| Budget Amount *help |
¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Outline of Final Research Achievements |
The diversity of carotenoid cleavage dioxygenase genes (CCD4s)
which operates specifically in ray florets of Chrysanthemum was analyzed. Although the CCD4 genes contain minor nucleotide sequence mutations, having high sequence similarlities of more than 90% in both Chrysanthemum cultivars and wild species, overall, they showed diversity. In Chrysanthemum cultivars at least 8 kinds of CCD4 homologs existed in their genome when classified by sequence similarity of 98% or more, 6 of which were expressed in ray florets. In the diploid Chrysanthemum wild species, Chrysanthemum makinoi, CCD4 homologs similar to CmCCD4a-5 of the cultivar‘Jimba’ were expressed. CCD4 gene fragments, which have been thought not to exist in the genomes of yellow flowered cultivars and wild species, have also been found.
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