| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Outline of Final Research Achievements |
To contribute the development of a novel mite management technology using UVB radiation in greenhouse, we demonstrated 1) molecular mechanisms of UV damage and 2) protective function by antioxidants. With regard to 1), the interrelation between the accumulation of DNA lesion and mortality and photoreactivation were proven. Larvae exposed to UVB radiation died mostly at their protochrysalis stages. Structural protein genes were downregulated in damaged individuals at protochrysalis stage. We also found that low temperature enhanced control effects of UVB irradiation on spider mites. In 2), carotenoids contained in spider mite bodies were identified, and its protective function preventing from lipid peroxide accumulation was demonstrated. Moreover, it was demonstrated that a phytoseiid mite (natural enemy) received the protective effects by antioxidant compounds through pollen diet.
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