Molecular basis of immune receptor conferring dual resistance to virus and oomycete and its application for disease control

• Project/Area Number: 26292022
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Tohoku University
• Principal Investigator: Takahashi Hideki 東北大学, 農学研究科, 教授 (20197164)
• Co-Investigator(Kenkyū-buntansha): 安藤 杉尋 東北大学, 農学研究科, 准教授 (10442831)
• Project Period (FY): 2014-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
• Keywords: ウイルス / NB-LRRクラス抵抗性タンパク質 / エピジェネティクス / 病害抵抗性 / NB-LRRタンパク質 / ウィルス / 植物保護 / NB-LRR class Rタンパク質 / 植物病理 / ウイルス抵抗性 / キュウリモザイクウイルス / 病害抵抗性遺伝子

Outline of Final Research Achievements

RCY1/HRT/RPP8 locus of Arabidopsis thaliana encodes NB-LRR class R-protein conferring the resistance to CMV, TCV and Hpa. The expression of RCY1/HRT/RPP8 locus was regulated by epigenetic modification through cytosine methylation of its promoter region, and accumulated level of R-protein was correlated with degree of disease resistance to pathogens. RCY1/HRT/RPP8 R-protein can recognize the molecular structure of multiple complex of CP mediated by N-terminal α-helix domain of CP.

Academic Significance and Societal Importance of the Research Achievements

(1)抵抗性対立遺伝子座RCY1、HRT、RPP8は、エピジェネティックな調節により発現制御を受けていること、(2)同遺伝子座がコードするNB-LRRクラス抵抗性タンパク質の病原体認識にはLRRドメインが関わっていること、(3)同抵抗性タンパク質による認識される病原体分子（非病原力因子）の高次構造を明らかにした。これらの知見は抵抗性対立遺伝子座RCY1、HRT、RPP8による病害抵抗性の分子基盤の理解と、新規な病害防除方法への応用に寄与するものと考えられる。

Research Products

• [Int'l Joint Research] ワーゲニンゲン大学(オランダ)
• [Int'l Joint Research] カフルエルシェイク大学(エジプト)
• [Journal Article] First identification and characterization of cucumber mosaic virus from Corchorus olitorius in Japan (2018)
  • Author(s): Hanbal Sara E.、Miyashita Shuhei、Ando Sugihiro、Sidaros Samir A.、Takahashi Hideki
  • Journal Title: Journal of Plant Pathology Volume: 100 Issue: 3 Pages: 561-565
  • DOI: 10.1007/s42161-018-0099-6
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Survey of the response of 82 domestic landraces of Zea mays to cucumber mosaic virus reveals geographical region-related resistance to CMV in Japan (2018)
  • Author(s): Takahashi H.、Tian A.、Miyashita S.、Kanayama Y.、Ando S.、Kormelink R.
  • Journal Title: Plant Pathology Volume: in press Issue: 6 Pages: 1401-1415
  • DOI: 10.1111/ppa.12848
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Increased cytosine methylation at promoter of the NB-LRR class R gene RCY1 correlated with compromised resistance to cucumber mosaic virus in EMS-generated src mutants of Arabidopsis thaliana (2017)
  • Author(s): Sato Yukiyo、Miyashita Shuhei、Ando Sugihiro、Takahashi Hideki
  • Journal Title: Physiol Mol Plant Pathol Volume: 100 Pages: 151-162
  • DOI: 10.1016/j.pmpp.2017.09.007
  • Peer Reviewed / Open Access
• [Journal Article] L-Histidine induces resistance in plants to the bacterial pathogen Ralstonia solanacearum partially through the activation of ethylene signaling. (2016)
  • Author(s): Seo, S., Nakaho, K., Hong, S-W., Takahashi, H., Shigemori, H. and Mitsuhara, I.
  • Journal Title: Plant Cell Physiology Volume: 57 Issue: 9 Pages: 1932-1942
  • DOI: 10.1093/pcp/pcw114
  • NAID: 120006771684
  • Peer Reviewed
• [Journal Article] Involvement of NtERF3 in the cell death signalling pathway mediated by SIPK/WIPK and WRKY1 in tobacco plants. (2015)
  • Author(s): Ogata, T., Okada, H., Kawaide, H., Takahashi, H., Seo, S., Mitsuhara, I. and Matsushita, Y.
  • Journal Title: Plant Biology Volume: 17 Issue: 5 Pages: 962-972
  • DOI: 10.1111/plb.12349
  • Peer Reviewed
• [Journal Article] Cauliflower mosaic virus Tav protein induces leaf chlorosis in transgenic tobacco through a host response to its virulence function. (2015)
  • Author(s): Waliullah S, Kosaka N, Yaeno T, Ali ME, Sekine KT, Atsumi G, Yamaoka N, Nishiguchi M, Takahashi H and Kobayashi K
  • Journal Title: Journal of General Plant Pathology Volume: - Issue: 4 Pages: 261-270
  • DOI: 10.1007/s10327-015-0600-4
  • Peer Reviewed
• [Journal Article] Role of intron-mediated enhancement on accumulation of an Arabidopsis NB-LRR class R-protein that confers resistance to Cucumber mosaic virus. (2014)
  • Author(s): Sato, Y., Ando, S. and Takahashi, H.
  • Journal Title: PLoS ONE Volume: 9 Issue: 9 Pages: e107185-e107185
  • DOI: 10.1371/journal.pone.0107185
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transcriptional profile of tomato roots exhibiting Bacillus thuringiensis- induced resistance to Ralstonia solanacearum. (2014)
  • Author(s): Takahashi, H., Nakaho, K., Ishihara, T., Ando, S., Wada, T., Kanayama, Y., Asano, S., Yoshida, S., Tsushima, S. and Hyakumachi, M.
  • Journal Title: Plant Cell Reports Volume: 33 Pages: 99-110
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] WRKY70 interacting with RCY1 disease resistance protein is required for resistance to Cucumber mosaic virus in Arabidopsis thaliana. (2014)
  • Author(s): Ando, S., Obinata, A. and Takahashi, H.
  • Journal Title: Physiological and Molecular Plant Pathology Volume: 85 Pages: 8-14
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] エジプト産ササゲ品種EGのキュウリモザイクウイルス高度抵抗性を決定するウイルス因子の解析 (2019)
  • Author(s): 村山友理・井尾美由記・安藤杉尋・宮下脩平・Sara E. Hanbal・高橋英樹
  • Organizer: 平成31年度日本植物病理学会大会
• [Presentation] キュウリモザイクウイルス(CMV)に対するエジプト産ササゲ品種EGの応答とCMVに対する高度抵抗性の性状解析 (2019)
  • Author(s): 井尾美由記・村山友理・安藤杉尋・宮下脩平・Sara E. Hanbal・高橋英樹
  • Organizer: 平成31年度日本植物病理学会大会
• [Presentation] Analysis of genomic region in cucumber mosaic virus RNA1 which determines the induction of the non-HR cell death in reassorted CMV-inoculated leaves of Arabidopsis thaliana (2019)
  • Author(s): Ainan Tian・Shuhei Miyashita・Sugihiro Ando・Hideki Takahashi
  • Organizer: 平成31年度日本植物病理学会大会
• [Presentation] BTH処理によるRNAサイレンシング関連因子AGO2のプライミングを制御する転写因子の探索 (2019)
  • Author(s): 安藤杉尋・小関彩恵子・宮下脩平・高橋英樹
  • Organizer: 平成31年度日本植物病理学会大会
• [Presentation] キュウリモザイクウイルスに不顕性感染したシロイヌナズナにおける2bタンパク質の役割 (2019)
  • Author(s): 高橋英樹・田原 緑・宮下脩平・安藤杉尋・福原敏行
  • Organizer: 平成31年度日本植物病理学会大会
• [Presentation] Latent plant-virus interactions and their impact on plant stress tolerance. (2018)
  • Author(s): Hideki Takahashi
  • Organizer: WUR-TU Plant Science Workshop 2018
  • Int'l Joint Research / Invited
• [Presentation] Comparative analysis of two-type of cell death developed in cucumber mosaic virus-inoculated leaves of Arabidopsis thaliana (2018)
  • Author(s): Ainan Tian, Shuhei Miyashita, Sugihiro Ando, Hieki Takahashi
  • Organizer: 平成30年度日本植物病理学会大会
• [Presentation] R遺伝子による認識を免れる変異型CMV CPのアラニンスキャニングによる探索 (2018)
  • Author(s): 西條悠希, 安藤杉尋, 高橋英樹, 宮下脩平
  • Organizer: 平成30年度日本植物病理学会大会
• [Presentation] シロイヌナズナへの無病徴感染に関わるキュウリモザイクウイルスのゲノムRNAと宿主因子の解析 (2018)
  • Author(s): 吉澤 峻, 宮下脩平, 安藤杉尋, 福原敏行, 高橋英樹
  • Organizer: 平成30年度日本植物病理学会大会
• [Presentation] キュウリモザイクウイルスに無病徴感染したシロイヌナズナにおけるRNA-seq解析 (2018)
  • Author(s): 高橋英樹, 田原 緑, 平山裕也, 宮下脩平, 安藤杉尋, 川野修一, 福原敏行
  • Organizer: 平成30年度日本植物病理学会大会
• [Presentation] EMS変異誘発シロイヌナズナ系統におけるNB-LRRクラス抵抗性遺伝子RCY1プロモーター領域シトシンメチル化とCMV抵抗性レベル低下の相関 (2017)
  • Author(s): 佐藤有希代・宮下脩平・安藤杉尋・高橋英樹
  • Organizer: 平成29年度日本植物病理学会大会
• [Presentation] ヒストン脱メチル化酵素変異体ldlにおけるキュウリモザイクウイルス抵抗性の解析 (2017)
  • Author(s): 小関彩恵子・宮下脩平・高橋英樹・安藤杉尋
  • Organizer: 平成29年度日本植物病理学会大会
• [Presentation] The Dawn of Agriculture immunology (2016)
  • Author(s): Takahashi, H
  • Organizer: Lorentz Center Workshop
  • Place of Presentation: Lorentz Center, Leiden, The Netherlands
  • Year and Date: 2016-09-19
  • Int'l Joint Research / Invited
• [Presentation] R遺伝子による細胞死誘導の回避に寄与するキュウリモザイクウイルス外被タンパク質の1アミノ酸変異はウイルスの全身移行能を喪失させる (2016)
  • Author(s): 安藤由起・宮下脩平・安藤杉尋・高橋英樹
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山県、岡山市
  • Year and Date: 2016-03-22
• [Presentation] 次世代シークエンサーを用いたイネ有機栽培育苗培土の微生物多様性解析 (2015)
  • Author(s): 高橋英樹・松下裕子・伊藤豊彰・小林 隆・橋本知義・宮下脩平・守川俊幸・吉田重信・對馬誠也・安藤杉尋
  • Organizer: 平成27年度日本植物病理学会東北部会
  • Place of Presentation: 宮城県、仙台市
  • Year and Date: 2015-09-28
• [Presentation] 異なる有機栽培育苗土の微生物コミュニティーによるイネもみ枯細菌病抑制効果の比較 (2015)
  • Author(s): 安藤杉尋・鎗山純・小林隆・吉田重信・伊藤豊彰・宮下脩平・高橋英樹
  • Organizer: 平成27年度日本植物病理学会東北部会
  • Place of Presentation: 宮城県、仙台市
  • Year and Date: 2015-09-28
• [Presentation] トマトモザイクウイルスのゲノム／サブゲノム合成量比を制御するcisエレメント (2015)
  • Author(s): 宮下脩平・安藤杉尋・高橋英樹・石川雅之
  • Organizer: 平成27年度日本植物病理学会東北部会
  • Place of Presentation: 宮城県、仙台市
  • Year and Date: 2015-09-28
• [Presentation] Molecular basis of RCY1/RPP8 locus conferred dual resistance to Cucumber mosaic virus and Hyaloperonospora arabidopsidis. (2014)
  • Author(s): Takahashi, H., Sato, Y., Ando, Y. and Ando, S.
  • Organizer: 3rd Korea-Japan Joint Symposium on Plant Pathology
  • Place of Presentation: Busan, Korea
  • Year and Date: 2014-10-23 – 2014-10-24
  • Invited
• [Presentation] Analysis of pathogen-recognition domain in NB-LRR class RCY1/RPP8 locus conferring dual resistance to Cucumber mosaic virus and Hyaloperonospora arabidopsidis in Arabidopsis thaliana. (2014)
  • Author(s): Takahashi, H., Ando, Y. and Ando, S.
  • Organizer: XVI International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: Rhodos, Greece
  • Year and Date: 2014-07-06 – 2014-07-13
• [Presentation] Role of intron-mediated enhancement on accumulation of an Arabidopsis NB-LRR class R-protein that confers resistance to Cucumber mosaic virus. (2014)
  • Author(s): Sato, Y., Ando, S. and Takahashi, H.
  • Organizer: XVI International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: Rhodos, Greece
  • Year and Date: 2014-07-06 – 2014-07-13
• [Presentation] キュウリモザイクウイルス・アブラナ科べと病菌複合抵抗性を支配するRCY1/RPP8遺伝子座の組換えと病原体応答 (2014)
  • Author(s): 高橋英樹・安藤杉尋
  • Organizer: 平成26年度日本植物病理学会大会
  • Place of Presentation: 札幌市
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] キュウリモザイクウイルス抵抗性遺伝子RCY1に対するウイルス非病原性因子の機能ドメイン解析 (2014)
  • Author(s): 安藤由起・安藤杉尋・高橋英樹
  • Organizer: 平成26年度日本植物病理学会大会
  • Place of Presentation: 札幌市
  • Year and Date: 2014-06-04 – 2014-06-06
• [Book] Antiviral Resistance in Plants: Methods and Protocols, In Methods in Molecular Biology (Eds. Kappei Kobayashi and Masamichi Nishiguchi) (2019)
  • Author(s): Yukiyo Sato and Hideki Takahashi
  • Total Pages: 278
  • Publisher: Springer
• [Book] Cucumber Mosaic Virus (Eds. Peter Palukaitis and Fernando Garcia-Arenal) (2018)
  • Author(s): Chikara Masuta and Hideki Takahashi
  • Total Pages: 380
  • Publisher: APS Press
  • ISBN: 9780890546093