| Budget Amount *help |
¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
Nicotiana benthamiana is resistant to the potato blight pathogen, Phytophthora infestans. Screening using virus-induced gene silencing identified NbSAR8.2m, a secretory peptide, as an essential factor for the resistance. NbSAR8.2m-silenced plant is highly susceptible to P. infestans, leading systemic infection of the pathogen. In contrast, silencing of NbSAR8.2m showed no effect on the resistance to other pathogens, indicating that NbSAR8.2m is specifically involved in the resistance to P. infestans. While induction of cell death is impaired in NbSAR8.2m-silenced plants during the infection of P. infestans. Production of reactive oxygen species (ROS) was suppressed in SAR8.2m-silenced plants during the infection of P. infestans, but ROS production induced by elicitor was comparable between control and NbSAR8.2m-silenced plants. These results suggested that NbSAR8.2m may suppress the effectors activity of P. infestans.
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