| Budget Amount *help |
¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
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| Outline of Final Research Achievements |
In order to analyze the pharmacokinetics of dietary compounds including the metabolites in the body, we have developed xenobiotic metabolizing enzymes expression systems in budding yeast. Phase II metabolites of polyphenols having variety of conjugates were synthesized by using genetically engineered budding yeast containing UDP-glucose dehydrogenase-UDP-glucuronosyltransferase, sulfotransferase or catechol methyltransferase isoforms. Pharmacokinetics of polyphenols and metabolites including absorption and metabolism into the body were examined in vitro and in vivo using standard compounds of phase II metabolites. The high-level usage of metabolites of dietary compounds would be a powerful tool for elucidating the molecular mechanism of functional foods
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