| Project/Area Number |
26292114
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic life science
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
Endo Hideaki 東京海洋大学, その他部局等, 教授 (50242326)
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| Co-Investigator(Renkei-kenkyūsha) |
OHNUKI Hitoshi 東京海洋大学, その他部局等, 教授 (60223898)
YOSHIURA Yasutoshi (国研)水産研究・教育機構, 瀬戸内海区水産研究所, 主任研究員 (90372052)
MURATA Masataka (公財)函館地域産業振興財団, 北海道立工業技術センター, 研究主任 (40505707)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Keywords | バイオセンサ / 魚類 / ストレス / グルコース / コルチゾル / コレステロール / 光通信 / バイオセンシング / 可視化 / モニタリング |
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| Outline of Final Research Achievements |
We developed a novel biosensing system to monitor fish stress using optical technology. Biosensors to monitor indicators of fish stress such as cortisol, glucose, and cholesterol concentrations were prepared. An immunosensor for determining the cortisol concentration was developed using an anti-cortisol antibody, carbon nanotubes, and an Au electrode. An enzyme sensor for simultaneous monitoring of glucose and cholesterol concentrations was also prepared using immobilized enzyme carbon nanotubes and micro-electrode. We then developed a novel wireless system using optical technology utilizing a light-emitting diode as the transmission device, which was attached to the body of the fish, and glucose levels of stress indicator were monitored in the fish under free-swimming conditions. Successful real-time monitoring of fish stress indicated that the new biosensing system worked well and is a promising tool for monitoring fish health.
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