| Project/Area Number |
26292034
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Hokkaido University |
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Principal Investigator |
Ezawa Tatsuhiro 北海道大学, (連合)農学研究科(研究院), 准教授 (40273213)
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| Co-Investigator(Kenkyū-buntansha) |
増田 税 北海道大学, (連合)農学研究科(研究院), 教授 (60281854)
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| Co-Investigator(Renkei-kenkyūsha) |
SAITO Katsuharu 信州大学, 大学院農学研究院, 教授 (40444244)
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| Research Collaborator |
MARUYAMA Hayato 北海道大学, 大学院農学研究院, 助教
KAWAHARA Ai 北海道大学, 大学院農学院
KIKUCHI Yusuke 北海道大学, 大学院農学院
YOKOYAMA Kaede 北海道大学, 大学院農学院
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Keywords | アーバスキュラー菌根菌 / 遺伝子サイレンシング / 共生 / 植物ウィルスベクター / リン酸 |
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| Outline of Final Research Achievements |
Arbuscular mycorrhizal fungi associate with more than 80% of land plants, deliver phosphate to the host, and, in return, receive photosynthate from the host. The molecular mechanism underlying the symbiotic phosphate delivery, however, has yet to be elucidated. For the first time, we have established a gene silencing technique in the fungi using plant viral vector (i.e. virus-induced gene silencing) in this study. With this technique, we demonstrated that a fungal aquaporin mediates long-distance phosphate translocation through hyphae, which is driven by water flow created primarily by transpiration. A phosphate exporter responsible for phosphate transfer from the fungal cell to plant cell is also identified and characterized in this study.
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