The establishment of cattle production using freeze-dried somatic cells and spematozoa
| Project/Area Number |
26292140
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Animal production science
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| Research Institution | Kochi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
市川 明彦 名城大学, 理工学部, 准教授 (20377823)
赤木 悟史 国立研究開発法人農業・食品産業技術総合研究機構, その他部局等, 研究員 (70414696)
及川 俊徳 宮城県畜産試験場, その他部局等, 研究員 (70588962)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Keywords | ウシ / 凍結乾燥保存 / 体細胞 / 精子 / フリーズドライ保存 / フリーズドライ / 核移植 / フリーズドライ細胞 / フリーズドライ精子 / 顕微授精 |
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| Outline of Final Research Achievements |
In general, mammalian somatic cells are preserved in the ultra low temperature freezer or the liquid nitrogen. However, the maintenance and transportation of frozen cells are costly, and there are problems with a safety aspect on liquid nitrogen. Freeze drying, which removes moisture through sublimation, has been used for a long-term storage of food, drug, yeast. To our knowledge, little work has been devoted to freeze dry for mammalian somatic cells. The objective of this study was to establish of cattle production using freeze-dried somatic cells and spematozoa, Our results suggest that bovine freeze-dried fibroblast cells would be useful as a donor cell for nuclear transfer, because they preserve their nuclear structure at -30℃. And the DNA damage in bovine spermatozoa are suppressed after freeze-drying, and freeze-dried spermatozoa produce blastocysts after ICSI. However, calves could not produceｄ using blastocysts derived from freeze-dried somatic cells and spermatozoa.
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Report
(4 results)
Research Products
(10 results)
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[Presentation] 凍結乾燥によるウシ体細胞の保存とその応用2016
Author(s)
松川 和嗣, 本郷 新, 田村 慎之介, 枝重 圭祐
Organizer
Cryopreservation Conference 2016
Place of Presentation
岡崎コンファレンスセンター （愛知県岡崎市)
Year and Date
2016-11-10
Related Report
Invited
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