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Studies on genomic structure and evolution of spinach sex-determining loci

Research Project

Project/Area Number 26292001
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Science in genetics and breeding
Research InstitutionHokkaido University

Principal Investigator

Onodera Yasuyuki  北海道大学, (連合)農学研究科(研究院), 講師 (80374619)

Co-Investigator(Kenkyū-buntansha) 近江戸 伸子  神戸大学, 人間発達環境学研究科, 教授 (30343263)
星野 洋一郎  北海道大学, 北方生物圏フィールド科学センター, 准教授 (50301875)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Keywords植物育種 / F1ハイブリッド / ホウレンソウ / 性決定遺伝子 / 間性 / 雌雄異株 / 育種 / 性染色体
Outline of Final Research Achievements

Spinach is commonly known as a dioecious species. However, certain lines and crosses produce monoecious individuals. Dioecism and monoecism are of relevance in commercial hybrid seed production, and elucidation of the mechanisms underlying sex determination is valuable for spinach breeding programs. Sex expression in dioecious spinach plants is controlled by a single gene pair termed X and Y. Meiotic recombination was found suppressed in the vicinity of the male-determining locus (Y). A locus controlling the monoecious condition (M) is linked to X/Y locus at 12 cM.
In this study, we identified 220 genes linked to X/Y locus, part of which were found to be located in the recombinationally suppressed region. The locus for monoecism was confined to a 25-kbp chromosomal segment.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (10 results)

All 2017 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (8 results)

  • [Journal Article] Comparison of spinach sex chromosomes with sugar beet autosomes reveals extensive synteny and low recombination at the male-determining locus2016

    • Author(s)
      Takahata S., Yago T., Iwabuchi K., Hirakawa H., Suzuki Y., Onodera Y
    • Journal Title

      Journal of Heredity

      Volume: 107 Issue: 7 Pages: 679-685

    • DOI

      10.1093/jhered/esw055

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Evidence for a Common Origin of Homomorphic and Heteromorphic Sex Chromosomes in Distinct Spinacia Species.2015

    • Author(s)
      Fujito S, Takahata S, Suzuki R, Hoshino Y, Ohmido N, Onodera Y.
    • Journal Title

      G3

      Volume: 5 Issue: 8 Pages: 1663-1673

    • DOI

      10.1534/g3.115.018671

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] Spinacia属から見出された雑種不稔性および限性遺伝を示す花序形質に関する遺伝学的特徴づけ2017

    • Author(s)
      高橋光彦,小野寺 康之
    • Organizer
      日本育種学会 第131回講演会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2017-03-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] ホウレンソウの間性主働遺伝子座乗候補領域のゲノム構造解析2016

    • Author(s)
      岩渕 恵佑, 杉村 逸郎, 小野寺 康之
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] ホウレンソウ性染色体とテンサイ常染色体の比較解析2016

    • Author(s)
      高畠 聡史,岩渕 恵佑,平川英樹,小野寺 康之
    • Organizer
      日本育種学会 第129回講演会
    • Place of Presentation
      横浜市立大学(横浜市)
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] 岩渕 恵佑,高畠 聡史,小野寺 康之:ホウレンソウの間性主働遺伝子座の精密マッピングと物理地図作成2015

    • Author(s)
      岩渕 恵佑,高畠 聡史,小野寺 康之
    • Organizer
      日本育種学会 第128回講演会
    • Place of Presentation
      新潟大学(新潟県 新潟市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] トランスクリプトーム情報を利用したホウレンソウ性連鎖遺伝子群の網羅的同定2015

    • Author(s)
      高畠 聡史, 平川 英樹, 鈴木 穣, 小野寺 康之
    • Organizer
      日本育種学会 第127回講演会
    • Place of Presentation
      玉川大学(東京都町田市)
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] ホウレンソウY染色体特異的領域に由来するBACクローンの全長配列解析2015

    • Author(s)
      工藤 友裕,藤戸 聡史,豊田 敦,鈴木 穣,小野寺 康之
    • Organizer
      日本育種学会 第127回講演会
    • Place of Presentation
      玉川大学(東京都町田市)
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 系統的に異なるSpinacia属植物からの同型および異型性染色体の同定2014

    • Author(s)
      藤戸 聡史, 鈴木 伶実, 星野 洋一郎, 近江戸 伸子, 小野寺 康之
    • Organizer
      第37回 日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] テンサイとのシンテニーを利用したホウレンソウ性染色体の構造解析2014

    • Author(s)
      高畠 聡史, 矢後 拓己, 本間 雄二朗, 久保 友彦, 小野寺 康之
    • Organizer
      日本育種学会 第126回講演会
    • Place of Presentation
      南九州大学(宮崎市)
    • Year and Date
      2014-09-26 – 2014-09-27
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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