Cold temperature adaptation of hyperthermophiles
| Project/Area Number |
26292045
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kwansei Gakuin University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
跡見 晴幸 京都大学, 工学(系)研究科(研究院), 教授 (90243047)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
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| Keywords | アーキア / 分子シャペロニン / ヘリカーゼ / 低温適応 / 好熱菌 / ポリアミン |
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| Outline of Final Research Achievements |
Hyperthermophile is considerd as the most primitive life. Study of the adaptive mechanisms of hyperthermophiles to cold environments may provide insight into evolutionary processes. In the project, cold inducible gens in Thermococcus kodakarensis were listed and evaluated their biological functions. Cold inducible molecular chaperonin CpkA and RNA helicase Tk-DeaD play an important role for cold adaptation. Especially, a mutant chaperonin that is functional at lower temperatures enables T.kodakarensis to grow under further colder environments. Polyamine contents in cytoplasm also seem to be correlated with temperature adaptation as cytoplasmic amount of branched chain polyamine (BCPA) is decreased and that of spermidine is increased at cold stressed environment. BCPA induce the A-form in the secondary structure of DNA, while linear-PA have only a minimum effect in vitro, suggesting that temperature dependent expression is correlated with polyamine balance in cytoplasm.
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Report
(4 results)
Research Products
(63 results)
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[Journal Article] Enhanced detection of RNA by MMLV reverse transcriptase coupled with thermostable DNA polymerase and DNA/RNA helicase.2017
Author(s)
Okano,H., Katano,Y., Baba,M., Fujiwara,A., Hidese,R., Fujiwara,S., Yanagihara,I., Hayashi,T., Kojima,K., Yasukawa,K.
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Journal Title
Enzyme Microb.Technol.
Volume: 96
Pages: 111-120
DOI
Related Report
Peer Reviewed
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[Journal Article] High sensitive one-step RT-PCR using MMLV reverse transcriptase, DNA polymerase with reverse transcriptase activity,and DNA/RNA helicase.2017
Author(s)
Okano,H., Baba,M., Yamasaki,T., Hidese,R., Fujiwara,S., Yanagihara,I., Ujiiye,T., Hayashi,T., Kojima,K., Takita,T., Yasukawa,K.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 印刷中
Issue: 1
Pages: 128-133
DOI
Related Report
Peer Reviewed
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[Journal Article] Naturally occurring branched-chain polyamines induce a crosslinked meshwork structure in a giant DNA.2016
Author(s)
Muramatsu,A., Shimizu,Y., Yoshikawa, Y., Fukuda,W., Umezawa,N., Horai,Y., Higuchi,T., Fujiwara,S., Imanaka,T.,Yoshikawa,K.
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Journal Title
J.Chem. Phys. J. Chem. Phys.
Volume: 145
Issue: 23
Pages: 235103-235103
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Enzyme characteristics of an euryarchaeota specific helicase from Thermococcus kodakarensis and its application for noise reduction in PCR.2016
Author(s)
Fujiwara,A., Kawato,K., Kato,S., Yasukawa,K., Hides,R., Fujiwara,S.
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Journal Title
Appl.Environ.Microbiol.
Volume: 82
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Enhanced detection of RNA by MMLV reversetranscriptase coupled with thermostable DNA polymerase and DNA/RNA helicase.2016
Author(s)
Hiroyuki Okano, Yuta Katano, Misato Baba, Ayako Fujiwara, Ryota Hidese, Shinsuke Fujiwara, Itaru Yanagihara, Takeshi Ujiiye, Tsukasa Hayashi, Kenji Kojima, Teisuke Takita, and Kiyoshi Yasukawa.
Organizer
RNase H 2016
Place of Presentation
京都大学楽友会館（京都府京都市）
Year and Date
2016-09-06
Related Report
Int'l Joint Research
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[Book] Protein synthesis and polyamines in thermophiles: Effect of polyamines on nucleic acid maintenance and gene expression. In T. Kusano and H.Suzuki (ed.), Polyamines, a universal molecular nexus for growth, survival and specialised metabolism2014
Author(s)
Fujiwara,S., Hidese, R., Inoue,T., Fukuda, W.
Total Pages
336
Publisher
Springer Co.Ltd., New York
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