Comprehensive analysis of archaeal transcriptional regulators in a hyperthermophile

• Project/Area Number: 26292038
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Kyoto University
• Principal Investigator: Kanai Tamotsu 京都大学, 工学研究科, 講師 (10346083)
• Co-Investigator(Renkei-kenkyūsha): ATOMI Haruyuki 京都大学, 大学院工学研究科, 教授 (90243047)
• Research Collaborator: YOSHIDA Ko ; KANESEKI Tsuyoshi ; TANIMOTO Mitsuru ; ASLAM Mehwish ; GUNJI Ryoma ; YAMADA Masahiro
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2016: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: アーキア / 転写制御因子 / 超好熱菌 / 転写 / 微生物 / 芳香族アミノ酸

Outline of Final Research Achievements

We have identified a transcriptional repressors for gluconeogenic enzymes and intracellular polysaccharide synthase in the hyperthermophilic archaeon, Thermococcus kodakarensis. A transcriptional activator, Tar, may control the entire aromatic amino acid biosynthesis systems in this organism. A collaborative research with Ehime University conducted a three dimensional structural analysis of Tar. It was suggested that the novel Lrp/AsnC family transcriptional repressor is involved in the control of a asparaginase gene, with a possibility of a dynamic binding/dissociation mechanism with DNA. By genetic recombination of the transcriptional regulatory system of T. kodakarensis, strains which produce hydrogen with higher efficiency were developed.

Research Products

• [Journal Article] Gene regulation of two ferredoxin:NADP+ oxidoreductases by the redox-responsive regulator SurR in Thermococcus kodakarensis. (2017)
  • Author(s): Hidese R, Yamashita K, Kawazuma K, Kanai T, Atomi H, Imanaka T, Fujiwara S.
  • Journal Title: Extremophiles Volume: 21 Issue: 5 Pages: 903-917
  • DOI: 10.1007/s00792-017-0952-0
  • Peer Reviewed
• [Journal Article] Engineering of a hyperthermophilic archaeon, Thermococcus kodakarensis, that displays chitin-dependent hydrogen production (2017)
  • Author(s): Aslam M, Horiuchi A, Simons JR, Jha S, Yamada M, Odani T, Fujimoto R, Yamamoto Y, Gunji R, Imanaka T, Kanai T, Atomi H.
  • Journal Title: Appl Environ Microbiol. Volume: 83 Issue: 15
  • DOI: 10.1128/aem.00280-17
  • Peer Reviewed
• [Journal Article] Genetic analyses of the functions of [NiFe]-hydrogenase maturation endopeptidases in the hyperthermophilic archaeon Thermococcus kodakarensis (2017)
  • Author(s): Tamotsu Kanai, Ayako Yasukochi, Jan Robert Simons, Joseph Walker Scott, Wakao Fukuda, Tadayuki Imanaka and Haruyuki Atomi
  • Journal Title: Extremophiles Volume: 21 Issue: 1 Pages: 27-39
  • DOI: 10.1007/s00792-016-0875-1
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Overproduction of the membrane-bound [NiFe]-hydrogenase in Thermococcus kodakarensis and its effect on hydrogen production. (2015)
  • Author(s): Kanai T, Simons JR, Tsukamoto R, Nakajima A, Omori Y, Matsuoka R, Beppu H, Imanaka T, Atomi H.
  • Journal Title: Front. Microbiol. Volume: 26 Pages: 847-847
  • DOI: 10.3389/fmicb.2015.00847
  • NAID: 120005848243
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The X-ray crystal structure of the euryarchaeal RNA polymerase in an open clamp configuration (2014)
  • Author(s): Sung-Hoon Jun, Akira Hirata*, Tamotsu Kanai, Thomas J. Santangelo, Tadayuki Imanaka and Katsuhiko S. Murakami*
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 5132-5132
  • DOI: 10.1038/ncomms6132
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Different roles of two transcription factor B proteins in the hyperthermophilic archaeon Thermococcus kodakarensis (2014)
  • Author(s): Hidese, R., Nishikawa, R., Gao, L., Katano, M., Imai, T. Kato, S., Kanai, T., Atomi, H., Imanaka, T., Fujiwara, S.
  • Journal Title: Extremophiles (Springer Co.Ltd., New York) Volume: 18 Issue: 3 Pages: 573-588
  • DOI: 10.1007/s00792-014-0638-9
  • Peer Reviewed
• [Presentation] 超好熱性アーキアThermococcus kodakarensisにおける 新規Lrp/AsnC型転写制御因子の機能解明 (2017)
  • Author(s): 谷本 充、吉田 晃、山本 康之、金井 保、跡見 晴幸
  • Organizer: 極限環境生物学会
• [Presentation] 芳香族アミノ酸の生合成遺伝子群の発現を制御するアーキア転写活性化因子(Tar)の分子認識機構の解明 (2017)
  • Author(s): 臼井 孝典、金井 保、跡見 晴幸、堀 弘幸1、平田 章
  • Organizer: 極限環境生物学会
• [Presentation] アーキアRNAポリメラーゼの転写活性化型開始複合体の構造解析に向けての準備状況 (2017)
  • Author(s): 小西 智也、金井 保、跡見 晴幸、堀 弘幸、平田 章
  • Organizer: 極限環境生物学会
• [Presentation] アーキアにおけるアミノ酸生合成関連遺伝子の発現制御機構 (2016)
  • Author(s): 跡見晴幸、金関剛史、山本康之、今中忠行、金井保
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-27
  • Invited
• [Presentation] 超好熱性アーキアにおける新規転写制御因子の同定 (2014)
  • Author(s): 跡見晴幸、山本康之、吉田晃、金関剛史、今中忠行、金井保
  • Organizer: 第37回分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-26
  • Invited
• [Presentation] 超好熱性アーキアThermococcus kodakarensisにおける 芳香族アミノ酸生合成経路の転写制御機構の解明 (2014)
  • Author(s): 金関 剛史, 山本 康之, 今中忠行, 金井 保, 跡見 晴幸
  • Organizer: 第66回日本生物工学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] Genome-wide screening to identify novel transcriptional regulators from a hyperthermophilic archaeon, Thermococcus kodakarensis (2014)
  • Author(s): Tamotsu Kanai, Yasuyuki Yamamoto, Tsuyoshi Kaneseki, Tadayuki Imanaka and Haruyuki Atomi
  • Organizer: 10th International Congress on Extremophiles
  • Place of Presentation: St. Petersburg, Russia
  • Year and Date: 2014-09-08