| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
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| Outline of Final Research Achievements |
We first examined the anti-inflammatory effects of a quercetin glucuronide (Q3GA) and a sulfate (Q3'S) in macrophages. Next, to identify the target molecules of quercetin, a major polyphenol in human diet, and of its glucuronide metabolite (Q3GA), the derivatives of quercetin and Q3GA were chemically synthesized. We also developed a reaction condition for the formation of cross-link between proteins and these quercetin derivatives. On the other hand, we developed an analytical condition of LC-MS/MS for detection of quercetin metabolites in human plasma. High-sensitive LC-MS/MS analysis enables us to investigate the effects of different methods for quercetin intake on the metabolic profiles of quercetin in humans.
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