Approach to molecular system controlling left-right asymmetry of flatfish body color using next generation techniques

• Project/Area Number: 26292110
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Tohoku University
• Principal Investigator: SUZUKI Tohru 東北大学, (連合)農学研究科(研究院), 教授 (70344330)
• Co-Investigator(Kenkyū-buntansha): 酒井 義文 東北大学, (連合)農学研究科(研究院), 准教授 (10277361) ; 横井 勇人 東北大学, (連合)農学研究科(研究院), 助教 (40569729)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000); Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2014: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
• Keywords: 異体類 / 左右非対称 / 発生 / 色素胞分化 / レチノイン酸 / RNA-Seq / 水産増養殖 / 変態 / 左右非対称性形成 / 発生・分化 / 水産学 / 遺伝子 / シグナル伝達 / バイオテクノロジー / カレイ類 / 色素前駆細胞 / 左右差形成 / 分子制御機構 / 概日リズム / 時計遺伝子

Outline of Final Research Achievements

Flounder larvae are externally symmetric commonly to general fishes, but they transform to left-right (L-R) asymmetric body by migrating one eye to the other side of body and then differentiating chromatophores at ocular side skin. In the hatcheries for aquaculture, since abnormal pigmentation, including pigmentation of ocular side and pseudo-albinism of blind side, often appears, elucidation of the mechanism to induce abnormal pigmentation is required. This research revealed cellular and molecular systems that control L-R asymmetric skin color development in flounder, and also abnormal pigmentation

Research Products

• [Journal Article] Excess retinoic acid induces fusion of centra by degenerating intervertebral ligament cells in Japanese flounder, Paralichthys olivaceus (2017)
  • Author(s): Wu X, Chen Q, Washio Y, Yokoi H, Suzuki T
  • Journal Title: Journal of Experimental Zoology, Part B, Molecular and Developmental Evolution Volume: 326B Issue: 8 Pages: 464-473
  • DOI: 10.1002/jez.b.22717
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Role of notochord cells and sclerotome derived cells in vertebral column development in fugu, Takifugu rubripes: histological and gene expression analyses (2016)
  • Author(s): T. Kaneko, K. Freeha, X. Wu, M. Mogi, S. Uji, H. Yokoi, T. Suzuki,
  • Journal Title: Source of the Document Cell and Tissue Research Volume: 366 Issue: 1 Pages: 37-49
  • DOI: 10.1007/s00441-016-2404-z
  • Peer Reviewed
• [Journal Article] Transcriptome analysis of vertebral bone in the flounder, Paralichthys olivaceus (Teleostei, Pleuronectiformes), using Illumina sequencing. (2015)
  • Author(s): Ibaraki H, Wu X, Uji S, Yokoi H, Sakai Y and Suzuki T
  • Journal Title: Mar Genomics Volume: 24 Pages: 269-276
  • DOI: 10.1016/j.margen.2015.09.009
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Early development of circadian rhythmicity in the suprachiamatic nuclei and pineal gland of teleost, flounder (Paralichthys olivaeus), embryos. (2015)
  • Author(s): Mogi M, Uji S, Yokoi H and Suzuki T
  • Journal Title: Dev Growth Differ Volume: 57 Issue: 6 Pages: 444-452
  • DOI: 10.1111/dgd.12222
  • Peer Reviewed
• [Journal Article] Establishment of oct4:gfp transgenic zebrafish line for monitoring cellular multipotency by GFP fluorescence. (2015)
  • Author(s): Kato H, Abe K, Yokota S, Matsuno R, Mikekado T, Yokoi H and Suzuki T.
  • Journal Title: In Vitro Cell Dev Biol Anim Volume: 51 Issue: 1 Pages: 42-49
  • DOI: 10.1007/s11626-014-9805-7
  • Peer Reviewed
• [Journal Article] Exposure to oxidative by-products during metamorphosis affects pigmentation patterns in flounder, Paralichthys olivaceus. (2015)
  • Author(s): Washio Y, Fujinami Y, Shimizu D, Yokoi H and Suzuki T.
  • Journal Title: Aquaculture Volume: 435 Pages: 318-327
  • DOI: 10.1016/j.aquaculture.2014.10.015
  • Peer Reviewed
• [Journal Article] Effect of temperature, hypoxia and disinfection with ozonated seawater during somitogenesis on muscular development of the trunk in larval seven-band grouper, Epinephelus septemfasciatus. (2014)
  • Author(s): 宇治督、鈴木徹、岩崎孝史、照屋和久、平沢敬一、白樫真、尾上静正、山下洋一、辻正治、土橋康史、奥沢公一
  • Journal Title: Aquaculture Research Volume: 印刷中 Issue: 11 Pages: 2698-2706
  • DOI: 10.1111/are.12425
  • Peer Reviewed
• [Journal Article] TALEN-mediated mutagenesis in zebrafish reveals a role for r-spondin 2 in fin ray and vertebral development (2014)
  • Author(s): Tatsumi, Y., Takeda, M., Matsuda, M., Suzuki, T., Yokoi, H.
  • Journal Title: FEBS Lett Volume: 558 Issue: 24 Pages: 4543-4550
  • DOI: 10.1016/j.febslet.2014.10.015
  • Peer Reviewed / Open Access
• [Journal Article] Visualization of musculature of the larval greater amberjack Seriola dumerili (Risso) using whole-mount immunostaining with special reference to cranial muscles (2014)
  • Author(s): Uji, S., Kurokawa, T., Hashimoto, H., Suzuki, T. and Okuzawa, K.
  • Journal Title: Aquaculture Reearch Volume: 45 Issue: 6 Pages: 1096-1108
  • DOI: 10.1111/are.12053
  • Peer Reviewed
• [Journal Article] Decrease in cytosine methylation at CpG island shores and increase in DNA fragmentation during zebrafish aging (2014)
  • Author(s): Shimoda, N., T. Izawa, A. Yoshizawa, H. Yokoi, Y. Kikuchi and N. Hashimoto
  • Journal Title: Age Volume: 36 Issue: 1 Pages: 103-115
  • DOI: 10.1007/s11357-013-9548-5
  • Peer Reviewed
• [Journal Article] No evidence for AID/MBD4-coupled DNA demethylation in zebrafish embryos. (2014)
  • Author(s): Shimoda, N., Hirose, K., Kaneto, R., Izawa, T., Yokoi, H., Hashimoto, N. and Kikuchi, Y
  • Journal Title: PLoS ONE Volume: 9 Issue: 12 Pages: e114816-e114816
  • DOI: 10.1371/journal.pone.0114816
  • Peer Reviewed / Open Access
• [Presentation] ヒラメ再生皮膚に現れる色素前駆細胞と色素胞の分布 (2017)
  • Author(s): 十川麻衣・茂木淳・横井勇人・鈴木徹
  • Organizer: 平成29年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都港区）
  • Year and Date: 2017-03-27
• [Presentation] 給餌条件により生じるヒラメ体色異常の遺伝子発現プロファイリング解析 (2017)
  • Author(s): 國政実里・横井勇人・酒井義文・青海忠久・鈴木徹
  • Organizer: 平成29年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都港区）
  • Year and Date: 2017-03-27
• [Presentation] ヒラメを使った、末梢組織の時計遺伝子発現におよぼすコルチゾルの影響の解析 (2017)
  • Author(s): 茂木淳・横井勇人・鈴木徹
  • Organizer: 平成29年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都港区）
  • Year and Date: 2017-03-27
• [Presentation] Expression of RA-receptors and RA-catabolic enzymes in Japanese flounder larvae (2017)
  • Author(s): Qiran Chen・横井勇人・鈴木徹
  • Organizer: 平成29年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都港区）
• [Presentation] Rhythmicity of clock genes, per2, per1 and cry1, in peripheral tissue in flounder (Paralichthy olivaceus), a marine teleost fish (2016)
  • Author(s): Makoto Mogi, Hayato Yokoi, Tohru Suzuki
  • Organizer: The 22nd International Congress of Zoology
  • Place of Presentation: Okinawa Convention Center（沖縄県那覇市）
  • Year and Date: 2016-10-14
• [Presentation] ヒラメの着色型黒化進行部位における色素前駆細胞の分布 (2016)
  • Author(s): 十川麻衣・茂木淳・横井勇人・鈴木徹
  • Organizer: 平成28年度日本水産学会秋季大会
  • Place of Presentation: 近畿大学（奈良県奈良市）
  • Year and Date: 2016-09-09
• [Presentation] ブラジル産とユタ産アルテミアを与えたヒラメ変態期仔魚で発現量の異なる遺伝子のRNA-Seqによるスクリーニング (2016)
  • Author(s): 國政実里・青海忠久・横井勇人・酒井義文・鈴木徹
  • Organizer: 平成28年度日本水産学会秋季大会
  • Place of Presentation: 近畿大学（奈良県奈良市）
  • Year and Date: 2016-09-09
• [Presentation] Expression of RA synthases, aldh1a1, 1a2 and 1a3, in hatched and metamorphic larvae and adult of Japanese flounder (2016)
  • Author(s): Qiran Chen・横井勇人・鈴木徹
  • Organizer: 平成28年度日本水産学会秋季大会
  • Place of Presentation: 近畿大学（奈良県奈良市）
  • Year and Date: 2016-09-09
• [Presentation] Transcriptome analysis of flounder metamorphosis (2016)
  • Author(s): Hayato Yokoi, Minori Kunimasa, Yoshifumi Sakai and Tohru Suzuki
  • Organizer: The 22nd Japanese medaka and zebrafish meeting
  • Place of Presentation: National Institute for Basic Biology（愛知県岡崎市）
  • Year and Date: 2016-08-20
• [Presentation] ヒラメにおける鱗の発生と色素胞、色素前駆細胞の挙動 (2016)
  • Author(s): 十川麻衣・茂木淳・横井勇人・鈴木徹
  • Organizer: 平成28年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都港区）
  • Year and Date: 2016-03-26
• [Presentation] ヒラメにおける時計遺伝子の日周リズムおよび光同調性の解析 (2016)
  • Author(s): 茂木淳・横井勇人・鈴木徹
  • Organizer: 平成28年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都港区）
  • Year and Date: 2016-03-26
• [Presentation] Vitamin A metabolism, revealed by RNA-seq, in the notochord constituting intervertebral tissue (2016)
  • Author(s): Khalid Freeha・横井勇人・鈴木徹
  • Organizer: 平成28年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都港区）
  • Year and Date: 2016-03-26
• [Presentation] 異体類における左右性異常を発生システムと遺伝子発現から追う (2016)
  • Author(s): 鈴木徹
  • Organizer: 平成28年度日本水産学会春季大会・シンポジウム「魚類人工種苗の形態異常・これまでとこれから」
  • Place of Presentation: 東京海洋大学（東京都港区）
  • Year and Date: 2016-03-26
  • Invited
• [Presentation] Embryonic Development of Circadian Rhythmicity in the Suprachiasmatic Nucleus and Pineal Gland in Flounder, a Teleost Fish (2016)
  • Author(s): Makoto Mogi, Hayato Yokoi, Tohru Suzuki
  • Organizer: International Symposium on Biological Rhythms
  • Place of Presentation: Nagoya University（愛知県名古屋市）
• [Presentation] ヒラメを用いた魚類概日リズムの光同調機構の解析 (2015)
  • Author(s): 茂木淳・宇治督・横井勇人・鈴木徹
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市））
  • Year and Date: 2015-12-01
• [Presentation] ヒラメ・カレイ類の左右非対称な体色形成と着色異常の発生機構 (2015)
  • Author(s): 鈴木 徹・鷲尾 洋平・烏 暁明・國政 実里・十川 麻衣・横井 勇人
  • Organizer: 日本動物学会第86回大会・第11回色素細胞シンポジウム；動物のからだを多様に彩るしくみ
  • Place of Presentation: 新潟コンベンションセンター（新潟県新潟市）
  • Year and Date: 2015-09-18
  • Invited
• [Presentation] Post-metamorphic ectopic pigmentation at the ocular side skin in flatfish (2015)
  • Author(s): Tohru Suzuki, Minori Kunimasa, Xiaoming Wu, Mai Togawa, Susumu Uji, Hayato Yokoi
  • Organizer: 48th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: つくば国際センター（茨城県つくば市）
  • Year and Date: 2015-06-02
• [Presentation] Effects of light conditions on the development of circadian rhythm at the suprachiasmatic nuclei and pineal in a marine fish, flounder (Paralichthys olivaceus) embryos (2015)
  • Author(s): Makoto Mogi, Susumu Uji, Hayato Yokoi, Tohru Suzuki
  • Organizer: 48th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: つくば国際センター（茨城県つくば市）
  • Year and Date: 2015-06-02
• [Presentation] レチノイン酸によりヒラメ仔魚に発現誘導される遺伝子の次世代シーケンサーを使ったスクリーニング (2015)
  • Author(s): 烏暁明・横井勇人・鈴木徹
  • Organizer: 平成27年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学、東京
  • Year and Date: 2015-03-27 – 2015-03-31
• [Presentation] ヒラメ体色形成におけるdhfr遺伝子の色素前駆細胞のマーカーとしての有用性の検討 (2015)
  • Author(s): 國政実里・烏暁明・横井勇人・鈴木徹
  • Organizer: 平成27年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学、東京
  • Year and Date: 2015-03-27 – 2015-03-31
• [Presentation] ヒラメ･カレイ類の変態後に起こる無眼側着色型黒化の発生過程と色素胞前駆細胞の分布 (2015)
  • Author(s): 鈴木徹・烏暁明・國政実里・横井勇人・宇治督・礒島俊実･田川正朋
  • Organizer: 平成27年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学、東京
  • Year and Date: 2015-03-27 – 2015-03-31
• [Presentation] TALEN、CRISPR/Cas9によるゲノム改編技術の利用 (2015)
  • Author(s): 鈴木徹
  • Organizer: 平成27年度日本水産学会水産増殖懇話会第1回講演会
  • Place of Presentation: 東京海洋大学、東京
  • Year and Date: 2015-03-27
  • Invited
• [Presentation] ヒラメ視交叉上核での概日リズムの発生 (2014)
  • Author(s): 茂木淳・宇治督・横井勇人・鈴木徹
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] oct4-gfp Tgメダカ系統の作製と胚発生、鰭再生過程におけるGFP発現の特性解析 (2014)
  • Author(s): 松野林太・横田晋平・加藤寛之・横井勇人・鈴木徹
  • Organizer: 平成26年度日本水産学会秋季大会
  • Place of Presentation: 九州大学、福岡
  • Year and Date: 2014-09-19 – 2014-09-22
• [Presentation] ヒラメ視交叉上核及び松果体での概日リズムの発生 (2014)
  • Author(s): 茂木淳・宇治督・横井勇人・鈴木徹
  • Organizer: 平成26年度日本水産学会秋季大会
  • Place of Presentation: 九州大学、福岡
  • Year and Date: 2014-09-19 – 2014-09-22
• [Presentation] ヒラメ視交叉上核での分子時計の初期発生 (2014)
  • Author(s): 茂木淳・宇治督・横井勇人・鈴木徹
  • Organizer: 日本動物学会 第85回大会
  • Place of Presentation: 東北大学、仙台
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 部分文字列の最長共通部分列長計算のためのデータ集合 (2014)
  • Author(s): 酒井義文
  • Organizer: 2014年度夏のLAシンポジウム
  • Place of Presentation: 半月庵、岩国
  • Year and Date: 2014-07-17 – 2014-07-19
• [Presentation] ゼブラフィッシュ胚でAIDとMBD4がカップルした脱メチル化は起こるのか？ (2014)
  • Author(s): 下田修義・廣瀬健太朗・井澤俊明・横井勇人・橋本有弘・菊池裕
  • Organizer: 第8回日本エピジェネティクス研究会
  • Place of Presentation: 伊藤国際学術研究センター、東京
  • Year and Date: 2014-05-25 – 2014-05-27
• [Book] Flatfishes: Biology and Exploitation, 2nd edition (2014)
  • Author(s): Suzuki, T. and Tanaka, M.
  • Publisher: Wiley Blackwell