Mechanism for plant immune responses by WRKY transcription factors, physiological substrates of MAPKs

• Project/Area Number: 26292023
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Nagoya University
• Principal Investigator: Yoshioka Hirofumi 名古屋大学, 生命農学研究科, 准教授 (30240245)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000); Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 植物免疫 / シグナル伝達 / MAPキナーゼ / WRKY型転写因子

Outline of Final Research Achievements

Multiple WRKY transcription factors function in plant immunity through phosphorylation by MAPKs. Here, we show that plant immune WRKYs downregulate the large number of photosynthesis-related genes and inhibit photosynthetic CO2 fixation. This inhibition results in chloroplastic reactive oxygen species (ROS) burst in Nicotiana benthamiana epidermal cells. Sensing of Phytophthora infestans effector by a cognate receptor also induces the WRKY-dependent chloroplastic ROS burst in epidermal cells, conferring resistance to invasion of P. infestans into epidermal cells. These results suggest that the MAPK-WRKY pathway triggers epidermal cell-specific chloroplastic ROS burst to prevent pathogen invasion in N. benthamiana leaves.

Academic Significance and Societal Importance of the Research Achievements

植物が病原菌を認識して生体防御機構を始動すると、細胞死を伴った激しい免疫応答が誘導される。この誘導過程には、主にMAPキナーゼであるSIPKが関与する。しかし、その作用機構はほとんど明らかになっていない。本研究では、SIPKの基質であるWRKY型転写因子の下流遺伝子を網羅的に探索・比較することによって、植物免疫応答の制御機構を明らかにすることを目的とする。成果として、MAPK-WRKY系路がベンサミアナタバコ葉の表皮細胞において病原菌の侵入阻害を誘導することを明らかにした。

Research Products

• [Int'l Joint Research] LIPM(フランス)
• [Journal Article] An NADPH oxidase RBOH functions in rice roots during lysigenous aerenchyma formation under oxygen-deficient conditions. (2017)
  • Author(s): Yamauchi, T., Yoshioka, M., Fukazawa, A., Mori, H., Nishizawa, N.K., Tsutsumi, N., Yoshioka, H. and Nakazono, M.
  • Journal Title: The Plant Cell Volume: 印刷中 Issue: 4 Pages: 775-790
  • DOI: 10.1105/tpc.16.00976
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 植物免疫における活性酸素生成誘導のしくみ―植物はいかにして病原菌から身を護るのか？ (2017)
  • Author(s): 安達広明・吉岡博文
  • Journal Title: 化学と生物 Volume: 印刷中
  • NAID: 130007437458
• [Journal Article] SGT1 contributes to maintaining protein levels of MEK2DD to facilitate hypersensitive response-like cell death in Nicotiana benthamiana (2016)
  • Author(s): Kazuya Ichimura, Takehito Shinzato, Miki Edaki, Hirofumi Yoshioka, Ken Shirasu
  • Journal Title: Physiological and Molecular Plant Pathology Volume: 94 Pages: 47-52
  • DOI: 10.1016/j.pmpp.2016.04.001
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Hierarchical regulation of NADPH oxidase by protein kinases in plant immunity (2016)
  • Author(s): Yoshioka H., Adachi H., Nakano T., Miyagawa N., Asai S., Ishihama N., and Yoshioka M.
  • Journal Title: Physiological and Molecular Plant Pathology Volume: in press Pages: 20-26
  • DOI: 10.1016/j.pmpp.2016.03.004
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Nicotiana benthamiana MAPK-WRKY pathway confers resistance to a necrotrophic pathogen Botrytis cinerea. (2016)
  • Author(s): Adachi, H., Ishihama, N., Nakano, T., Yoshioka, M. and Yoshioka, H.
  • Journal Title: Plant Signaling & Behavior Volume: 11 Issue: 6 Pages: e1183085-e1183085
  • DOI: 10.1080/15592324.2016.1183085
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Nicotiana benthamiana MAPK-WRKY pathway confers resistance to a necrotrophic pathogen Botrytis cinerea. (2016)
  • Author(s): Adachi, H., Ishihama, N., Nakano, T., Yoshioka, M. and Yoshioka, H. (2016) Nicotiana benthamiana MAPK-WRKY pathway confers resistance to a necrotrophic pathogen Botrytis cinerea.
  • Journal Title: Plant Signal. Behav. Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A receptor pair with an integrated decoy converts pathogen disabling of transcription factors to immunity. (2015)
  • Author(s): Le Roux, C., Huet, G., Jauneau, A., Camborde, L., Tremousaygue, D., Kraut, A., Zhou, B., Levaillant, M., Adachi, H., Yoshioka, H., Raffaele, S., Berthome, R., Coute, Y., Parker, J.E. and Deslandes, L.
  • Journal Title: Cell Volume: 161 Issue: 5 Pages: 1074-1088
  • DOI: 10.1016/j.cell.2015.04.025
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cell death-inducing stresses are required for defense activation in DS1-phosphatidic acid phosphatase-silenced Nicotiana benthamiana. (2015)
  • Author(s): Masahito Nakano, Hirofumi Yoshioka, Kouhei Ohnishi, Yasufumi Hikichi, Akinori Kiba
  • Journal Title: Journal of Plant Physiology Volume: 184 Pages: 15-19
  • DOI: 10.1016/j.jplph.2015.06.007
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Molecular characterization of LjABCG1, an ATP-binding cassette protein in Lotus japonicus. (2015)
  • Author(s): Sugiyama, A., Fukuda, S., Takanashi, K., Yoshioka, M., Yoshioka, H., Narusaka, Y., Narusaka, M., Kojima, M., Sakakibara, H., Shitan, N., Sato, S., Tabata, S., Kawaguchi, M. and Yazaki, K.
  • Journal Title: PLoS ONE Volume: 10 Issue: 9 Pages: e0139127-e0139127
  • DOI: 10.1371/journal.pone.0139127
  • NAID: 120005715333
  • Peer Reviewed / Open Access
• [Journal Article] WRKY transcription factors phosphorylated by MAPK regulate a plant immune NADPH oxidase in Nicotiana benthamiana. (2015)
  • Author(s): Adachi, H., Nakano, T., Miyagawa, N., Ishihama, N., Yoshioka, M., Katou, Y., Yaeno, T., Shirasu, K. and Yoshioka, H.
  • Journal Title: Plant Cell Volume: 27 Issue: 9 Pages: 2645-2663
  • DOI: 10.1105/tpc.15.00213
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] リン酸化反応が制御するROSバーストの分子機構 (2015)
  • Author(s): 吉岡博文，安達広明，石濱伸明，中野孝明，白石佑太郎，宮川典子，野村裕也，吉岡美樹，浅井秀太
  • Journal Title: 日本植物病理学会報 Volume: 81 Pages: 1-8
  • NAID: 130005129474
  • Peer Reviewed
• [Journal Article] Kinase-mediated orchestration of NADPH oxidase in plant immunity. (2015)
  • Author(s): Hiroaki Adachi and Hirofumi Yoshioka
  • Journal Title: Brief. Funct. Genomics Volume: 14 Issue: 4 Pages: 253-259
  • DOI: 10.1093/bfgp/elv004
  • Peer Reviewed
• [Journal Article] An immune receptor decoy converts pathogen disabling of transcription factor-DNA binding into resistance (2015)
  • Author(s): Le Roux, C., Huet, G., Jauneau, A., Camborde, L., Trémousaygue, D., Kraut, A., Zhou, B., Levaillant, M., Adachi, H., Yoshioka, H., Rafaele, S., Berthomé, R., Couté, Y., Parker, J.E. and Deslandes, L.
  • Journal Title: Cell Volume: in press
  • Peer Reviewed
• [Presentation] Live-imaging of MAPK activity in plant immune responses (2017)
  • Author(s): 安達広明・石濱伸明・吉岡美樹・鳴坂真理・鳴坂義弘・吉岡博文
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学、鹿児島
  • Year and Date: 2017-03-16
• [Presentation] 植物免疫に関与するMAPキナーゼによる活性酸素生成機構 (2017)
  • Author(s): 吉岡博文
  • Organizer: 第４回新学術領域研究「酸素生物学」公開シンポジウム
  • Place of Presentation: 九州大学
  • Invited
• [Presentation] 免疫応答におけるSIPKの細胞内動態 (2016)
  • Author(s): 安達広明、波多江健太、吉岡美樹、鳴坂真理、鳴坂義弘、吉岡博文
  • Organizer: 平成28年度日本植物病理学会関西部会
  • Place of Presentation: 静岡県コンベンションアーツセンター，静岡
  • Year and Date: 2016-09-28
• [Presentation] Nicotiana benthamiana MAPK-WRKY pathway regulates effector-triggered ROS burst to confer resistance against Phytophthora infestans. (2016)
  • Author(s): Adachi, H., Nakano, T., Miyagawa, N., Ishihama, N., Yoshioka, M., Katou, Y., Yaeno, T., Shirasu, K. and Yoshioka, H.
  • Organizer: XVII International Congress on Molecular Plant-Microbe Interactions.
  • Place of Presentation: Portland
  • Year and Date: 2016-07-17
  • Int'l Joint Research
• [Presentation] 葉緑体による活性酸素生成を介した植物免疫機構.「活性酸素が支える生体防御力」 (2016)
  • Author(s): 吉岡博文・安達広明
  • Organizer: 第27回日本生体防御学会学術総会
  • Place of Presentation: 九州大学
  • Year and Date: 2016-07-07
  • Invited
• [Presentation] Protein kinases hierarchically regulate a plant immune NADPH oxidase (2016)
  • Author(s): Yoshioka, H
  • Organizer: The 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide
  • Place of Presentation: 仙台国際センター、仙台
  • Year and Date: 2016-05-20
  • Int'l Joint Research / Invited
• [Presentation] 植物免疫に関わるNADPH oxidaseの活性化機構 (2015)
  • Author(s): 吉岡博文
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] Protein kinase-mediated regulation of NADPH oxidase in plant immunity. (2015)
  • Author(s): Yoshioka, H., Adachi, H., Nakano, T., Miyagawa, N., Asai, S., Ishihama, N. and Yoshioka, M.
  • Organizer: 11th US-Japan Scientific Seminar
  • Place of Presentation: 香川
  • Year and Date: 2015-10-26
  • Int'l Joint Research / Invited
• [Presentation] MAPK-WRKY pathway up-regulates NbRBOHB gene and contributes to effector-triggered ROS burst in Nicotiana benthamiana. (2015)
  • Author(s): Adachi, H., Nakano, T., Miyagawa, N., Ishihama, N., Yoshioka, M., Katou, Y., Yaeno, T., Shirasu, K. and Yoshioka, H.
  • Organizer: The 4th International Conference on Biotic Plant Interactions.
  • Place of Presentation: 中国
  • Year and Date: 2015-08-01
  • Int'l Joint Research
• [Presentation] Transactivation of NbRBOHB gene via MAPK-WRKY pathway is essential for effector-triggered ROS burst in Nicotiana benthamiana. (2015)
  • Author(s): Adachi, H., Ishihama, N., Nakano, T., Miyagawa, N., Yoshioka, M., Yaeno, T., Shirasu, K. and Yoshioka, H.
  • Organizer: 12th International Conference on Reactive Oxygen and Nitrogen Species in Plants: from model systems to field.
  • Place of Presentation: イタリア
  • Year and Date: 2015-05-24
  • Int'l Joint Research
• [Presentation] RLCK-mediated phosphorylation of the N-terminus of RBOH regulates ROS burst in rice. (2015)
  • Author(s): Yoshioka, M., Yamaguchi, K., Fujiwara, M., Yoshimura, S., Kawasaki, T. and Yoshioka, H.
  • Organizer: 12th International Conference on Reactive Oxygen and Nitrogen Species in Plants: from model systems to field.
  • Place of Presentation: イタリア
  • Year and Date: 2015-05-24
  • Int'l Joint Research
• [Presentation] RNA-seqによるMAPK-WRKY経路下流の植物免疫関連遺伝子の探索 (2015)
  • Author(s): 安達広明・佐藤昌直・吉岡博文
  • Organizer: 平成27年度日本植物病理学会大会
  • Place of Presentation: 明治大学
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] RNA-seqによるMAPK-WRKY経路下流の免疫応答誘導機構の解析 (2015)
  • Author(s): 安達広明・佐藤昌直・吉岡博文
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2015-03-16 – 2015-03-31
• [Presentation] ナス科植物の免疫応答における活性酸素の生成機構 (2014)
  • Author(s): 吉岡博文
  • Organizer: 第11回日本ナス科コンソシアム年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2014-10-25 – 2014-10-26
  • Invited
• [Book] Methods in Molecular Biology (2014)
  • Author(s): Ishihama, N., Adachi, H., Yoshioka, M. and Yoshioka, H.
  • Total Pages: 11
  • Publisher: Springer/Humana Press
• [Remarks]
  • URL: http://www.agr.nagoya-u.ac.jp/%7ebio4283/index.html
• [Remarks]
  • URL: http://www.agr.nagoya-u.ac.jp/~bio4283/index.html
• [Remarks] 研究室ホームページ
  • URL: http://www.agr.nagoya-u.ac.jp/%7ebio4283/index.html