Physiological roles and molecular mechanisms of abscisic acid-responsive chloroplast aggregative movement specific to C4 plants

• Project/Area Number: 26292011
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Crop production science
• Research Institution: Nagoya University
• Principal Investigator: TANIGUCHI Mitsutaka 名古屋大学, 生命農学研究科, 教授 (40231419)
• Co-Investigator(Renkei-kenkyūsha): SAKAKIBARA Hitoshi 独立行政法人理化学研究所, 環境資源科学研究センター, グループディレクター (20242852)
• Research Collaborator: OI Takao 名古屋大学, 大学院生命農学研究科, 助教 (60752219)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2016: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: C4植物 / 葉緑体 / 凝集運動 / アブシジン酸 / 青色光 / エノコログサ / シコクビエ / 環境ストレス / 植物 / ストレス / 光合成 / ホルモン / スクリーニング

Outline of Final Research Achievements

Progeny seeds of green foxtail mutagenized by heavy ion beam irradiation were collected. We have established a screening method for mutants that do not cause abscisic acid-responsive aggregative movement of mesophyll chloroplasts with the mutant library. We also revealed that the aggregative arrangement in mesophyll cells occurs even in plastids under the dark conditions or in the early stage of development, while a mechanism for dispersing the chloroplasts is working under the non-stressed light conditions.

Research Products

• [Journal Article] Editorial: Future research into C4 biology (2016)
  • Author(s): Taniguchi M,, Weber A.P.M., von Caemmerer S.
  • Journal Title: Plant & Cell Physiology Volume: 57 Issue: 5 Pages: 879-880
  • DOI: 10.1093/pcp/pcw082
  • Int'l Joint Research
• [Journal Article] Interactions of C4 subtype metabolic activities and transport in maize are revealed through the characterization of DCT2 mutants. (2016)
  • Author(s): Weissmann S, Ma F, Furuyama K, Gierse J, Berg H, Shao Y, Taniguchi M, Allen DK, Brutnell TP.
  • Journal Title: Plant Cell Volume: 28 Issue: 2 Pages: 466-484
  • DOI: 10.1105/tpc.15.00497
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Salinity induces membrane structure and lipid changes in maize mesophyll and bundle sheath chloroplasts. (2016)
  • Author(s): Omoto E, Iwasaki Y, Miyake H, Taniguchi M.
  • Journal Title: Physiologia Plantarum Volume: 157 Issue: 1 Pages: 13-23
  • DOI: 10.1111/ppl.12404
  • Peer Reviewed
• [Journal Article] C4植物におけるストレス応答の細胞特異性 (2016)
  • Author(s): 谷口光隆，三宅博
  • Journal Title: 植物科学最前線 (BSJ-Review) Volume: 7 Pages: 2-11
  • Open Access
• [Presentation] C4植物葉肉葉緑体の凝集運動に伴う配置変化の三次元観察の試み (2017)
  • Author(s): 山川早紀、大井崇生、谷口光隆
  • Organizer: 日本光合成学会
  • Place of Presentation: 大津
  • Year and Date: 2017-05-27
• [Presentation] 長期暗条件により誘導されるC4植物葉肉葉緑体の凝集配置 (2016)
  • Author(s): 塚口駿貴，加藤優太，大井崇生，谷口光隆
  • Organizer: 日本作物学会第241回講演会
  • Place of Presentation: 水戸
  • Year and Date: 2016-03-28
• [Presentation] C4植物における葉肉葉緑体の細胞内配置: 葉組織成長に伴う凝集配置から光受容に伴う分散配置への変化 (2016)
  • Author(s): 加藤優太，塚口駿貴，大井崇生，谷口光隆
  • Organizer: 日本作物学会第241回講演会
  • Place of Presentation: 水戸
  • Year and Date: 2016-03-28
• [Presentation] C4植物葉肉葉緑体凝集運動の効率的観察法 (2015)
  • Author(s): 堤浩一，宗像里美，大井崇生，木羽隆敏，榊原均，谷口光隆
  • Organizer: 日本作物学会第239回講演会
  • Place of Presentation: 藤沢
  • Year and Date: 2015-03-27 – 2015-03-28