘 理人、清水 隆、度会 雅久
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター、大阪府、大阪市
    • Year and Date
      2016-03-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 一次共生の成立機構解明のモデル生物としてのゾウリムシ2016

    • Author(s)
      藤島政博
    • Organizer
      第87回日本動物学会沖縄大会シンポジウム『ナショナルバイオリソースプロジェクト「ゾウリムシ」を用いた研究例』
    • Place of Presentation
      オキナワコンベンションセンター(沖縄県宜野湾市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 「ゾウリムシ」ってどんなムシ?2016

    • Author(s)
      藤島政博、児玉有紀、中村美紀、田中健也
    • Organizer
      第87回日本動物学会沖縄大会動物学ひろば
    • Place of Presentation
      沖縄タイムスビル1Fエントランス(沖縄県宜野湾市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 核内共生細菌ホロスポラが菌体内に取り込む宿主核ヒストンの分子種の特定2015

    • Author(s)
      内田 綺乃、村上 崇史、児玉 有紀、藤島 政博
    • Organizer
      日本原生生物学会第48回東京大会
    • Place of Presentation
      国立感染症研究所、東京都、新宿区
    • Year and Date
      2015-11-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞内共生の成立機構の解明と宿主細胞の環境適応力の増強2015

    • Author(s)
      藤島 政博
    • Organizer
      第30回微生物生態学会(土浦大会)
    • Place of Presentation
      土浦市亀城プラザ、土浦市、茨城県
    • Year and Date
      2015-10-20
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] UV induces transfer of 16S rRNA fragments of the micronucleus-specific bacterium Holospora undulata to the host Paramecium nucleoli.2015

    • Author(s)
      Fujishima M.
    • Organizer
      VII European Congress of Protistology
    • Place of Presentation
      Seville University, Seville, Spain
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Plenary lecture. Endosymbiosis in Paramecium bursaria and Chlorella variabilis2014

    • Author(s)
      Fujishima M
    • Organizer
      2nd Asian Congress of Protistology
    • Place of Presentation
      Karyani Univ., India (Karyani市)
    • Year and Date
      2014-11-29
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Ultraviolet ray induces transfer of 16S rRNA fragments of micronucleus-specific bacterium Holospora to the host Paramecium macronuclear nucleoli2014

    • Author(s)
      Yuki Kawamoto, Masahiro Fujishima
    • Organizer
      2nd Asian Congress of Protistology
    • Place of Presentation
      Karyani Univ., India (Karyani市)
    • Year and Date
      2014-11-27 – 2014-11-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] ミドリゾウリムシのトランスクリプトームデータを用いたゲノム機能解析2014

    • Author(s)
      道羅 英夫・児玉 有紀・鈴木 治夫・杉井 学・北爪 達也・山口 勝司・重信 秀治・藤島 政博
    • Organizer
      日本原生生物学会
    • Place of Presentation
      宮城教育大学(仙台市)
    • Year and Date
      2014-10-31 – 2014-11-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] ミドリゾウリムシとクロレラの細胞内共生初期過程におけるPerialgal vacuole膜の分化時期2014

    • Author(s)
      藤島 政博・山下 淳平 ・児玉 有紀
    • Organizer
      日本原生生物学会
    • Place of Presentation
      宮城教育大学(仙台市)
    • Year and Date
      2014-10-31 – 2014-11-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] An investigation on the role of betaproteobacterial endosymbionts in Euplotes for a better understanding of symbiosis evolution: preliminary results of trans-infection experiments2014

    • Author(s)
      CRISTIANA SIGONA, MASAHIRO FUJISHIMA, GIULIO PETRONI, MARTIN HAHN, CLAUDIA VANNINI
    • Organizer
      29th Italian Society of Protistology
    • Place of Presentation
      Italy (Padova市)
    • Year and Date
      2014-10-02 – 2014-10-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] 紫外線はゾウリムシの小核特異的共生細菌ホロスポラの16S rRNA断片を宿主大核核小体に転移させる2014

    • Author(s)
      河本雄貴,佐藤晋也,藤島政博
    • Organizer
      日本動物学会第85回仙台大会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゾウリムシ属における油産生能力の比較2014

    • Author(s)
      田中健也、藤島政博
    • Organizer
      日本動物学会第85回仙台大会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 感染初期過程に置けるクロレラの細胞分裂と細胞数は宿主ミドリゾウリムシの栄養状態で調節される2014

    • Author(s)
      児玉有紀、藤島政博
    • Organizer
      日本動物学会第85回仙台大会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] ミドリゾウリムシとクロレラの細胞内共生初期過程におけるクロレラ包膜の分化時期2014

    • Author(s)
      藤島政博、山下淳平、児玉有紀
    • Organizer
      日本動物学会第85回仙台大会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゾウリムシ感染モデルを用いたレジオネラ属菌の原生生物内寄生機序の解析2014

    • Author(s)
      渡邉健太、橘理人、南川薫、藤島政博、清水隆、度会雅久
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 紫外線UV-Cの照射によって誘導されるゾウリムシの小核特異的共生細菌Holospora undulataの16SリボソームRNA断片の宿主大核核小体への転移2014

    • Author(s)
      河本雄貴,佐藤晋也,藤島政博
    • Organizer
      生物系三学会中四国支部大会
    • Place of Presentation
      岡山理科大学(岡山市)
    • Year and Date
      2014-05-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゾウリムシの小核特異的共生細菌Holospora undulataのペリプラズムに存在する高分子量抗原の性質2014

    • Author(s)
      寺田弥生,藤島政博
    • Organizer
      生物系三学会中四国支部大会
    • Place of Presentation
      岡山理科大学(岡山市)
    • Year and Date
      2014-05-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] ミドリゾウリムシの共生クロレラ包膜特異的モノクローナル抗体の作成2014

    • Author(s)
      名原 諒,藤島政博
    • Organizer
      生物系三学会中四国支部大会
    • Place of Presentation
      岡山理科大学(岡山市)
    • Year and Date
      2014-05-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] ミドリゾウリムシの共生クロレラ包膜と宿主細胞表層直下のミトコンドリアとの接着2014

    • Author(s)
      藤島政博,西山 翔,児玉有紀
    • Organizer
      生物系三学会中四国支部大会
    • Place of Presentation
      岡山理科大学(岡山市)
    • Year and Date
      2014-05-10
    • Related Report
      2014 Annual Research Report
  • [Book] Biocommunication in ciliates. Chapter 16. Paramecium as a model organism for studies on primary and secondary endosymbioses.2016

    • Author(s)
      Kodama Y., Fujishima M.
    • Total Pages
      372
    • Publisher
      Springer International Publishing Switzerland
    • Related Report
      2016 Annual Research Report
  • [Book] Biocommunication of Ciliates.2016

    • Author(s)
      Kodama Y., Fujishima M.
    • Publisher
      Springer
    • Related Report
      2015 Annual Research Report
  • [Remarks] FUJISHIMA Lab HOMEPAGE

    • Related Report
      2017 Annual Research Report
  • [Remarks] FUJISHIMA LAB

    • Related Report
      2016 Annual Research Report
  • [Remarks] ナショナルバイオリソースプロジェクト・ゾウリムシ

    • URL

      http://nbrpcms.nig.ac.jp/paramecium/

    • Related Report
      2016 Annual Research Report
  • [Remarks] FUJISHIMA Lab Homepage

    • Related Report
      2015 Annual Research Report
  • [Remarks] FUJISHIMA LAB HOMEPAGE

    • Related Report
      2014 Annual Research Report
  • [Remarks] 山口大学研究推進体 微生物の機能進化と環境適応

    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2024-03-04  

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