The high-level usage of metabolites of dietary compounds for elucidating the molecular mechanism of functional foods
| Project/Area Number |
26292072
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Toyama Prefectural University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
室田 佳恵子 近畿大学, 理工学部, 准教授 (40294681)
榊 利之 富山県立大学, 工学部, 教授 (70293909)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
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| Keywords | ポリフェノール / 抱合代謝物 / 出芽酵母 / UDP-グルクロン-酸転移酵素 / 硫酸基転移酵素 / メチル基転移酵素 / Caco2細胞 / 共培養系 / 酵母 / ＵＤＰ-グルクロン酸転移酵素 / 代謝物調製 / UDP-グルクロン酸転移酵素 / 硫酸転移酵素 |
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| Outline of Final Research Achievements |
In order to analyze the pharmacokinetics of dietary compounds including the metabolites in the body, we have developed xenobiotic metabolizing enzymes expression systems in budding yeast. Phase II metabolites of polyphenols having variety of conjugates were synthesized by using genetically engineered budding yeast containing UDP-glucose dehydrogenase-UDP-glucuronosyltransferase, sulfotransferase or catechol methyltransferase isoforms. Pharmacokinetics of polyphenols and metabolites including absorption and metabolism into the body were examined in vitro and in vivo using standard compounds of phase II metabolites. The high-level usage of metabolites of dietary compounds would be a powerful tool for elucidating the molecular mechanism of functional foods
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Production of an active form of vitamin D2 by genetically engineered CYP105A1.2017
Author(s)
K. Yasuda K, Y. Yogo , H.Sugimoto, H. Mano, T. Takita, M. Ohta, M. Kamakura, S. Ikushiro, K. Yasukawa, Y. Shiro, T. Sakaki.
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Journal Title
Biochem Biophys Res Commun
Volume: 486
Issue: 2
Pages: 336-341
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Whole cell-dependent production of equol metabolites using genetically engineered budding yeast.2015
Author(s)
Ikushiro S, Kataoka M, Nishikawa M, Yasuda M, Hamada M, Nakajima N, Murota K, Uehara M, Kamakura M, Toshiyuki S
Organizer
ICoFF2015
Place of Presentation
Souel, Korea
Year and Date
2015-11-22
Related Report
Int'l Joint Research / Invited
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