| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
Cell culture technology in vitro has recently attracted much attention in regenerative medicine. Herein, we propose the structural and biofunctional design of glyco-based cell culture scaffolds by using cello-/chito-oligosaccharides and/or crystalline polysaccharide nanofibers. In this project, we have completed the following achievements: (1) cell alignment and fusion of mouse myoblasts on self-assembled GlcNAc6-monolayers with various geometries, (2) promotion of the cell attachment of mouse fibroblasts NIH-3T3 on surface-carboxylated cellulose nanofibers, and (3) activation of the immune response of TLR2-expressing HEK293 cells on chitin nanofibers. Wood carbohydrates have potentials to act both as building blocks and bio-ligands for cell culture applications. Our challenge will open up a new phase of “Glyconanoarchitectonics”, based on the functional manipulation of cellular tissues by glyco-biointerface materials.
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