Identification of novel plant defense activators based on ROS signaling and membrane trafficking
Project/Area Number |
26292032
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Plant protection science
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Research Institution | Tokyo University of Science |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
来須 孝光 東京工科大学, 公私立大学の部局等, 助教 (50422499)
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Co-Investigator(Renkei-kenkyūsha) |
KITAHATA Nobutaka 東京理科大学, 理工学部, 助教 (10435646)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
|
Keywords | 感染防御応答 / サリチル酸 / 細胞内膜交通系 / 植物免疫活性化剤 / 抵抗性誘導剤 / 活性酸素種 / エンドソーム / 液胞 / カルシウムイオン / 植物免疫 / ハイスループットスクリーニング / カロース / 膜交通系 / 植物防除剤 / エンドサイトーシス / NADPHオキシダーゼ / PI3キナーゼ |
Outline of Final Research Achievements |
Based on the correlation between the temporal pattern of NADPH oxidase/Rboh-mediated production of reactive oxygen species (ROS) and downstream defense responses including hypersensitive cell death, we established a high-throughput screening to identify novel compounds that modulate plant defense responses. From a total of 11,000 compounds screened, we identified compounds that activate ROS production and hypersensitive cell death in BY-2 cells. We report the effects of these chemicals on the MAMP-induced various responses in Arabidopsis including expression of various defense-related genes in planta. We also conducted comparative transcriptomic analysis using RNA-seq to reveal the effects of the chemicals on MAMP-induced gene expression. Results suggest that some chemicals activate different defense signaling pathways distinct from the known plant defense activators that activate the salicylic acid pathway.
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Report
(4 results)
Research Products
(217 results)
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[Journal Article] Autophagy supports biomass production and nitrogen use efficiency at the vegetative stage in rice2015
Author(s)
Wada S, Hayashida Y, Izumi M, Kurusu T, Hanamata S, Kanno K, Kojima S, Yamaya T, Kuchitsu K, Makino A, Ishida H
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Journal Title
Plant Physiology
Volume: in press
Issue: 1
Pages: 60-73
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Autophagy supports biomass production and nitrogen use elliciency at the vegetative stage in rice.2015
Author(s)
Wada S, Hayashida Y, Izumi M, Kurusu T, Hanamata S, Kanno K, Kojima S,Yamaya T, Kuchitsu K, ~fakino A, Ishida II
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Journal Title
Plant Physiology
Volume: 印刷中
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Peer Reviewed
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[Presentation] 植物免疫活性化剤の探索と作用機構の解析2015
Author(s)
北畑信隆, 大滝幹, 吉川岳史, 助川夏雄, 吉田亜祐美,来須孝光, 浅見忠男, 朽津和幸
Organizer
日本農薬学会
Place of Presentation
玉川大学(東京都)
Year and Date
2015-03-18 – 2015-03-20
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[Presentation] イネの感染防御応答及び脂質代謝におけるオートファジーの役割の解析2014
Author(s)
二平耕太朗、来須孝光、高橋章夫、岡咲洋三、安藤大智、佐谷南海子、北畑信隆、花俣繁、小嶋美紀子、榊原均、斉藤和季、朽津和幸
Organizer
東京理科大学総合研究機構 RNA科学総合研究センター公開シンポジウム 「RNA科学のこれから―その可能性と展望」
Place of Presentation
東京理科大学葛飾キャンパス・図書館大ホール(東京都)
Year and Date
2014-12-22
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[Presentation] 新規植物免疫活性化剤候補化合物の選抜と作用機構の解析2014
Author(s)
吉川岳史、北畑信隆、八木智華子、来須孝光、浅見忠男、朽津和幸
Organizer
東京理科大学総合研究機構 RNA科学総合研究センター公開シンポジウム 「RNA科学のこれから―その可能性と展望」
Place of Presentation
東京理科大学葛飾キャンパス・図書館大ホール(東京都)
Year and Date
2014-12-22
Related Report
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[Presentation] 植物の実りのイメージング2014
Author(s)
朽津 和幸
Organizer
イメージングフロンティアシンポジウム2014
Place of Presentation
東京理科大学野田キャンパス(千葉県)
Year and Date
2014-12-20
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Invited
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[Book] Peptide Science 20142015
Author(s)
Nara M, Miyakawa T, Tanokura M, Kuchitsu K, Shimizu T, Morii H
Total Pages
369
Publisher
Protein Research Foundation.
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