• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular mechanism and application of structural elements that govern carbohydrase-catalyzed transfer reaction

Research Project

Project/Area Number 26292049
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Applied biochemistry
Research InstitutionHokkaido University

Principal Investigator

KIMURA, Atsuo  北海道大学, (連合)農学研究科(研究院), 教授 (90186312)

Co-Investigator(Kenkyū-buntansha) 奥山 正幸  北海道大学, (連合)農学研究科(研究院), 講師 (00344490)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords糖質酵素 / 糖転移反応 / 構造因子
Outline of Final Research Achievements

This project aims at identification and application of structural elements that regulate and improve the carbohydrase-catalyzed transfer activities by elucidating four promising phenomena, which we newly found. Results obtained are as follows: 1) we identify the structural elements to control the α-1,3-transfer reaction displayed by α-1,3-glucoside-transfer enzyme; 2) we reveal the reaction mechanism of the catalytic residue-mutated glycosidase that shows the high transfer yield; 3) a pore canal at the active site of glycosidase plays a role that supplies the catalytic water by study at the molecular level. 4) we analyze the polysaccharide-producing enzyme and identify its structural elements to contribute to the synthesis of oligosaccharides.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (34 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (13 results) (of which Peer Reviewed: 13 results,  Open Access: 9 results,  Acknowledgement Compliant: 2 results) Presentation (18 results) (of which Int'l Joint Research: 8 results,  Invited: 6 results) Book (1 results) Remarks (1 results)

  • [Int'l Joint Research] ソウル大学/全南大学(韓国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Heterologous production and characterization of catalytic subunit of glucosidase II in Schizosaccharomyces pombe2017

    • Author(s)
      Masayuki Okuyama, Masashi Miyamoto, Takayoshi Tagami, Ichiro Matsuo, Ma Min, Ryo Serizawa, Masanari Tanuma, Haruhide Mori, Atsuo Kimura
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 長鎖阻害剤の利用による植物α-グルコシダーゼの機能構造相関の解明2016

    • Author(s)
      田上貴祥, 山下恵太郎, 奥山正幸, 森 春英, 姚 閔, 木村淳夫
    • Journal Title

      応用糖質科学

      Volume: 6 Pages: 103-108

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] α-Glucosidases and α-1,4-glucan lyases: structures, functions, and physiological actions2016

    • Author(s)
      Masayuki Okuyama, Wataru Saburi, Haruhide Mori, Atsuo Kimura
    • Journal Title

      Cellular and Molecular Life Sciences

      Volume: 73 Issue: 14 Pages: 2727-2751

    • DOI

      10.1007/s00018-016-2247-5

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Two novel glycoside hydrolases responsible for the catabolism of cyclobis-(1→6)-α-nigerosyl2016

    • Author(s)
      Takayoshi Tagami, Eri Miyano, Juri Sadahiro, Masayuki Okuyama, Tomohito Iwasaki, Atsuo Kimura
    • Journal Title

      Journal of Biological Chemistry

      Volume: 291 Issue: 32 Pages: 16438-16447

    • DOI

      10.1074/jbc.m116.727305

    • NAID

      120006666518

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Kinetic properties and substrate inhibition of α-galactosidase from Aspergillus niger2016

    • Author(s)
      Julan Liao, Masayuki Okuyama, Keigo Ishihara, Yoshinori Yamori, Shigeo Iki, Takayoshi Tagami, Haruhide Mori, Seiya Chiaba, Atsuo Kimura
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 80 Issue: 9 Pages: 1747-1752

    • DOI

      10.1080/09168451.2015.1136884

    • NAID

      120005955471

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Efficient synthesis of α-galactosyl oligosaccharides using a mutant Bacteroides thetaiotaomicron retaining α-galactosidase (BtGH97b)2016

    • Author(s)
      Masayuki Okuyama, Kana Matsunaga, Ken-ichi Watanabe, Keitaro Yamashita, Takayoshi Tagami, Asako Kikuchi, Min Ma, Patcharapa Klahan, Haruhide Mori, Min Yao, Atsuo Kimura
    • Journal Title

      FEBS Journal

      Volume: 284 Issue: 5 Pages: 766-783

    • DOI

      10.1111/febs.14018

    • NAID

      120006401450

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Purification and characterization of a chloride ion-dependent α-glucosidase from the midgut gland of Japanese scallop (Patinopecten yessoensis)2016

    • Author(s)
      Masuda Y, Okuyama M, Iizuka T, Nakai H, Saburi W, Fukukawa T, Maneesan J, Tagami T, Naraoka T, Mori H & Kimura A
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 80 Issue: 3 Pages: 479-485

    • DOI

      10.1080/09168451.2015.1116926

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] α-Glucosidases and α-1,4-glucan lyases: structures, functions, and physiological actions.2016

    • Author(s)
      Masayuki Okuyama, Wataru Saburi, Haruhide Mori, Atsuo Kimura
    • Journal Title

      Cellular and Molecular Life Sciences

      Volume: 印刷中

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biochemical properties and substrate recognition mechanism of GH31 α-glucosidase from Bacillus sp. AHU 2001 with broad substrate specificity2015

    • Author(s)
      Wataru Saburi, Masayuki Okuyama, Yuya Kumagai, Atsuo Kimura, Haruhide Mori
    • Journal Title

      Biochimie

      Volume: 108 Pages: 140-148

    • DOI

      10.1016/j.biochi.2014.11.010

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural insights into catalytic reaction that is involved in the reorientation of Trp238 at the substrate-binding site in GH13 dextran glucosidase2015

    • Author(s)
      Kobayashi M, Saburi W, Nakatsuka D, Hondoh H, Kato K, Okuyama M, Mori H, Kimura A, and Yao M
    • Journal Title

      FEBS Letters

      Volume: 589 Issue: 4 Pages: 484-489

    • DOI

      10.1016/j.febslet.2015.01.005

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Structural elements responsible for the glucosidic linkage-selectivity of a glycoside hydrolase family 13 exo-glucosidase2015

    • Author(s)
      Wataru Saburi, Hiroaki Rachi-Otsuka, Hironori Hondoh, Masayuki Okuyama, Haruhide Mori, Atsuo Kimura
    • Journal Title

      FEBS letters

      Volume: 589 Issue: 7 Pages: 865-869

    • DOI

      10.1016/j.febslet.2015.02.023

    • NAID

      120005725141

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Different molecular complexity of linear-isomaltomegalosaccharides and β-cyclodextrin on enhancing solubility of azo dye ethyl red: Towards dye biodegradation2014

    • Author(s)
      Weeranuch Lang, Yuya Kumagai, Juri Sadahiro, Janjira Maneesan, Masayuki Okuyama, Haruhide Mori, Nobuo Sakairi, Atsuo Kimura
    • Journal Title

      Bioresource Technology

      Volume: 169 Pages: 518-524

    • DOI

      10.1016/j.biortech.2014.07.025

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identity of the two dextran dextrinases produced by Gluconobacter oxydans ATCC 11894 and its localization change depending on the cell growth2014

    • Author(s)
      Juri Sadahiro, Haruhide Mori, Wataru Saburi, Masayuki Okuyama, Atsuo Kimura
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 456 Issue: 1 Pages: 500-505

    • DOI

      10.1016/j.bbrc.2014.11.115

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] GH97α-galactosidase求核触媒変異酵素が触媒する糖転移反応の反応機構2016

    • Author(s)
      松永夏奈,奥山正幸,渡辺健一,田上貴祥,山下恵太郎,姚閔,森春英,木村淳夫
    • Organizer
      日本応用糖質科学会平成28年度大会
    • Place of Presentation
      福山大学宮地茂記念館(広島県・福山市)
    • Year and Date
      2016-09-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] Structure element to regulate transglucosidation specificity of Aspergillus niger α-glucosidase2016

    • Author(s)
      Min Ma, Masayuki Okuyama, Megumi Sato, Takayoshi Tagami, Haruhide Mori, Atsuo Kimura
    • Organizer
      The Sixth Symposium on the Alpha-Amylase Family
    • Place of Presentation
      Smolenice Castle (スモーレニス・スロバキア)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Novel single- and double-anchor-type isomaltomegalosaccharides: production and function2016

    • Author(s)
      Weeranuch Lang, Yuya Kumagai, Juri Sadahiro, Masayuki Okuyama, Haruhide Mori, Hiroshi Hara, Atsuo Kimura
    • Organizer
      The Sixth Symposium on the Alpha-Amylase Family
    • Place of Presentation
      Smolenice Castle (スモーレニス・スロバキア)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Purification and characterization of α-1,3-glucosidase from Aspergillus niger2016

    • Author(s)
      Min Ma, Takayoshi Tagami, Masayuki Okuyama, Atsuo Kimura
    • Organizer
      2016 International Carbohydrate Symposium
    • Place of Presentation
      Marriott New Orleans (ニューオーリンズ・米国)
    • Year and Date
      2016-07-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Reaction mechanism of glycosidases by studying 1,5-anhydro-D-fructose (AF) production2016

    • Author(s)
      Janjira Maneesan, Hideyuki Matsuura, Takayoshi Tagami, Haruhide Mori, Atsuo Kimura
    • Organizer
      2016 International Carbohydrate Symposium
    • Place of Presentation
      Marriott New Orleans (ニューオーリンズ・米国)
    • Year and Date
      2016-07-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Efficient production and characterization of α-1,3-glucosidase from Aspergillus niger2016

    • Author(s)
      Min Ma, Takayoshi Tagami, Masayuki Okuyama, Atsuo Kimura
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Gluconobacter oxydans由来の菌体外酵素dextran dextrinaseは自らの生成物デキストランによって細胞表層から遊離する2016

    • Author(s)
      貞廣 樹里, 森 春英, 佐分利 亘, 奥山 正幸, 木村 淳夫
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 直鎖のアンカー型イソマルトメガロ糖:生産・機能・応用2016

    • Author(s)
      木村 淳夫
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Novel single- or double-anchor-type isomaltomegalosaccharide, which enables to solubilize BCS-II compounds2016

    • Author(s)
      Atsuo Kimura
    • Organizer
      International Symposium: Current Issues in Food Chemistry and Biotechnology
    • Place of Presentation
      Seoul National University (平昌・韓国)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Improved production of megalosaccharides, namely linear isomaltomegalosaccharides, and their function2015

    • Author(s)
      Atsuo Kimura
    • Organizer
      30th Anniversary Fall Meeting and International Symposium of The Korean Society for Biotechnology and Bioengineering
    • Place of Presentation
      Incheon (Korea)
    • Year and Date
      2015-10-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular mechanism of glycosylase and application: production and function of megalosaccharide2015

    • Author(s)
      Atsuo Kimura
    • Organizer
      Joint HU-ITB Symposium
    • Place of Presentation
      Bandung (Indonesia)
    • Year and Date
      2015-10-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Podospora anserina α-glucosidase の高い糖転移能に関わる構造因子2015

    • Author(s)
      福本健太, Song Kyung-Mo, 奥山正幸, 木村淳夫
    • Organizer
      日本応用糖質科学会平成27年度大会(第64回)
    • Place of Presentation
      奈良春日野国際フォーラム(奈良県・奈良市)
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] Isomaltooligosaccharide 6-α-glucosyltransferaseの構造と触媒部位の機能性アミノ酸残基の同定2015

    • Author(s)
      川田恭平,藤本瑞,鐘ケ江倫世,西村崇志,奥山正幸,森春英,木村淳夫
    • Organizer
      日本応用糖質科学会平成27年度大会(第64回)
    • Place of Presentation
      奈良春日野国際フォーラム(奈良県・奈良市)
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] Production and function of megalosaccharides2015

    • Author(s)
      Atsuo Kimura, Kazumi Funane, Zui Fujimoto, Satoshi Ishizuka, and Shinichi Kitamura
    • Organizer
      Satellite Workshop in DTU
    • Place of Presentation
      Lyngby (Denmark)
    • Year and Date
      2015-05-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular mechanism of novel megalosaccharide-producing enzyme and its application2014

    • Author(s)
      Yuya Kumagai, Juri Sadahiro, Weeranuch Lang, Masayuki Okuyama, Haruhide Mori, Satoshi Ishizuka, Hiroshi Hara, Young-Min Kim, Doman Kim, Atsuo Kimura
    • Organizer
      The 13th China-Japan-Korea Joint Symposium on Enzyme Engineering
    • Place of Presentation
      Jeju (Korea)
    • Year and Date
      2014-11-16 – 2014-11-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dextran dextrinase の Phe602 の生成物特異性に関する影響2014

    • Author(s)
      坂谷敦, 熊谷祐也, 貞廣樹里, Lang Weeranuch, 奥山正幸, 森春英, 木村淳夫
    • Organizer
      日本応用糖質科学会平成26 年度大会(第63 回)
    • Place of Presentation
      朱鷺メッセ 新潟コンベンションセンター(新潟県・新潟市)
    • Year and Date
      2014-09-24 – 2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Amphiphilic function of linear-isomaltomegalosaccharides (L-IMS) on ethyl red (ER) solubility2014

    • Author(s)
      Weeranuch Lang, Yuya Kumagai, Juri Sadahiro, Masayuki Okuyama, Haruhide Mori, Nobuo Sakairi, Atsuo Kimura
    • Organizer
      日本応用糖質科学会平成26 年度大会(第63 回)
    • Place of Presentation
      朱鷺メッセ 新潟コンベンションセンター(新潟県・新潟市)
    • Year and Date
      2014-09-24 – 2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Molecular anatomy of novel megalosaccharide-producing enzymes2014

    • Author(s)
      Yuya Kumagai, Juri Sadahiro, Weeranuch Lang, Young-Min Kim, Michiyo Kanegae, Masayuki Okuyama, Haruhide Mori, Kazumi Funane, Zui Fujimoto, Satoshi Ishizuka, Hiroshi Hara, Doman Kim, Atsuo Kimura
    • Organizer
      2014 International Symposium on Biodiversity Conservation for Food Security and Rural Development
    • Place of Presentation
      Kunming (China)
    • Year and Date
      2014-07-13 – 2014-07-19
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Book] Application of Microbiology and Biochemistry. in "Frontiers of Agricultural Science"2015

    • Author(s)
      Atsuo Kimura, Masayuki Okuyama
    • Total Pages
      277
    • Publisher
      Shoukadoh Book Sellers
    • Related Report
      2014 Annual Research Report
  • [Remarks] 分子酵素学研究室

    • URL

      http://www.agr.hokudai.ac.jp/rfoa/abs/abs2-3.html

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report 2014 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi