Cellular and molecular bases for mediating health-promoting action of prebiotics and probiotics
| Project/Area Number |
26292062
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Hokkaido University |
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Principal Investigator |
Sonoyama Kei 北海道大学, (連合)農学研究科(研究院), 准教授 (90241364)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Keywords | プレバイオティクス / プロバイオティクス / エキソソーム / microRNA / オルガノイド / 炎症 / 腸上皮細胞 / 脂肪細胞 / miRNA / 食品 / 細胞・組織 / 細菌 / 生理学 |
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| Outline of Final Research Achievements |
Our previous animal studies showed that indigestible oligosaccharides and Lactobacillus plantarum No.14 reduce allergic symptoms and obesity, respectively, whereas the cellular and molecular mechanisms remain unknown. We hypothesized that circulating exosomes are involved in the actions. The present study showed that anti-obese effect of L. plantarum No.14 is, at least in part, mediated by circulating exosomes. In addition, we found that supplementation of indigestible oligosaccharides and Lactobacillus strains alter the circulating microRNA profiles. Furthermore, we showed that intestinal organoids are useful for investigating the microRNA expression and exosome release in the intestinal epithelial cells.
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Report
(4 results)
Research Products
(50 results)
