Effect of high nitrogen loading on growth of cool temperate trees raised under free-air O3 exposure

• Project/Area Number: 26292075
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Forest science
• Research Institution: Hokkaido University
• Principal Investigator: Koike Takayoshi 北海道大学, (連合)農学研究科(研究院), 教授 (10270919)
• Co-Investigator(Kenkyū-buntansha): 佐藤 冬樹 北海道大学, 北方生物圏フィールド科学センター, 教授 (20187230) ; 高木 健太郎 北海道大学, 北方生物圏フィールド科学センター, 准教授 (20322844) ; 渡辺 誠 東京農工大学, (連合)農学研究科(研究院), 准教授 (50612256) ; 渡部 敏裕 北海道大学, (連合)農学研究科(研究院), 准教授 (60360939)
• Co-Investigator(Renkei-kenkyūsha): KITA Kazuhito 北海道総合研究機構, 林業試験場, 主査 (90414250)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000); Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 越境大気汚染 / 対流圏オゾン / 窒素沈着 / 冷温帯樹種 / 光合成生産 / 成長 / 開放系施設 / 老化 / オゾン / カラマツ類 / 光合成機能 / 養分分析 / プラズマ発光分析 / カラマツ属 / 共生菌 / グイマツ雑種Ｆ１ / ニホンカラマツ / 外生菌根菌 / 硫酸アンモニウム / 開放系オゾン付加 / 火山灰土壌 / 蛇紋岩土壌 / 褐色森林土 / 感受性 / 耐性 / グイマツ雑種F1 / 老化促進

Outline of Final Research Achievements

With rapid increase of ground-surface ozone (O3) and nitrogen loading (N) in the northeastern part of Asia, some tree species showed declining symptoms, especially around volcano, such as somma of Lake Mashu. Trees are suffering from combination stresses of the two. We examined effects of elevated O3 and N on hybrid larch and related species grown in brown forest soil (BF) and immature volcanic ash soil (VA) with use of a free-air O3 fumigation system of our university. With recent analysis of transboundary particle matter, we should examine (NH4)2SO4 as N source. O3 alone clearly suppressed growth of the trees tested but we did not find any marked decrease of soil pH of around 5.0 with 50kgN/hr・yr. No combination effects were also found. Based on our experiments, we may conclude that N loading accelerates tree growth as acting as N resources to plants to some extent. But we should carefully check an increase of SO4 ion affecting soil pH in future.

Research Products

• [Int'l Joint Research] アテネ農業大学(ギリシャ)
• [Int'l Joint Research] 植物保護研究所(イタリア)
• [Int'l Joint Research]
• [Int'l Joint Research] フィレンツェ持続的植物保護研究所(イタリア)
• [Int'l Joint Research] アテネ農業大学(ギリシャ)
• [Journal Article] Stem and crown growth of Japanese larch and its hybrid F1 grown in two soils and exposed to two free-air O3 regimes (2017)
  • Author(s): Agathokleous, E., Vanderstock, A., Kita, K., and Koike, T.
  • Journal Title: Environmental Science and Pollution Research Volume: 24 Issue: 7 Pages: 6634-6662
  • DOI: 10.1007/s11356-017-8401-2
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Foliar chemical composition of two oak species grown in a free-air enrichment system with elevated O₃ and CO₂ (2016)
  • Author(s): Shi, C., Kitao, M., Agathokleous, E., Watanabe, M., Tobita, H., Yazaki, K., Kitaoka, S. and Koike, T.
  • Journal Title: Journal of Agricultural Meteorology Volume: 72 Issue: 1 Pages: 50-58
  • DOI: 10.2480/agrmet.D-14-00018
  • NAID: 130005132481
  • ISSN: 0021-8588, 1881-0136
  • Peer Reviewed / Open Access
• [Journal Article] A review study on past 40 years of research on effects of tropospheric O3 on belowground structure, functioning and processes of trees: a linkage with potential ecological implications (2016)
  • Author(s): Agathokleous, E., Saitanis, CJ., Wang, X., Watanabe, M. and Koike, T.
  • Journal Title: Water, Air, & Soil Pollution Volume: 227 Issue: 1 Pages: 1-28
  • DOI: 10.1007/s11270-015-2715-9
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Impacts of ethylene diurea (EDU) soil drench and foliar spray in Salix sachalinensis protection against O3-induced injury (2016)
  • Author(s): Agathokleous, E., Paoletti, E., Saitanis, C.J., Manning, W.J., Sugai, T.and Koike, T.
  • Journal Title: Science of the Total Environment Volume: 573 Pages: 1053-1062
  • DOI: 10.1016/j.scitotenv.2016.08.183
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ethylene-di-urea (EDU), the most effective phytoprotectant against O3 deleterious effects and a valuable research tool: a mystery of decades (2015)
  • Author(s): (22)Agathokleous, E., Koike, T., Watanabe, M., Hoshika, Y., and Saitanis, C.J.
  • Journal Title: Journal of Agricultural Meteorology Volume: 71 Pages: 185-195
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ozone-induced stomatal sluggishness changes carbon and water balance of temperate deciduous forests (2015)
  • Author(s): Hoshika, Y., Katata, G., Deushi, M., Watanabe, M., Koike, T. and Paoletti, E.
  • Journal Title: Scientific Reports Volume: 5(9871) Issue: 1 Pages: 1-8
  • DOI: 10.1038/srep09871
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Difference in photosynthetic responses to free air ozone fumigation between upper and lower canopy leaves of Japanese oak (Quercus mongolica var. crispula) saplings (2015)
  • Author(s): Watanabe, M., Hoshika, Y., Inada, N. and Koike, T.
  • Journal Title: Journal of Agricultural Meteorology Volume: 71 Pages: 227-231
  • NAID: 120005670602
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The effect of ozone-induced stomatal closure on ozone uptake and its changes due to leaf age in sun and shade leaves of Siebold’s beech (2015)
  • Author(s): Hoshika, Y., Watanabe, M., Inada, N. and Koike, T.
  • Journal Title: Journal of Agricultural Meteorology Volume: 71 Pages: 218-226
  • NAID: 120005670595
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Growth of Japanese and hybrid larch seedlings grown under free-air O3 fumigation―an initial assessment of the effects of adequate and excessive nitrogen. (2015)
  • Author(s): Kam D-G, Shi, C., Watanabe, M., Kita, K., Satoh, F. and Koike, T.
  • Journal Title: Journal of Agricultural Meteorology Volume: 71 Pages: 239-244
  • NAID: 120005670598
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Ethylenediurea (EDU) as a protectant of plants against O3. (2015)
  • Author(s): Agathokleous, E., Koike, T., Saitanis, C.J., Watanabe, M., Satoh, F. and Hoshika, Y.
  • Journal Title: Eurasian J. Forest Research Volume: 18 Pages: 37-50
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ozone induces stomatal narrowing in European and Siebold’s beeches: a comparison between two experiments of free-air ozone exposure (2015)
  • Author(s): Hoshika, Y., Watanabe, M., Kitao, M., Haberle, K-H., Grams, TEE., Koike, T. and Matyssek, R.
  • Journal Title: Environmental Pollution Volume: 196 Pages: 527-533
  • DOI: 10.1016/j.envpol.2014.07.034
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Ethylene-di-urea (EDU), the most effective phytoprotectant against O3 deleterious effects and a valuable research tool: a mystery of decades. (2015)
  • Author(s): (3)Agathokleous, E., Koike, T., Watanabe, M., Hoshika, Y., and Saitanis, C.J.
  • Journal Title: Journal of Agricultural Meteorology Volume: 71(2) Pages: 1-11
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Photosynthetic responses of Monarch birch seedlings to different timing of free air ozone fumigation (2014)
  • Author(s): Watanabe, M., Hoshika, Y. and Koike, T
  • Journal Title: Journal of Plant Research Volume: 127 Issue: 2 Pages: 339-345
  • DOI: 10.1007/s10265-013-0622-y
  • NAID: 120005661423
  • Peer Reviewed
• [Presentation] オゾン暴露に対するトドマツの生理成長応答～ニホンカラマツとの比較から． (2017)
  • Author(s): 菅井徹人・北尾光俊・来田和人・渡部敏裕・小池孝良
  • Organizer: 日本生態学会北海道地区大会, 2016年度
  • Place of Presentation: 北大・環境科学院、札幌
• [Presentation] Effects of ammonium sulfate and/ or ozone on growth and photosynthesis of Japanese larch and hybrid larch F1 (2016)
  • Author(s): Sugai T, Watanabe T, Kita T and Koike T.
  • Organizer: the 7th International Nitrogen Initiative Conference
  • Place of Presentation: Melbourne Cricket Ground
  • Year and Date: 2016-12-02
• [Presentation] Japanese larch and its hybrid grown in two soils in a free-air O3 enrichment regime: Growth and photosynthetic pigments. (2016)
  • Author(s): Agathokleous, E., Vanderstock, A., Sugai, T., Fujita, S., and Koike, T.
  • Organizer: IUFRO Regional Congress for Asia and Oceania 2016, Air pollution and climate change impacts on forest ecosystems
  • Place of Presentation: Beijing, People’s Republic of China
  • Year and Date: 2016-10-24
  • Int'l Joint Research / Invited
• [Presentation] Increased nitrogen and O3 changed ectomycorrhizae community structure in three larch species (2016)
  • Author(s): Koike T, Wang XN, Watanabe S, Qu LY and Tamai Y
  • Organizer: IUFRO Regional Congress for Asia and Oceania 2016,
  • Place of Presentation: Beijing, People’s Republic of China
  • Year and Date: 2016-10-24
  • Int'l Joint Research
• [Presentation] Effects of nitrogen deposition and phosphorus on fine root production of hybrid larch F1 seedlings (2016)
  • Author(s): Fujita S, Kita K, Nakaji T, Koike T
  • Organizer: IUFRO Regional Congress for Asia and Oceania 2016,
  • Place of Presentation: Beijing, People’s Republic of China
  • Year and Date: 2016-10-24
  • Int'l Joint Research
• [Presentation] Reduced insect grazing in white birch stands under free-air O3; enrichment (2016)
  • Author(s): Vanderstock, A, Fujita, S, Inoue, W, Agathakleous, E, Nakamura, M and Koike T
  • Organizer: IUFRO Regional Congress for Asia and Oceania 2016,
  • Place of Presentation: Beijing, People’s Republic of China
  • Year and Date: 2016-10-24
  • Int'l Joint Research
• [Presentation] Will the Change in the Balance of Nitrogen and Phosphorus Effect Fine Root Production of Hybrid Larch F1? (2016)
  • Author(s): Fujita S, Kita K, Koike T
  • Organizer: International Conference on Agricultural Biodiversity and Sustainability 2016
  • Place of Presentation: School of Agr. Hokkaido Univ, Sapporo
  • Year and Date: 2016-08-22
• [Presentation] Effects of different ozone concentrations on Japanese larch and its hybrid larch with an open top chamber (2016)
  • Author(s): Sugai T, Kam D, Kita K, Koike T,
  • Organizer: International Conference on Agricultural Biodiversity and Sustainability 2016
  • Place of Presentation: School of Agr. Hokkaido Univ, Sapporo
  • Year and Date: 2016-08-22
  • Int'l Joint Research
• [Presentation] Sustainable forest production with hybrid larch F1 under changing environment: Prediction with a free-air system and OTC (2016)
  • Author(s): Koike, T, Wang XN, Qu LY, Fujita S, Sugai T, Shi C and Watanabe T
  • Organizer: Joint symposium between Nanakai University and Hokkaido University
  • Place of Presentation: Tianjin, China
  • Year and Date: 2016-03-28
• [Presentation] グイマツ雑種F1の細根の成長に及ぼす窒素沈着の影響 :リン付加の有無の比較 (2016)
  • Author(s): 藤田早紀・来田和人・渋谷正人・斎藤秀之・小池孝良
  • Organizer: 第128回日本森林学会
  • Place of Presentation: 日本大学（神奈川県・藤沢市）
  • Year and Date: 2016-03-27
• [Presentation] Photosynthetic responses of Sakhalin fir seedlings exposed to elevated ozone: a preliminary assessment (2016)
  • Author(s): Sugai T, Kitao M, Watanabe T and Koike T.
  • Organizer: The 15th International Conference on Ecology and Silviculture of Fir
  • Place of Presentation: School of Agr. Hokkaido Univ, Sapporo
• [Presentation] Sustainable forestry production under changing environment: Prediction with a free-air system and OTC (2015)
  • Author(s): Koike T, E Agathokleous, C. Shi, Xiaona Wang, T. Watanabe
  • Organizer: 2nd Myanmar-Japan symposium
  • Place of Presentation: Pathein, Myanmar
  • Year and Date: 2015-12-02
  • Invited
• [Presentation] ニホンカラマツとグイマツ雑種F1の光合成と成長に及ぼすオゾン暴露と硫酸アンモニウム付加の影響 (2015)
  • Author(s): 菅井徹人，渡部敏裕，来田和人，斎藤秀之，渋谷正人，小池孝良
  • Organizer: 第64回北方森林学会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2015-11-12
• [Presentation] 異なるO3濃度及び土壌条件下で生育した落葉広葉樹稚樹の虫害 (2015)
  • Author(s): 井上航・中村誠宏・齋藤秀之・渋谷正人・小池孝良
  • Organizer: 北方森林学会65回大会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2015-11-12
• [Presentation] The effects of nitrogen deposition on the rhizoshpere of hybrid F1 -A Preliminary experiment- (2015)
  • Author(s): Fujita S, Kita K, Koike T
  • Organizer: The 1st Asia Pollution Workshop
  • Place of Presentation: 東京大学（東京都・文京区）
  • Year and Date: 2015-10-31
• [Presentation] Nutrient re-translocation of deciduous broad leaved trees grown under different soils in a free air O3 fumigation (2015)
  • Author(s): SHi, C., Watanabe, T., Satoh, F.and Koike, T.
  • Organizer: 126回日本森林学会
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Steady state and dynamic gas Exchange responses to Free-Air O3 Exposure in sensitive or tolerant trees (2014)
  • Author(s): Koike, T., Watanabe, M., Hoshika, Y., Satoh, F., and Izuta,T.
  • Organizer: The 9th Air Pollution and Global Change Symposium, "Plants and the Changing Environment"
  • Place of Presentation: Asilomar,CA,USA
  • Year and Date: 2014-06-08 – 2014-06-12
  • Invited
• [Book] Tree decline at the somma of Lake Mashu in northern Japan. Air pollution impacts on plant in East Asia (Izuta, T. ed.) (2017)
  • Author(s): Yamaguchi, T., Watanabe, M., Noguchi, I. and Koike, T.
  • Publisher: Springer-Verlang，Tokyo
• [Book] Combined effects of ozone and other environmental factors on Japanese trees. In: Air pollution impacts on plant in East Asia (Izuta, T. ed.) (2017)
  • Author(s): Watanabe, M., Hoshika, Y., Koike, T. and Izuta, T.
  • Publisher: Springer-Verlang，Tokyo
• [Book] Effects of ozone on Japanese trees. Air pollution impacts on plant in East Asia (Izuta, T. ed.) (2017)
  • Author(s): Watanabe, M., Hoshika, Y., Koike, T. and Izuta, T.
  • Publisher: Springer-Verlang，Tokyo
• [Book] 造林学 ４訂版 (2016)
  • Author(s): 丹下健・小池孝良
  • Total Pages: 180
  • Publisher: 朝倉書店
• [Remarks] Puplication AUA
  • URL: http://www.aua.gr/saitanis/publications.html
• [Remarks] Articles and book chapters since 2008
  • URL: http://www.ipsp.cnr.it/researchers/paoletti-elena/?lang=en
• [Remarks] 北大造林学研究室ＨＰ（小池孝良）
  • URL: http://www.agr.hokudai.ac.jp/fres/silv/index.php?%BE%AE%C3%D3%B9%A7%CE%C9