Strategic Design of Biointerfaces by Wood Carbohydrates for Cell Culture Applications
| Project/Area Number |
26292096
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Wood science
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
KITAOKA Takuya 九州大学, 大学院農学研究院, 教授 (90304766)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
ICHINOSE Hirofumi 九州大学, 大学院農学研究院, 准教授 (00432948)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
|
|---|
| Keywords | 糖鎖 / 自己組織化 / ナノ材料 / バイオインターフェース / 細胞培養 / 分化制御 / シグナル伝達 / バイオマテリアル / 細胞・組織 / 生体分子 / 生体材料 |
|---|
| Outline of Final Research Achievements |
Cell culture technology in vitro has recently attracted much attention in regenerative medicine. Herein, we propose the structural and biofunctional design of glyco-based cell culture scaffolds by using cello-/chito-oligosaccharides and/or crystalline polysaccharide nanofibers. In this project, we have completed the following achievements: (1) cell alignment and fusion of mouse myoblasts on self-assembled GlcNAc6-monolayers with various geometries, (2) promotion of the cell attachment of mouse fibroblasts NIH-3T3 on surface-carboxylated cellulose nanofibers, and (3) activation of the immune response of TLR2-expressing HEK293 cells on chitin nanofibers. Wood carbohydrates have potentials to act both as building blocks and bio-ligands for cell culture applications. Our challenge will open up a new phase of “Glyconanoarchitectonics”, based on the functional manipulation of cellular tissues by glyco-biointerface materials.
|
Report
(4 results)
Research Products
(38 results)
