• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The study of the interaction between Magnaportha effector AVR-Pik and rice HMA domain protein

Research Project

Project/Area Number 26292027
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Plant protection science
Research InstitutionIwate Biotechnology Research Center

Principal Investigator

Kanzaki Hiroyuki  公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 研究専門員 (10390882)

Co-Investigator(Renkei-kenkyūsha) TERAUCHI Ryohei  公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 研究部長 (50236981)
SAITOH Hiromasa  公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 主任研究員 (20414336)
FUJISAKI Koki  公益財団法人岩手生物工学研究センター, 園芸資源研究部, 主任研究員 (30626510)
TAKEDA Takumi  公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 主任研究員 (80423036)
Research Collaborator ITO Kazue  公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 研究助手
KANZAKI Eiko  公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 研究技術員
Kamoun Sophien  The Sainsbury Laboratory, Professor
Banfield Mark  John Innes Centre, Department of Biological Chemistry, Professor
Kroj Thomas  INRA, UMR, BGPI, Professor
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
KeywordsHMAドメイン / エフェクター遺伝子 / いもち病抵抗性遺伝子 / 活性酸素種 / 罹病性 / 酵母2ハイブリッド法 / イネ / いもち病菌 / ROS / いもち病菌エフェクター / 罹病性遺伝子 / いもち病菌エフェクターAVR-Pik / sHMA / エフェクター / AVR-Pik / タンパク質相互作用
Outline of Final Research Achievements

The rice R-gene protein Pik-1 and RGA5 have an integrated Heavy Metal-Associated (HMA) domain that directly binds secreted effector proteins from the rice blast fungus Magnaporthe oryzae. The HMA domain is also found indefines a family of small proteins of rice designated as small HMA-containing proteins (sHMAs). We discovered that the M. oryzae cytoplasmic effector AVR-Pik-D binds six members of the sHMA family, and stabilizes sHMA1 in planta. Knockdown of sHMA genes in rice enhanced infection by a compatible isolate of M. oryzae, indicating that sHMAs are required for rice susceptibility to blast infection, and can therefore be considered as susceptibility (S-) genes. sHMA proteins negatively regulate the accumulation of reactive oxygen species (ROS), potentially by interacting with a protein that scavenges ROS. Our findings led us to propose an evolutionary model in which M. oryzae evolved the AVR-Pik effector to bind and stabilize sHMA proteins and increase host susceptibility.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (13 results)

All 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 7 results,  Open Access: 7 results,  Acknowledgement Compliant: 7 results) Presentation (2 results) Book (2 results)

  • [Int'l Joint Research] John Innes Centre (Norwich)/The Sainsbury Laboratory (Norwich)(英国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] INRA (Monpellier)(フランス)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] A chloroplast-localized protein LESION AND LAMINA BENDING affects defense and growth responses in rice.2016

    • Author(s)
      Tamiru, M., Takagi, H., Abe, A., Yokota, T., Kanzaki, H., Okamoto, H., Saitoh, H., Takahashi, H., Fujisaki, K., Oikawa, K., Uemura, A., Natsume, S., Jikumaru, Y., Matsuura, H., Umemura, K., Terry, M. J., Terauchi, R.
    • Journal Title

      New Phytologist

      Volume: 210 Issue: 4 Pages: 1282-1297

    • DOI

      10.1111/nph.13864

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Whole genome sequencing approaches to understand Magnaporthe-rice interactions.2016

    • Author(s)
      Terauchi, R., Kanzaki, H., Fuijsaki, K., Takagi, H., Abe, A., Yoshida, K., Okuyama, Y., Tamiru, M., Saitoh, H.
    • Journal Title

      Physiological and Molecular Plant Pathology

      Volume: 94 Pages: 1-5

    • DOI

      10.1016/j.pmpp.2016.03.007

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Rice Exo70 interacts with a fungal effector, AVR-Pii and is required for AVR-Pii-triggered immunity2015

    • Author(s)
      Fujisaki, K., Abe, Y., Ito, A., Saitoh, H., Yoshida, K., Kanzaki H., Kanzaki, E., Utsushi, H., Yamashita, T., Kamoun, S. and Terauchi, R.
    • Journal Title

      The Plant Journal

      Volume: 83 Issue: 5 Pages: 875-887

    • DOI

      10.1111/tpj.12934

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Structural basis of pathogen recognition by an integrated HMA domain in a plant NLR immune receptor.2015

    • Author(s)
      Maqbool, A., Saitoh, H., Franceschetti, M., Stevenson, C.E.M., Uemura, A., Kanzakii, H., Kamoun, S., Terauchi, R., Banfield, M. J.
    • Journal Title

      eLife

      Volume: 4 Pages: 1-24

    • DOI

      10.7554/elife.08709

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] MutMap accelerates breeding of a salt-tolerant rice cultivar2015

    • Author(s)
      Takagi, H., Tamiru, M., Abe, A., Yoshida, K., Uemura, A., Yaegashi, H., Obara, T., Oikawa, K., Utsushi, H., Kanzaki, E., Mitsuoka, C., Natsume, S., Kosugi, S., Kanzaki, H., Matsumura, H., Urasaki, N., Kamoun, S., Terauchi, R.
    • Journal Title

      Nature Biotechnology

      Volume: 33 Issue: 5 Pages: 445-449

    • DOI

      10.1038/nbt.3188

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The rice (Oryza saliva L.) LESION MIMIC RESEMBLING, which encodes an AAA-type ATPase, is implicated in defense response2014

    • Author(s)
      Fekih, R., Tamiru, M., Kanzaki, H., Abe, A., Yoshida, K., Kanzaki, E., Saitoh, H., Takagi, H., Natsume, S., Undan, J. R., Undan, J, Terauchi, R.
    • Journal Title

      Molecular Genetics and Genomics

      Volume: 290 Issue: 2 Pages: 611-622

    • DOI

      10.1007/s00438-014-0944-z

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The NB-LRR proteins RGA4 and RGA5 interact functionally and physically to confer disease resistance2014

    • Author(s)
      Cesari, S., Kanzaki, H., Fujiwara, T., Bernoux, M., Chalvon, V., Kawano, Y., Shimamoto, K., Dodds, P., Terauchi, R., Kroj, T.
    • Journal Title

      EMBO Journal

      Volume: 33 Issue: 17 Pages: 1941-1959

    • DOI

      10.15252/embj.201487923

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] イネ罹病性遺伝子sHMAの機能解析2016

    • Author(s)
      神崎洋之、伊藤和江、三岡周子、神崎英子、藤崎恒喜、竹田匠、斉藤宏昌、Sophien Kamoun、寺内良平
    • Organizer
      日本育種学会
    • Place of Presentation
      岡山コンベンションセンター
    • Year and Date
      2016-09-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] いもち病菌(Magnaporthe oryzae)非病原力遺伝子AVR-Pikと相互作用するイネHMA domainタンパク質の機能解析2014

    • Author(s)
      神崎洋之、斉藤宏昌、藤崎恒喜、小林光智衣、伊藤和江、三岡周子、神崎英子、Mark Banfield, Sophien Kamoun,寺内良平
    • Organizer
      平成26年度日本植物病理学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-06-02 – 2014-06-04
    • Related Report
      2014 Annual Research Report
  • [Book] Chapter 3 (Whole Genome Sequencing to Identify Genes and QTL in Rice) in Advances in the Understanding of Biological Sciences Using Next Generation Sequencing (NGS) Approaches2015

    • Author(s)
      Terauchi R., Abe A., Takagi H., Tamiru M., Fekih R., Natsume S., Yaegashi H., Kosugi S., Kanzaki H., Matsumura H., Saitoh H., Yoshida K., Cano L., Kamoun S.
    • Publisher
      Springer
    • Related Report
      2015 Annual Research Report
  • [Book] Methods in Molecular Biology, Vol. 1127, Chapter 202014

    • Author(s)
      Kanzaki, H., Yoshida, K., Saitoh, H., Tamiru, M. and Terauchi, R.
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2023-03-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi