Function of the fatty acid synthase inhibiting peptides derived from the enzyme-processed egg white and its applications

• Project/Area Number: 26292144
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Kagoshima University
• Principal Investigator: OHTSUKA Akira 鹿児島大学, 農水産獣医学域農学系, 教授 (10233173)
• Co-Investigator(Kenkyū-buntansha): 榎元 廣文 帝京大学, 理工学部, 講師 (30609392) ; 井尻 大地 鹿児島大学, 農水産獣医学域農学系, 准教授 (50551090)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2014: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 卵白 / 脂肪酸合成酵素 / ペプチド / 脂質代謝改善 / 畜産物利用 / 多価不飽和脂肪酸 / マウス / ニワトリ

Outline of Final Research Achievements

In this study, we aimed to verify the ameliorating effects of the pepsin and heat-processed ovalbumin fraction (PHOVA) and the fatty acid synthase (FAS) inhibiting peptide (WTSSN) contained in PHOVA on sugar and lipid metabolism. The feeding of PHOVA to the obese and type II diabetes model mice remarkably suppressed the blood glucose concentration, showing an ameliorating effect of carbohydrate metabolism. The feeding of PHOVA to chickens reduced the blood and hepatic triacylglycerol concentration, showing an ameliorating effect of lipid metabolism. WTSSN and polyunsaturated fatty acid (PUFA) suppressed triacylglycerol accumulation in HepG2 hepatocytes. Moreover, ω6 PUFAs showed the strongest inhibitory effect on FAS activity.

Research Products

• [Journal Article] Distribution Analysis of Anthocyanins, Sugars, and Organic Acids in Strawberry Fruits Using Matrix-Assisted Laser Desorption/Ionization- Imaging Mass Spectrometry (2018)
  • Author(s): Hirofumi Enomoto, Kei Sato, Koji Miyamoto, Akira Ohtsuka, Hisakazu Yamane
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 66 (19) Pages: 4958-4965
  • Peer Reviewed
• [Journal Article] Visualisation of abscisic acid and 12-oxo-phytodienoic acid in immature Phaseolus vulgaris L. seeds using desorption electrospray ionisation-imaging mass spectrometry (2017)
  • Author(s): Hirofumi Enomoto, Takuya Sensu, Kei Sato, Futoshi Sato, Thanai Paxton, Emi Yumoto, Koji Miyamoto, Masashi Asahina, Takao Yokota, Hisakazu Yamane
  • Journal Title: SCIENTIFIC REPORTS Volume: 7 Issue: 1 Pages: 1-8
  • DOI: 10.1038/srep42977
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] カプサイシンの飼料投与が初生ヒナの骨および骨格筋の発達に与える影響 (2015)
  • Author(s): 石丸善貴、古川愛理、島元紗希、井尻大地、大塚彰
  • Organizer: 日本家禽学会 2015年度春季大会
  • Place of Presentation: 宇都宮大学（栃木県、宇都宮市）
  • Year and Date: 2015-03-30
• [Presentation] 米糠外皮の給与がラットの脂肪蓄積と便通に及ぼす影響 (2015)
  • Author(s): 川口真奈、野島敏、井尻大地、山内陽子、藤田芳和、井尻晢、大塚彰
  • Organizer: 日本畜産学会 第119回大会
  • Place of Presentation: 宇都宮大学（栃木県、宇都宮市）
  • Year and Date: 2015-03-28
• [Presentation] 初生ヒナへのβ2アドレナリン受容体作動薬の持続的投与が骨格筋の発達へ及ぼす影響 (2015)
  • Author(s): 島元紗希、石丸善貴、井尻大地、大塚彰
  • Organizer: 日本畜産学会 第119回大会
  • Place of Presentation: 宇都宮大学（栃木県、宇都宮市）
  • Year and Date: 2015-03-28