Generation and customization of plant artificial chromosomes in cereals.
| Project/Area Number |
26292005
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Okayama University |
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Principal Investigator |
Murata Minoru 岡山大学, 資源植物科学研究所, 教授 (20166292)
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| Co-Investigator(Kenkyū-buntansha) |
長岐 清孝 岡山大学, その他部局等, 准教授 (70305481)
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| Co-Investigator(Renkei-kenkyūsha) |
KASHIHARA Kazunari 岡山大学, 資源植物科学研究所, 技術専門職員 (60379807)
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| Research Collaborator |
SATO Kazuhiro 岡山大学, 資源植物科学研究所, 教授
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Keywords | 植物人工染色体 / 染色体変異 / ゲノム再構成 / 配列特異的組換え / Cre/LoxP / トランスポゾン / イネ / オオムギ / 植物人工環状染色体 / 転移酵素 / TAIL-PCR / 育種学 / 植物 / バイオテクノロジー / ベクター / 組換え植物 |
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| Outline of Final Research Achievements |
The objective of this study is to develop efficient methods for generation of plant artificial chromosomes that are able to accept a number of genes. The newly designed T-DNA construct (pDLHC) contained two LoxPs, one of which is transposable by a transposase. This transposition was designed to induce expression of the Cre recombinase gene, resulting in site-specific recombination between two separated LoxPs. Among the progeny of rice plants transformed with pDLHC, one transformant was found to contain a large deletion on chromosome 10, which was caused by elimination of a DNA molecule circled by recombination between two LoxPs. If the centromere functions would be given to the circular DNA, artificial ring chromosomes would be generated.
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Report
(4 results)
Research Products
(14 results)
