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Molecular mechanism of chlorosis in plant virus diseases

Research Project

Project/Area Number 26292026
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Plant protection science
Research InstitutionEhime University

Principal Investigator

KOBAYASHI Kappei  愛媛大学, 農学研究科, 教授 (40244587)

Co-Investigator(Kenkyū-buntansha) 舘田 知佳  公益財団法人岩手生物工学研究センター, 園芸資源研究部, 研究員 (30774111)
関根 健太郎  琉球大学, 農学部, 准教授 (30574058)
Co-Investigator(Renkei-kenkyūsha) MOCHIZUKI Tomofumi (ATSUMI Go)  大阪府立大学, 生命環境科学研究科, 講師 (30469837)
Research Collaborator WALIULLAH Sumyya  
BHOR Sachin Ashok  
AKHTER Md. Shamim  
KOSAKA Naomi  
SUGANUMA Yusuke  
SUGIWAKA Yuji  
TAKAOKA Koichi  
TERADA Shinobu  
NISIOKA Keiji  
TADANI Moeka  
NAKAMURA Mizuki  
ISLAM Shaikhul  
SAKAMOTO Hikaru  
TANAKA Keisuke  
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywords植物ウイルス病 / 遺伝子組換え植物 / 遺伝子発現解析 / 病害応答 / 葉緑体 / 発症機構 / 防御応答 / 葉緑体障害 / 植物 / ウイルス / 発病 / 葉緑体タンパク質 / 感染症 / 植物病害 / 退緑
Outline of Final Research Achievements

The loss of green pigment (chlorosis) or yellowing in the leaves are the common symptoms of plant virus diseases. To elucidate which genes or proteins are involved in the development of such chlorosis or yellowing symptoms, we generated five genetically modified plants, in which chlorosis or yellowing symptoms are induced by the treatment with a chemical that does not affect plants by itself. Studies using these plants have suggested that plants' functions responsible for the defense against pathogens including viruses are also involved in the development of chlorosis or yellowing symptoms.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (29 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (13 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 13 results,  Open Access: 5 results,  Acknowledgement Compliant: 11 results) Presentation (15 results) (of which Invited: 2 results)

  • [Int'l Joint Research] Universite de Sherbrooke(カナダ)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Inducible expression of magnesium protoporphyrin chelatase subunit I (CHLI)-amiRNA provides insights into Cucumber mosaic virus Y satellite RNA-induced chlorosis symptoms2017

    • Author(s)
      Bhor S. A., Tateda C., Mochizuki T., Sekine K.-T., Yaeno T., Yamaoka N., Nishiguchi M. and Kobayashi K.
    • Journal Title

      VirusDisease

      Volume: 28 Issue: 1 Pages: 69-80

    • DOI

      10.1007/s13337-017-0360-1

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Inducible transgenic tobacco system to study the mechanisms underlying chlorosis mediated by the silencing of chloroplast heat shock protein 902017

    • Author(s)
      Bhor S. A., Tateda C., Mochizuki T., Sekine K.-T., Yaeno T., Yamaoka N., Nishiguchi M. and Kobayashi K.
    • Journal Title

      VirusDisease

      Volume: 28 Issue: 1 Pages: 81-92

    • DOI

      10.1007/s13337-017-0361-0

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Simple and Quantitative Detection of Apple latent spherical virus Vector by a Spot Hybridization2016

    • Author(s)
      Bhor S. A., Akhter M. S., Yaeno T., Yamaoka N., Nishiguchi M., Kaido M. and Kobayashi K.
    • Journal Title

      International Journal of Modern Botany

      Volume: 6 Pages: 31-36

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Review of Beet pseudoyellows virus genome structure built the consensus genome organization of cucumber strains and highlighted the unique feature of strawberry strain2016

    • Author(s)
      Akhter M. S., Bhor S. A., Hlalele N., Nao M., Sekine K.-T., Yaeno T., Yamaoka N., Nishiguchi M., Gubba A. and Kobayashi K.
    • Journal Title

      Virus Genes

      Volume: 52 Issue: 6 Pages: 828-834

    • DOI

      10.1007/s11262-016-1376-0

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Hammer blot-mediated RNA extraction: an inexpensive, labor-saving method to extract RNA for plant virus detection2016

    • Author(s)
      Akhter M. S., Yaeno T, Yamaoka N., Nishiguchi M. and Kobayashi K.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 82 Issue: 5 Pages: 268-272

    • DOI

      10.1007/s10327-016-0673-8

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Analysis of rice RNA-dependent RNA polymerase (OsRDR6) gene in response to viral, bacterial and fungal pathogens2016

    • Author(s)
      Wagh S. G., Alam M. M., Kobayashi K., Yaeno T., Yamaoka N., Toriba T., Hirano H.-Y. and Nishiguchi M.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 82 Issue: 1 Pages: 12-17

    • DOI

      10.1007/s10327-015-0630-y

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Rice necrosis mosaic virus, a fungal transmitted Bymovirus: complete nucleotide sequence of the genomic RNAs and subgrouping of bymoviruses.2016

    • Author(s)
      Wagh S. G., Kobayashi K., Yaeno T., Yamaoka N. and Nishiguchi M.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 82 Issue: 1 Pages: 38-42

    • DOI

      10.1007/s10327-015-0618-7

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Conferring high-temperature tolerance to nontransgenic tomato scions using graft transmission of RNA silencing of the fatty acid desaturase gene.2016

    • Author(s)
      Nakamura S., Hondo K., Kawara T., Okazaki Y., Saito K., Kobayashi K., Yaeno T., Yamaoka N. and Nishiguchi M.
    • Journal Title

      Plant Biotech Journal

      Volume: 14 Issue: 2 Pages: 783-790

    • DOI

      10.1111/pbi.12429

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Combined DECS Analysis and Next-Generation Sequencing Enable Efficient Detection of Novel Plant RNA Viruses.2016

    • Author(s)
      Yanagisawa H., Tomita R., Katsu K., Uehara T., Atsumi G., Tateda C., Kobayashi K. and Sekine K.-T.
    • Journal Title

      Viruses

      Volume: 8 Issue: 3 Pages: 70-70

    • DOI

      10.3390/v8030070

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The chloroplastic protein THF1 interacts with the coiled-coil domain of the disease resistance protein N' and regulates light-dependent cell death.2016

    • Author(s)
      Hamel L.-P., Sekine K.-T., Wallon T., Sugiwaka Y., Kobayashi K., and Moffett P.
    • Journal Title

      Plant Physiology

      Volume: 170 Issue: 1 Pages: 658-674

    • DOI

      10.1104/pp.16.00234

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Molecular epidemiology of Impatiens necrotic spot virus on greenhouse ornamental plants in a local area of Japan.2015

    • Author(s)
      Nekoduka, S., Kobayashi, K., Fuji, S.-i. and Sano, T.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 81 Issue: 6 Pages: 429-438

    • DOI

      10.1007/s10327-015-0615-x

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cauliflower mosaic virus Tav protein induces leaf chlorosis in transgenic tobacco through a host response to its virulence function2015

    • Author(s)
      60)Waliullah, S., Kosaka, N., Yaeno, T., Ali, M. E., Sekine, K.-T., Atsumi, G., Yamaoka, N., Nishiguchi, M., Takahashi, H. and Kobayashi, K.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 印刷中

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Artificial induction of a plant virus protein in transgenic tobacco provides a synchronous system for analyzing the process of leaf chlorosis2014

    • Author(s)
      S. Waliullah, T. Mochizuki, K.-T. Sekine, G. Atsumi , M. E. Ali, T. Yaeno, N. Yamaoka, M. Nishiguchi and K. Kobayashi.
    • Journal Title

      Physiol Mol Plant Pathol

      Volume: 88 Pages: 43-51

    • DOI

      10.1016/j.pmpp.2014.08.005

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Upregulation of pathogenesis-related protein genes after the induced silencing of chloroplast protein genes in transgenic tobacco2017

    • Author(s)
      Bhor, S.A., Tateda, C., Mochizuki, T., Sekine, K.-T., Yaeno, T., Yamaoka, N., Nishiguchi, M. and Kobayashi, K.
    • Organizer
      平成29年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 誘導型カリフラワーモザイクウイルスTav遺伝子発現シロイヌナズナが示す成長抑制における遺伝子発現の網羅的解析2017

    • Author(s)
      寺田忍,Bhor Sachin Ashok,田中啓介,坂本光,関根健太郎,八丈野孝,山岡直人,西口正通,小林括平
    • Organizer
      平成29年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] カリフラワーモザイクウイルスTav遺伝子発現シロイヌナズナにおける細胞自律的な成長抑制2017

    • Author(s)
      寺田忍,田中啓介,坂本光,関根健太郎,八丈野孝,山岡直人,小林括平
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] トランスジェニックモデル植物を用いた植物ウイルス病発症機構の解析2017

    • Author(s)
      寺田忍,Bhor Sachin Ashok,望月知史,舘田知佳,関根健太郎,田谷萌香,Islam Shaikhul,中村瑞生,三好沙季,八丈野孝,小林括平
    • Organizer
      日本植物病理学会植物ウイルス病研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] The chloroplast protein THF1 interacts with the cytosolic protein TIP32016

    • Author(s)
      Keiji Nishioka, Yuji Sugiwaka, Kappei Kobayashi
    • Organizer
      第14回松山国際学術 シンポジウム,および同若手の会
    • Place of Presentation
      愛媛大学(愛媛県松山市)
    • Year and Date
      2016-09-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] Artificial induction of chlorosis symptom through the silencing of chloroplast genes in transgenic tobacco2016

    • Author(s)
      Bhor, S.A., Sekine, K.-T., Yaeno, T., Yamaoka, N., Nishiguchi, M. and Kobayashi, K
    • Organizer
      平成28年度日本植物病理学会大会
    • Place of Presentation
      岡山コンベンションセンター(岡山県岡山市)
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] Functional analysis of Beet pseudo-yellows virus RNA1 encoded-p6 gene2016

    • Author(s)
      Akhter, M.S., Bhor S.A., Yaeno, T., Yamaoka, N., Nishiguchi, M. and Kobayashi, K.
    • Organizer
      平成28年度日本植物病理学会大会
    • Place of Presentation
      岡山コンベンションセンター(岡山県岡山市)
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] Construction of a single copy agroinfection vector for the development of infectious cDNA clone of plant viruses2015

    • Author(s)
      Akhter, M.S., Sekine, K.-T., Yaeno, T., Yamaoka, N., Nishiguchi, M. and Kobayashi, K.
    • Organizer
      平成27年度日本植物病理学会関西部会
    • Place of Presentation
      あわぎんホール(徳島県徳島市)
    • Year and Date
      2015-09-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 薬剤誘導型カリフラワーモザイクウイルスTav遺伝子導入シロイヌナズナは誘導薬剤依存的に葉の黄化および成長抑制を示す2015

    • Author(s)
      寺田忍・Bhor Sachin Ashok・関根健太郎1・八丈野孝・山岡直人・西口正通・小林括平
    • Organizer
      平成27年度日本植物病理学会関西部会
    • Place of Presentation
      あわぎんホール(徳島県徳島市)
    • Year and Date
      2015-09-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 葉緑体における活性酸素種消去系大量発現のための葉緑体形質転換法の確立2015

    • Author(s)
      小坂奈央美・八丈野孝・山岡直人・西口正通・小林括平
    • Organizer
      平成27年度植物感染生理談話会
    • Place of Presentation
      メルパルク松山(愛媛県松山市)
    • Year and Date
      2015-08-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] 薬剤誘導型カリフラワーモザイクウイルスTav遺伝子導入シロイヌナズナは誘導薬剤依存的に葉の黄化および成長抑制を示す2015

    • Author(s)
      寺田忍・Bhor Sachin Ashok・関根健太郎・八丈野孝・山岡直人・西口正通・小林括平
    • Organizer
      平成27年度植物感染生理談話会
    • Place of Presentation
      メルパルク松山(愛媛県松山市)
    • Year and Date
      2015-08-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] Cauliflower mosaic virus Tav protein induces leaf chlorosis in transgenic tobacco through an SA-independent host response to its virulence function2015

    • Author(s)
      Waliullah, S., Kosaka, N., Yaeno, T., Ali, M. E., Sekine, K.-T., Atsumi, G., Yamaoka, N., Nishiguchi, M. Takahashi, H. and Kobayashi, K.
    • Organizer
      日本植物病理学会平成27年度大会
    • Place of Presentation
      明治大学
    • Year and Date
      2015-03-29 – 2015-03-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] 葉緑体タンパク質THF1の新規相互作用因子の探索2015

    • Author(s)
      杉若侑冶・田島薫・菅沼裕介・八丈野孝・山岡直人・西口正通・小林括平
    • Organizer
      日本植物病理学会平成27年度大会
    • Place of Presentation
      明治大学
    • Year and Date
      2015-03-29 – 2015-03-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] Exploration of mechanisms for plant virus symptom expression --- a challenge for plant protection in future2014

    • Author(s)
      KOBAYASHI K, WALIULLAH S, BHOR SA, AKHTER MS, KOSAKA N, SUGANUMA Y, SUGIWAKA Y, TAJIMA K, YAMASHITA M, TOMITA R, ATSUMI G and SEKINE K-T
    • Organizer
      XXIII National Conference of Indian Virological Society
    • Place of Presentation
      Tamil Nadu Agricultural University, Coimbatore (India)
    • Year and Date
      2014-12-18 – 2014-12-20
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Establishment of virus-induced gene silencing (VIGS) for functional analysis of endogenous genes in Nicotiana tabacum2014

    • Author(s)
      BHOR SA and KOBAYASHI K
    • Organizer
      XXIII National Conference of Indian Virological Society
    • Place of Presentation
      Tamil Nadu Agricultural University, Coimbatore (India)
    • Year and Date
      2014-12-18 – 2014-12-20
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2019-03-29  

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