Mechanism analysis of redox imbalance caused by aberrant proteostasis and its recovery using fluorescence protein sensors visualizing redox states.
| Project/Area Number |
26292052
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
Sakai Yasuyoshi 京都大学, (連合)農学研究科(研究院), 教授 (60202082)
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| Co-Investigator(Kenkyū-buntansha) |
寳関 淳 京都大学, 農学研究科, 特任准教授 (40423058)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | レドックス / 蛍光タンパク質 / タンパク質代謝異常 / ケミカルバイオロジー |
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| Outline of Final Research Achievements |
Based on the redox sensors, roGFP and our developed Redoxfluor, we have created organelle-targeting super redox sensors and techniques monitoring intracellular local redox states in mammalian cells. Using these techniques, we have elucidated molecular mechanisms of abnormal redox states caused by aberrant intracellular protein metabolisms. Furthermore, we demonstrated food-derived compounds that recovered abnormal redox states as redox modulators.
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Report
(4 results)
Research Products
(29 results)
