| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
|
|---|
| Outline of Final Research Achievements |
The structure of nukacin ISK-1 was found to have two states chemically exchanged by solution NMR. The exchange rate kex was about 1.5 s-1. The high abundance ratio had high affinity to the cell wall peptidoglycan precursor lipid II. The NMR-based model of nukacine ISK-1 in a complex with lipid II indicated that the hydrophobic amino acid side chain in the Ring C region of nukacin ISK-1 participates in binding to the lipid II fat chain region, and the region near Ring A participates in binding with the phosphate group of lipid II. By attaching the leader peptide of the precursor peptide of nukacin ISK-1, the unusual amino acid-forming enzyme NukM also recognized other peptides as a substrate.
|
