| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
Novel rhamnose-binding lectins (RBLs), named DRLs, were isolated from the zebrafish eggs. Multiple DRLs were expressed in early embryos. DRLs were most highly expressed in ovary, but they were also distributed in various tissues in male and female fish. They had bacterial agglutination activity. These results suggest that DRLs are involved in innate immunity as a pattern recognition molecule against pathogens. An oxidative stress model was established by treating zebrafish embryos with various kinds of oxidative stress reagents. The oxidative stress was evaluated by measuring reactive oxygen species (ROS) generated in the embryos and intracellular Ca2+ concentration using a newly developed a simultaneous measuring instrument of fluorescence and chemiluminescence. Histidine-containing peptides and polyamines showed ROS scavenging activity in a concentration-dependent manner for any oxidation stress reagents. The morphological change due to UV irradiation was suppressed by spermine.
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