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Three-dimensional structure and functionof ABC transporter responsible for macromolecule transport in bacteria

Research Project

Project/Area Number 26292044
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionSetsunan University

Principal Investigator

Murata Kousaku  摂南大学, 理工学部, 教授 (90142299)

Co-Investigator(Kenkyū-buntansha) 橋本 渉  京都大学, (連合)農学研究科(研究院), 教授 (30273519)
丸山 如江  摂南大学, 理工学部, 助教 (90397563)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
KeywordsABCトランスポーター / X線結晶構造 / 基質結合タンパク質 / ABCタンパク質 / アルギン酸 / 体腔 / スフィンゴモナス属細菌 / ABCタンパク質 / 透過酵素 / X線結晶構造 / 酸性多糖輸送 / Sphingomonas / 輸送体分子進化 / Spingomonas / X線結晶構造解析 / フラジェリン / 鉄輸送体
Outline of Final Research Achievements

The ABC transporter in gram negative bacterium Sphingomonas sp. A1 functions, under the cooperation with substrate-binding protein ptesent in periplasm, in the transport of macromolecules such as alginate present in milieu into cytoplasm, Through the X ray analysis, we have succeeded in the determination of the complex of ABC transporter and substrate-binding protein, and almost completely solved the molecularmechanism of the transport and its biological significans in microorganisms.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (20 results)

All 2017 2016 2015 2014

All Journal Article (9 results) (of which Peer Reviewed: 8 results,  Acknowledgement Compliant: 3 results,  Open Access: 4 results) Presentation (11 results)

  • [Journal Article] ヘテロ多糖の輸送にかかわる細菌由来超分子の構造基盤2016

    • Author(s)
      橋本 渉, 丸山 如江, 伊藤 貴文, 髙瀬 隆一, 村田 幸作
    • Journal Title

      化学と生物

      Volume: 54 Pages: 885-891

    • NAID

      130006772653

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Structural determinants in bacterial 2-keto-3-deoxy-D-gluconate dehydrogenase KduD for dual-coenzyme specificity.2015

    • Author(s)
      Ryuichi Takase, Yukie Maruyama, Sayoko Oiki, Bunzo Mikami, Kousaku Murata, and Wataru Hashimoto.
    • Journal Title

      Proteins, In press (Prot-00467-2015)

      Volume: - Issue: 7 Pages: 934-947

    • DOI

      10.1002/prot.25042

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Formation of a single polar flagellum by lateral and polar bacterial flagellar gene sets in Sphingomonas sp. strain A12015

    • Author(s)
      Yukie Maruyama, Masahiro Kobayashi, Kousaku Murata, and Wataru Hashimoto
    • Journal Title

      Microbiology

      Volume: 161 Issue: 8 Pages: 1552-1560

    • DOI

      10.1099/mic.0.000119

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Structure of a bacterial ABC transporter involved in the import of an acidic polysaccharide alginate2015

    • Author(s)
      Yukie Maruyama, Takafumi Itoh, Ai Kaneko, Yu Nishitani, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata
    • Journal Title

      Structure

      Volume: 23 Issue: 9 Pages: 1643-1654

    • DOI

      10.1016/j.str.2015.06.021

    • NAID

      120005627618

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Crystal structure of Streptobacillus solute-binding protein involved in import of glycosaminoglycans.2015

    • Author(s)
      Sayoko Oiki, Kasumi Uenishi, Yukie Maruyama, Bunzo Mikami, Kousaku Murata, and Wataru Hashimoto.
    • Journal Title

      SPring-8 User Experiment Report,

      Volume: -

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Structure-based conversion of the coenzyme requirement of a short-chain dehydrogenase/reductase involved in bacterial alginate metabolism.2015

    • Author(s)
      Takase, R., Mikami, B., Kawai, S., Murata, K., Hashimoto, W.
    • Journal Title

      J. Biol. Chem.

      Volume: 289 Issue: 48 Pages: 33198-33214

    • DOI

      10.1074/jbc.m114.585661

    • NAID

      120005690199

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural insights into alginate binding by bacterial cell-surface.2015

    • Author(s)
      Kanate Temtrirath, Kousaku Murata, and Wataru Hasimoto
    • Journal Title

      Carbohydrate Research

      Volume: 404 Pages: 39-45

    • DOI

      10.1016/j.carres.2014.11.008

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Crystal structure of Pedobacter heparinus heparin lyase Hep III with the active site in a deep cleft.2014

    • Author(s)
      Hashimoto W., Maruyama Y., Nakamichi Y., Mikami B., Murata K.
    • Journal Title

      Biochemistry

      Volume: 53 Issue: 4 Pages: 777-786

    • DOI

      10.1021/bi4012463

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alginate-dependent gene expression mechanism in Sphingomonas sp. Strain A1.2014

    • Author(s)
      Chie Hayashi, Ryuichi Takase, Keiko Momma, Yukie Maruyama, Kousaku Murata, and Wataru Hashimoto:
    • Journal Title

      J.Bacteriol

      Volume: 196(14) Issue: 14 Pages: 2691-2700

    • DOI

      10.1128/jb.01666-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] 細菌による多糖輸送:アルギン酸取り込み細菌と分泌細菌の特異な細胞表層構造2017

    • Author(s)
      橋本渉, 村田幸作
    • Organizer
      2017年度日本農芸化学会大会
    • Place of Presentation
      京都
    • Related Report
      2016 Annual Research Report
  • [Presentation] 大気窒素を活用する次世代型発酵:窒素固定細菌による廃グリセロールの再資源化2017

    • Author(s)
      吉田暢広, 菅原良実, 南部優子, 村田幸作, 橋本渉
    • Organizer
      2017年度日本農芸化学会大会
    • Place of Presentation
      京都
    • Related Report
      2016 Annual Research Report
  • [Presentation] グリコサミノグリカンの輸送に関わる細菌由来基質結合タンパク質のドメインダイナミクス2017

    • Author(s)
      老木紗予子, 蒲池玲子, 三上文三, 村田幸作, 橋本渉
    • Organizer
      2017年度日本農芸化学会大会
    • Place of Presentation
      京都
    • Related Report
      2016 Annual Research Report
  • [Presentation] 酸性多糖の代謝に関わる還元酵素の補酵素要求性に関わる構造要因2016

    • Author(s)
      高瀬隆一、丸山如江、老木紗予子、三上文三、村田幸作、○橋本 渉
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌市
    • Year and Date
      2016-03-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 動物細胞外マトリックス(グリコサミノグリカン)を基質とする病原性細菌ABCトランスポーター2016

    • Author(s)
      ○ 老木紗予子、丸山如江、三上文三、村田幸作、橋本 渉
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌市
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細菌ABCトランスポーターのATP加水分解は、closed型の基質結合タンパク質との相互作用によって惹起される2016

    • Author(s)
      上西加純、金子あい、丸山如江、水野伸宏、馬場清貴、熊坂 崇、三上文三、村田幸作、橋本 渉
    • Organizer
      日本農芸化学会関西支部第493回講演会
    • Place of Presentation
      京都市
    • Year and Date
      2016-02-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] グリコサミノグリカン輸送に関わる連鎖桿菌由来基質結合タンパク質のX線結晶構造解析2015

    • Author(s)
      老木紗予子、丸山如江、三上文三、村田幸作、橋本 渉
    • Organizer
      日本農芸化学会中部・関西支部合同大会
    • Place of Presentation
      射身市
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] 大きな分子を取り込む細菌の輸送機構解明2015

    • Author(s)
      丸山如江、橋本 渉、村田幸作
    • Organizer
      日刊工業新聞 7月31日朝刊 21面
    • Place of Presentation
      京都市(京都大学)
    • Year and Date
      2015-07-31
    • Related Report
      2015 Annual Research Report
  • [Presentation] 基質結合タンパク質依存的ABCトランスポーターによる多糖輸送2015

    • Author(s)
      丸山如江、立石 彬、金子 あい1、橋本 渉1、村田幸作 (京大院・農1、摂南大・理工)
    • Organizer
      日本農芸化学会2015年度大会 (ポスター発表)
    • Place of Presentation
      岡山大学津島キャンパス
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 基質結合タンパク質依存的ABCトランスポーターによる多糖輸送2015

    • Author(s)
      丸山如江、立石 彬、金子 あい1、橋本 渉1、村田幸作 (京大院・農1、摂南大・理工)
    • Organizer
      日本農芸化学会2015年度大会 一般講演
    • Place of Presentation
      岡山大学津島キャンパス
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Sphingomonas属細菌A1株は高分子多糖アルギン酸に走化性を示す2015

    • Author(s)
      ○小林将大1、丸山如江1,2、村田幸作1,2、橋本 渉1 (1京大院・農、現2摂南大院・理工)
    • Organizer
      日本農芸化学会支部例会(第488回講演会)
    • Place of Presentation
      京都大学楽友会館
    • Year and Date
      2015-01-31
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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