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Effects of physical, chemical factors and viral infections on decreasing for marine planktonic diatom

Research Project

Project/Area Number 26292101
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Aquatic bioproduction science
Research InstitutionFisheries Research and Education Agency

Principal Investigator

Tomaru Yuji  国立研究開発法人水産研究・教育機構, 瀬戸内海区水産研究所, 主任研究員 (10416042)

Co-Investigator(Kenkyū-buntansha) 山口 晴生  高知大学, 自然科学系, 准教授 (10432816)
紫加田 知幸  国立研究開発法人水産研究・教育機構, その他部局等, 研究員 (40603048)
Co-Investigator(Renkei-kenkyūsha) KIMURA Kei  佐賀大学, 低平地沿岸海域研究センター, 講師 (30612676)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywords珪藻 / 死滅 / ウイルス / 光 / 栄養塩 / 沈降
Outline of Final Research Achievements

Diatom is one of significant primary producers in marine environments. In this study, we focused on the effects of physical, chemical and viral factors on the decreasing of Chaetoceros tenuissimus which is an ecologically important diatom species in Japanese coastal waters. Using culture based physiological studies under various environments including light, salinity, nutrients and viral infections, we examined the relationships between ecological factors and diatom cell decreasing/death. Furthermore, we tested the downward flux of the cells under nutrient deficiencies. The results showed that the most important factor on the cell decreases or death would be nitrogen deficiencies. Other factors tested in this study would be relatively moderate.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (28 results)

All 2017 2016 2015 2014 Other

All Journal Article (14 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 13 results,  Open Access: 5 results,  Acknowledgement Compliant: 4 results) Presentation (11 results) (of which Invited: 1 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Effects of temperature and salinity on diatom cell lysis by DNA and RNA viruses2017

    • Author(s)
      Kimura K, Tomaru Y
    • Journal Title

      Aquatic Microbial Ecology

      Volume: 79 Issue: 1 Pages: 79-83

    • DOI

      10.3354/ame01818

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Bothrosome Formation in Schizochytrium aggregatum (Labyrinthulomycetes, Stramenopiles) during Zoospore Settlement.2017

    • Author(s)
      Iwata, I., Kimura, K., Tomaru, Y., Motomura, T., Koike, K., Koike, K., & Honda, D.
    • Journal Title

      Protist

      Volume: 168 Issue: 2 Pages: 206-219

    • DOI

      10.1016/j.protis.2016.12.002

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphotriesterase activity in marine bacteria of the genera Phaeobacter, Ruegeria, and Thalassospira.2016

    • Author(s)
      Yamaguchi, H., Arisaka, H., Seki, M., Adachi, M., Kimura, K., & Tomaru, Y.
    • Journal Title

      International Biodeterioration & Biodegradation

      Volume: 115 Pages: 186-191

    • DOI

      10.1016/j.ibiod.2016.08.019

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 滅菌処理した表面微細凸凹寒天培地による有害赤潮藻類の培養2016

    • Author(s)
      山口晴生, 早川由真, 関美紀, 足立真佐雄, 木村圭, 外丸裕司
    • Journal Title

      藻類

      Volume: 64 Pages: 89-93

    • NAID

      40020897904

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Rapid quantification of viable cells of the planktonic diatom <i>Chaetoceros tenuissimus</i> and associated RNA viruses in culture2016

    • Author(s)
      Tomaru Y, Kimura K
    • Journal Title

      Plankton and Benthos Research

      Volume: 11 Issue: 1 Pages: 9-16

    • DOI

      10.3800/pbr.11.9

    • NAID

      130005128615

    • ISSN
      1880-8247, 1882-627X
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum2015

    • Author(s)
      T. Kadono, A. Miyagawa-Yamaguchi, N. Kira, Y. Tomaru, T. Okami, T. Yoshimatsu, L. Hou, T. Ohama, K. Fukunaga, M. Okauchi, H. Yamaguchi, K. Ohnishi, A. Falciatore, and M. Adachi
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 18708-18708

    • DOI

      10.1038/srep18708

    • NAID

      120005983236

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Ecological dynamics of two distinct viruses infecting marine eukaryotic decomposer Thraustochytrids (Labyrinthulomycetes, Stramenopiles).2015

    • Author(s)
      Takao Y, Tomaru Y, Nagasaki K, Honda D
    • Journal Title

      PLoS One

      Volume: 10 Issue: 7 Pages: e0133395-e0133395

    • DOI

      10.1371/journal.pone.0133395

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Marine Viruses that infect Eukaryotic Microalgae2015

    • Author(s)
      木村圭・外丸裕司
    • Journal Title

      Uirusu

      Volume: 65 Issue: 1 Pages: 37-46

    • DOI

      10.2222/jsv.65.37

    • NAID

      130005128442

    • ISSN
      0042-6857, 1884-3433
    • Related Report
      2015 Annual Research Report
  • [Journal Article] A New Fractionation and Recovery Method of Viral Genomes Based on Nucleic Acid Composition and Structure Using Tandem Column Chromatography2015

    • Author(s)
      Urayama S, Yoshida-Takashima Y, Yoshida M, Tomaru Y, Moriyama H, Takai K, Nunoura T
    • Journal Title

      Microbes and Environments

      Volume: 30 Issue: 2 Pages: 199-203

    • DOI

      10.1264/jsme2.ME14174

    • NAID

      130005077995

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Marine diatom viruses and their hosts: Resistance mechanisms and population dynamics2015

    • Author(s)
      Tomaru Y, Toyoda K, Kimura K
    • Journal Title

      Perspective in Phycology

      Volume: 2 Issue: 2 Pages: 69-81

    • DOI

      10.1127/pip/2015/0023

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 珪藻ウイルスの生態学 &#12316;ガラスの鎧を持つ生物に感染するウイルス&#12316;2015

    • Author(s)
      外丸裕司
    • Journal Title

      生物の科学~遺伝

      Volume: 69 Pages: 284-289

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Discovery of Two Novel Viruses Expands the Diversity of Single-Stranded DNA and Single-Stranded RNA Viruses Infecting a Cosmopolitan Marine Diatom.2015

    • Author(s)
      Kei Kimura, Yuji Tomaru
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 81 Issue: 3 Pages: 1120-1131

    • DOI

      10.1128/aem.02380-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Temperature alters algicidal activity of DNA and RNA viruses infecting Chaetoceros tenuissimus Meunier.2014

    • Author(s)
      Yuji Tomaru, Kei Kimura, Haruo Yamaguchi
    • Journal Title

      Aquatic Microbial Ecology

      Volume: 73 Issue: 2 Pages: 171-183

    • DOI

      10.3354/ame01713

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Coculture with marine bacteria confers resistance to complete viral lysis of diatom cultures.2014

    • Author(s)
      Kei Kimura, Yuji Tomaru
    • Journal Title

      Aquatic Microbial Ecology

      Volume: 73 Issue: 1 Pages: 69-80

    • DOI

      10.3354/ame01705

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] 海産珪藻Chaetoceros tenuissimusはウイルスとともに西日本沿岸域に広く分布する2017

    • Author(s)
      外丸裕司,木村圭,豊田健介
    • Organizer
      日本藻類学会第41回大会
    • Place of Presentation
      高知大学
    • Related Report
      2016 Annual Research Report
  • [Presentation] 本邦沿岸域における海産浮遊性珪藻Chaetoceros tenuissimusと本種感染性ウイルスの分布2017

    • Author(s)
      外丸裕司,木村圭,豊田健介
    • Organizer
      平成28年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Related Report
      2016 Annual Research Report
  • [Presentation] 水温・塩分が海産浮遊性珪藻Chaetoceros tenuissimusと本種感染性ウイルスの関係におよぼす影響2017

    • Author(s)
      外丸裕司,木村圭
    • Organizer
      平成28年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Related Report
      2016 Annual Research Report
  • [Presentation] 広島湾における長期高頻度調査に基づいた珪藻とウイルスの生態学的研究2016

    • Author(s)
      木村圭,外丸裕司
    • Organizer
      2016年日本ベントス学会・日本プランクトン学会合同大会
    • Place of Presentation
      熊本県立大学
    • Related Report
      2016 Annual Research Report
  • [Presentation] 長期調査による珪藻とそれに感染するウイルスの生態学的関係の解明2016

    • Author(s)
      木村圭,外丸裕司
    • Organizer
      日本微生物生態学会第31回大会
    • Place of Presentation
      横須賀市文化会館
    • Related Report
      2016 Annual Research Report
  • [Presentation] 珪藻個体群はウイルス存在下でも直ちに崩壊しない2015

    • Author(s)
      外丸裕司
    • Organizer
      日本海洋学会
    • Place of Presentation
      東京海洋大学品川キャンパス
    • Year and Date
      2015-03-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] 珪藻個体群のウイルス感受性は増殖フェーズによって変化するか?2015

    • Author(s)
      外丸裕司
    • Organizer
      日本藻類学会
    • Place of Presentation
      九州大学箱崎キャンパス
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] 珪藻に感染するウイルスの研究2014

    • Author(s)
      外丸裕司
    • Organizer
      第一回分子珪藻研究会
    • Place of Presentation
      関西学院大学梅田キャンパス
    • Year and Date
      2014-12-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] Viruses infecting harmful bloom forming diatoms in western Japan2014

    • Author(s)
      Yuji Tomaru
    • Organizer
      HAB2014
    • Place of Presentation
      ウェリントン(ニュージーランド)
    • Year and Date
      2014-10-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] ウイルスから見た珪藻の生態2014

    • Author(s)
      外丸裕司
    • Organizer
      日本微生物生態学会若手交流会
    • Place of Presentation
      アクトシティ浜松
    • Year and Date
      2014-10-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 珪藻Chaetoceros tenuissimusの対数増殖個体群がウイルス接種で直ちに崩壊しない現象に関する研究2014

    • Author(s)
      外丸裕司
    • Organizer
      日本水産学会
    • Place of Presentation
      九州大学箱崎キャンパス
    • Year and Date
      2014-09-21
    • Related Report
      2014 Annual Research Report
  • [Book] 感染症の生態学2016

    • Author(s)
      外丸裕司
    • Total Pages
      347
    • Publisher
      共立出版
    • Related Report
      2015 Annual Research Report
  • [Book] Marine Protist2015

    • Author(s)
      Tomaru Y
    • Total Pages
      637
    • Publisher
      Spriger
    • Related Report
      2015 Annual Research Report
  • [Remarks] 珪藻ウイルスの生態学的研究

    • URL

      http://feis.fra.affrc.go.jp/keisou_Virus/index.html

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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