Molecular basis of postharvest stay-green and its application to breeding

• Project/Area Number: 26292006
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Hiroshima University
• Principal Investigator: Kusaba Makoto 広島大学, 理学研究科, 教授 (20370653)
• Research Collaborator: SAKAMOTO Koji
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000); Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
• Keywords: 老化 / クロロフィル分解 / 光受容体 / 遺伝学 / 植物 / 生理学 / 植物ホルモン / 育種学

Outline of Final Research Achievements

In leaf vegetables including broccoli, postharvest yellowing is a major issue in terms of distribution cost. It will be useful to isolate and analyze stay-green genes, which prevent yellowing under senescence-inducing conditions, for resolution of the issue by genetic improvement. Here we isolated three stay-green genes, cytG in soybean, pif5 in Arabidopsis, and dye1 in rice, and analyzed their molecular functions.

Research Products

• [Journal Article] Impairment of Lhca4, a subunit of LHCI, causes high accumulation of chlorophyll and the stay-green phenotype in rice (2018)
  • Author(s): Hiroshi Yamatani, Kaori Kohzuma, Michiharu Nakano, Tsuneaki Takami, Yusuke Kato, Yoriko Hayashi, Yuki Monden, Yutaka Okumoto, Tomoko Abe Toshihiro Kumamaru, Ayumi Tanaka, Wataru Sakamoto, Makoto Kusaba
  • Journal Title: Journal of Experimental Botany Volume: 5 Pages: 1027-1035
  • NAID: 120006466618
  • Peer Reviewed
• [Journal Article] The non-Mendelian green cotyledon gene in soybean encodes a small subunit of Photosystem II. (2017)
  • Author(s): Kohzuma, K., Sato, Y., Ito, H., Okuzaki, A., Masuda, Y., Watanabe, M., Fukuoka, H., Yamatani, H., Kanazawa, A., Tabei, Y., Ikeuchi, M., Sakamoto, W., Tanaka, A., and Kusaba, M.
  • Journal Title: Plant Physiology Volume: 173 Issue: 4 Pages: 2138-2147
  • DOI: 10.1104/pp.16.01589
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Strigolactone regulates leaf senescence in concert with ethylene in Arabidopsis. (2015)
  • Author(s): Ueda, H. and Kusaba, M
  • Journal Title: Plant Physiology Volume: 55 Issue: 1 Pages: 1763-1771
  • DOI: 10.1104/pp.15.00325
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] イネLHCIサブユニット 機能低下により導かれるクロロフィル高蓄積機構の解析 (2018)
  • Author(s): 山谷 浩史, 上妻 馨梨, 中野 道治, 高見 常明, 田中歩, 坂本 亘, 草場 信
  • Organizer: 第133回日本育種学会
• [Presentation] イネstay-green遺伝子DCD1の単離と機能解析 (2017)
  • Author(s): 山谷浩史, 上妻馨梨, 中野道治, 林依子, 高見常明, 門田有希，奥本裕，坂本亘，阿部知子, 草場信
  • Organizer: 日本育種学会第131回講演会
  • Place of Presentation: 名古屋大学, 名古屋
  • Year and Date: 2017-03-29
• [Presentation] Molecular genetic analysis of the rice stay-green mutant dcd1 (2017)
  • Author(s): Hiroshi Yamatani, Kaori Kohzuma, Michiharu Nakano, Yoriko Hayashi, Tsuneaki Takami, Yusuke Kato, Wataru Sakamoto, Tomoko Abe, Makoto Kusaba
  • Organizer: Plant Biology 2017
  • Int'l Joint Research
• [Presentation] 葉老化時におけるLHCII分解への光化学系II小サブユニットの関与 (2016)
  • Author(s): 上妻馨梨，伊藤寿，渡辺麻衣，池内昌彦，坂本亘，田中歩，草場信
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡）
  • Year and Date: 2016-03-18
• [Presentation] 光環境応答による葉老化制御機構の生理学的解析 (2016)
  • Author(s): 小塚俊明，下野起将，井上良平，草場信
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡）
  • Year and Date: 2016-03-18
• [Presentation] A cytoplasmic stay-green gene in soybean (2016)
  • Author(s): Makoto Kusaba
  • Organizer: 8th International Symposium on Plant Senescence
  • Place of Presentation: Jeju, Korea
  • Int'l Joint Research / Invited
• [Presentation] Nuclear and cytoplasmic stay-green genes in legume. (2015)
  • Author(s): Makoto Kusaba
  • Organizer: International Symposium on Dynamics and Regulation of Photosynthesis
  • Place of Presentation: 奈良春日野国際フォーラム（奈良）
  • Year and Date: 2015-10-29
  • Int'l Joint Research / Invited
• [Presentation] エチレン合成とストリゴラクトン合成の二段階制御を介した葉老化促進 (2015)
  • Author(s): 上田浩晶・草場信
  • Organizer: 日本育種学会
  • Place of Presentation: 東京（玉川大学）
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] Phytochrome Interacting Factor 4・5による葉老化制御機構の解析 (2015)
  • Author(s): 井上良平・宮田麗華・山谷浩史・高木優・草場信
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京（東京農業大学）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] ストリゴラクトンを介した葉老化制御の解析 (2015)
  • Author(s): 上田浩晶・草場信
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京（東京農業大学）
  • Year and Date: 2015-03-16 – 2015-03-18