Development of hydrocarbon production system from micro green alga Botryococcus braunii cultured in seawater medium

• Project/Area Number: 26292132
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Agricultural environmental engineering/Agricultural information engineering
• Research Institution: The University of Tokyo
• Principal Investigator: Imou Kenji 東京大学, 大学院農学生命科学研究科, 教授 (40184832)
• Co-Investigator(Kenkyū-buntansha): 海津 裕 東京大学, 農学生命科学研究科, 准教授 (70313070)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000); Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
• Keywords: 再生可能エネルギー / 微細藻類 / バイオ燃料

Outline of Final Research Achievements

Hydrocarbon production process from Botryococcus braunii (B.braunii) cultured in seawater medium was developed in this study. Seawater culture improved hydrocarbon extractability of B.braunii slurry by non-polar solvent, such as hexane, and increase the size of the colony. These effects could save the energy consumption in collection and concentration of B.braunii and hydrocarbon extraction from the concentrated slurry, and could improve total energy balance of hydrocarbon production from B.braunii.

Research Products

• [Journal Article] Effects of culture medium salinity on the hydrocarbon extractability, growth and morphology of Botryococcus braunii (2016)
  • Author(s): Furuhashi, K., Hasegawa, F., Saga, K., Kudou, S., Okada, S., Kaizu, Y., Imou, K.
  • Journal Title: Biomass and Bioenergy Volume: 91 Pages: 83-90
  • DOI: 10.1016/j.biombioe.2016.05.007
  • Peer Reviewed
• [Journal Article] The surface structure of Botryococcus braunii colony prevents the entry of extraction solvents into the colony interior (2016)
  • Author(s): Furuhashi K., Noguchi T., Okada S., Hasegawa F., Kaizu Y., Imou K.
  • Journal Title: Algal Research Volume: 16 Pages: 160-166
  • DOI: 10.1016/j.algal.2016.02.021
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effect of amphiphilic polysaccharides released from Botryococcus braunii Showa strain on hydrocarbon recovery (2015)
  • Author(s): Atobe, S., Saga, K., Hasegawa, F., Furuhashi, K., Tashiro, Y., Suzuki, Y., Okada, S., Imou, K.
  • Journal Title: Algal Research Volume: 10 Pages: 172-176
  • DOI: 10.1016/j.algal.2015.05.007
  • Peer Reviewed
• [Journal Article] The effect of the water-soluble polymer released from Botryococcus braunii Showa strain on solvent extraction of hydrocarbons (2015)
  • Author(s): Atobe, S., Saga, K., Hasegawa, F., Magota, A., Furuhashi, K., Okada, S., Suzuki, T., Imou, K.
  • Journal Title: Journal of Applied Phycology Volume: 27 Pages: 755-761
  • Peer Reviewed
• [Journal Article] Comparative Evaluation of Wet and Dry Processes for Recovering Hydrocarbon from Botryococcus Braunii (2015)
  • Author(s): Saga, K., Hasegawa, F., Miyagi, S., Atobe, S., Okada, S., Imou, K., Osaka, N., Yamagishi, T.
  • Journal Title: Applied Energy Volume: 141 Pages: 90-95
  • Peer Reviewed
• [Presentation] 培養温度の日変化がBotryococcus brauniiの増殖に与える影響 (2017)
  • Author(s): 山本悠策，長谷川文生，海津裕，芋生憲司，古橋賢一
  • Organizer: 第12回バイオマス科学会議
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2017-01-18
• [Presentation] アンモニア態窒素含有培地における Botryococcus brauniiの増殖阻害の回避 (2017)
  • Author(s): 三浦良治，長谷川文生，海津裕，芋生憲司，大坂典子
  • Organizer: 第12回バイオマス科学会議
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2017-01-18
• [Presentation] 温度の日変化がBotryococcus brauniiの増殖に与える影響 (2016)
  • Author(s): 山本悠策、古橋賢一、長谷川文生、海津裕、芋生憲司
  • Organizer: 農業食料工学会年次大会第75回年次大会
  • Place of Presentation: 京都大学（京都府京都市）
  • Year and Date: 2016-05-27
• [Presentation] Study on Oil Production by Hydrothermal Liquefaction of Solvent Extraction Residue from Microalgae (2016)
  • Author(s): Ryoji Miura, Kenichi Furuhashi, Fumio Hasegawa, Yutaka Kaizu, Kenji Imou
  • Organizer: ISMAB2016
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2016-05-23
  • Int'l Joint Research
• [Presentation] 微細藻Botryococuss brauniiを原料としたバイオリファイナリーの可能 (2016)
  • Author(s): 長谷川文生，古橋賢一，三浦良治，海津裕，芋生憲司，秋山直一，坂庭真悟，新井康永
  • Organizer: 第11回バイオマス科学会議
  • Place of Presentation: 東京
  • Year and Date: 2016-01-20
• [Presentation] 微細藻類の溶媒抽出残渣を用いた水熱液化によるオイル生産に関する研究 (2016)
  • Author(s): 三浦良治，古橋賢一，長谷川文生，海津裕，芋生憲司
  • Organizer: 第11回バイオマス科学会議
  • Place of Presentation: 東京
  • Year and Date: 2016-01-20
• [Presentation] 温度の日変化がBotryococcus brauniiの増殖に与える影響 (2016)
  • Author(s): 山本悠策、古橋賢一、長谷川文生、海津裕、芋生憲司
  • Organizer: 農業食料工学会関東支部 第52回年次大会
  • Place of Presentation: 筑波大学（茨城県つくば市）
• [Presentation] Prevention of Growth Inhibition ofBotryococcus braunii in Culture Containing Ammonium Ion (2016)
  • Author(s): Ryoji Miura, Fumio Hasegawa, Yutaka Kaizu, Kenji Imou
  • Organizer: 4th Asian Conference on Biomass Science
  • Place of Presentation: Penang, Malaysia
  • Int'l Joint Research
• [Presentation] 微細藻からのオイル生産プロセスの評価 (2015)
  • Author(s): 古橋賢一, 長谷川文生, 岡田茂, 海津裕, 芋生憲司
  • Organizer: 農業環境工学関連5 学会2015 年合同大会
  • Place of Presentation: 盛岡市
  • Year and Date: 2015-09-15
• [Presentation] 微細藻Botryococuss braunii を原料としたバイオリファイナリー (2015)
  • Author(s): 長谷川文生，古橋賢一，三浦良治，海津裕，芋生憲司
  • Organizer: 農業環境工学関連5 学会2015 年合同大会
  • Place of Presentation: 盛岡市
  • Year and Date: 2015-09-15
• [Presentation] 微細藻類の溶媒抽出残渣を用いた水熱液化によるオイル生産に関する研究 (2015)
  • Author(s): 三浦良治，古橋賢一，長谷川文生，海津裕，芋生憲司
  • Organizer: 農業環境工学関連5 学会2015 年合同大会
  • Place of Presentation: 盛岡市
  • Year and Date: 2015-09-15
• [Presentation] Changes of hydrocarbon extractability and extracellular polysaccharides covering Botryococcus braunii cultured in salinity medium (2015)
  • Author(s): K. Furuhashi, F. Hasegawa, K. Saga, S. Okada, Y. Kaizu, K. Imou
  • Organizer: 23rd European Biomass Conference & Exhibition
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2015-06-01
  • Int'l Joint Research
• [Presentation] 加熱前処理による微細藻類Botryococcus brauniiからの炭化水素回収メカニズム (2015)
  • Author(s): 跡部季子、長谷川文生、海津裕、岡田茂、芋生憲司
  • Organizer: バイオマス科学会議
  • Place of Presentation: つくば市
  • Year and Date: 2015-01-14 – 2015-01-15
• [Presentation] Study on the culture condition of enhancing hydrocarbon productivity of bio-algae (2015)
  • Author(s): 劉逸帆、古橋賢一、長谷川文生、海津裕、芋生憲司
  • Organizer: バイオマス科学会議
  • Place of Presentation: つくば市
  • Year and Date: 2015-01-14 – 2015-01-15
• [Presentation] Effect of salinity in culture media on matrix composition of Botryococcus braunii (2015)
  • Author(s): K Furuhashi, F Hasegawa, K Saga, S Okada, Y Kaizu, K Imou
  • Organizer: 2nd Asian Conference on Biomass Science
  • Place of Presentation: つくば市
  • Year and Date: 2015-01-13
• [Presentation] Hydrocarbon extraction from concentrated slurry of green microalga Botryococcus braunii cultured in diluted seawater (2014)
  • Author(s): K Furuhashi, K Saga, S Okada, F Hasegawa, Y Kaizu, K Imou
  • Organizer: Grand Renewable Energy 2014
  • Place of Presentation: 横浜市
  • Year and Date: 2014-07-27 – 2014-08-01
• [Presentation] 高効率炭化水素回収に向けた微細藻類の培養法 (2014)
  • Author(s): 古橋賢一, 工藤創, 佐賀清崇, 長谷川文生, 岡田茂, 海津裕, 芋生憲司
  • Organizer: 農業食料工学会年次大会
  • Place of Presentation: 琉球大学
  • Year and Date: 2014-05-16 – 2014-05-19