Study on the regulatory mechanisms of floral organ size determination in rice.

• Project/Area Number: 26292008
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Yoshida Hitoshi 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門 遺伝子利用基盤研究領域, ユニット長 (30355565)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000); Fiscal Year 2016: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: イネ / 発生・分化 / 花器官 / サイズ制御 / 変異体 / メリステム / MIF遺伝子 / SAN遺伝子 / 育種学 / 遺伝学 / 遺伝子

Outline of Final Research Achievements

In order to elucidate the molecular mechanism underlying the regulation of floral organ (FO) size in rice, we analyzed two mutants; 1) miniature floral organs (mif) in which the NUMBER of FOs are not altered, but the SIZE of FOs are reduced to less than half of wild type, 2) short anther (san) in which the stamens (especially the anthers) are specifically shortened. Double mutants between mif and fon1 in which the floral meristem (FM) are enlarged showed small FOs, indicating regulatory mechanisms between FM size and FO size are independent. We also identified that the MIF gene encodes a member of chromatin remodeler-like proteins, and SAN encodes an adapter-like protein.

Research Products

• [Journal Article] The superwoman1-cleistogamy2 mutant is a novel resource for gene containment in rice (2017)
  • Author(s): Fabien Lombardo, Makoto Kuroki, Shan-Guo Yao, Hiroyuki Shimizu, Tomohito Ikegaya, Mayumi Kimizu, Shinnosuke Ohmori, Takashi Akiyama, Takami Hayashi, Tomoya Yamaguchi, Setsuo Koike, Osamu Yatou and Hitoshi Yoshida
  • Journal Title: Plant Biotechnology Journal Volume: 15 Issue: 1 Pages: 97-106
  • DOI: 10.1111/pbi.12594
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cleistogamy Decreases the Effect of High Temperature Stress at Flowering in Rice (2015)
  • Author(s): Setsuo Koike, Tomoya Yamaguchi, Shinnosuke Ohmori, Takami Hayashi, Osamu Yatou, Hitoshi Yoshida
  • Journal Title: Plant Production Science Volume: 18
  • NAID: 130005129891
  • Peer Reviewed / Open Access
• [Journal Article] Interpreting lemma and palea homologies: a point of view from rice floral mutants (2015)
  • Author(s): Fabien Lombardo and Hitoshi Yoshida
  • Journal Title: Frontiers in Plant Science Volume: 6
  • DOI: 10.3389/fpls.2015.00061
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] イネの形態形成と耐病性に関わるDDR1遺伝子 (2017)
  • Author(s): 吉田均、松村葉子、森浩一、山元剛、中島敏彦、Fabien Lombardo、秋山高、川田元滋
  • Organizer: 日本育種学会
  • Place of Presentation: 名古屋大学（愛知県・名古屋市）
  • Year and Date: 2017-03-29
• [Presentation] Inner floral organ development is perturbed in the rice miniature floral organs mutant. (2017)
  • Author(s): 吉田均、Fabien Lombardo、秋山高、佐藤豊
  • Organizer: 日本植物生理学会
  • Place of Presentation: 鹿児島大学（鹿児島県・鹿児島市）
  • Year and Date: 2017-03-15
• [Presentation] 温度勾配チャンバーを用いたイネ閉花受粉性突然変異体spw1-clsの温度反応性評価 (2016)
  • Author(s): 林高見,山口知哉,小池説夫,黒木慎,池ヶ谷智仁,清水博之,大森伸之介,福嶌陽,吉田均
  • Organizer: 日本育種学会
  • Place of Presentation: 横浜市立大学
  • Year and Date: 2016-03-21
• [Presentation] イネ閉花受粉性突然変異体spw1-cls2の圃場条件における特性評価 (2016)
  • Author(s): 黒木慎,池ヶ谷智仁,清水博之,林高見,福嶌陽,山口知哉,大森伸之介,吉田均
  • Organizer: 日本育種学会
  • Place of Presentation: 横浜市立大学
  • Year and Date: 2016-03-21
• [Presentation] ガンマ線で誘発したイネ閉花受粉性変異体H193mtにおける複雑なゲノム再編成 (2016)
  • Author(s): 大森伸之介、吉田均
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] 2つの新規閉花受粉性イネ突然変異体の原因遺伝子のマッピング (2015)
  • Author(s): 大森伸之介、吉田均
  • Organizer: 日本育種学会
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] Fine-tuning the floral phenotypes in rice: narrow but deep gaps between mutations and agronomic traits (2014)
  • Author(s): Hitoshi Yoshida
  • Organizer: KAAB International Symposium 2014 “Frontiers in Plant Science and Biotechnology”
  • Place of Presentation: 新潟大学（新潟県新潟市）
  • Year and Date: 2014-09-29
  • Invited
• [Presentation] イネにおけるＢクラス変異のもたらす閉花性と稔実率とのtrade-off (2014)
  • Author(s): 吉田均,黒木慎,姚善国,Lombardo Fabien,清水博之,池ヶ谷智仁,木水真由美,大森伸之介,秋山高,林高見,小池説夫,矢頭治
  • Organizer: 日本育種学会
  • Place of Presentation: 南九州大学（宮崎県都城市）
  • Year and Date: 2014-09-26 – 2014-09-27
• [Presentation] Ｂクラス変異のもたらす閉花性と稔実率とのtrade-off (2014)
  • Author(s): 吉田均,黒木慎,姚善国,Lombardo Fabien,清水博之,池ヶ谷智仁,木水真由美,大森伸之介,秋山高,林高見,小池説夫,矢頭治
  • Organizer: イネ遺伝学・分子生物学ワークショップ 2014
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2014-07-11 – 2014-07-12
• [Patent(Industrial Property Rights)] 短葯形質を有するイネ、及びその製造方法 (2022)
  • Inventor(s): 吉田均, 黒羽剛, ロンバルド フェビエン
  • Industrial Property Rights Holder: 国立研究開発法人農業・食品産業技術研究開発機構
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2022-013003
  • Filing Date: 2022