Passive Immunity acquired via colostrum in pig

• Project/Area Number: 26292145
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Kyoto Prefectural University
• Principal Investigator: INOUE Ryo 京都府立大学, 生命環境科学研究科, 講師 (70443926)
• Co-Investigator(Kenkyū-buntansha): 塚原 隆充 京都府立大学, 生命環境科学研究科, 研究員 (90562091)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000); Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: ブタ / 初乳 / 移行免疫 / 免疫発達 / 細胞 / プロテオーム

Outline of Final Research Achievements

The major outcomes of the research are; 1. Estimation of proteins that transfers from colostrum to piglets, 2. determination of the dynamics of plasma concentration of two proteins selected from the estimation, 3. Confirmation of the transfer of colostral cells into piglet's small intestine. This research suggested that a considerable number of proteins could transfer from colostrum to piglets and colostral cells indeed transfer via small intestine of piglet.

Academic Significance and Societal Importance of the Research Achievements

本研究ではブタ初乳中に含まれるタンパク質の詳細な分析や、それらのうち仔豚に移行する成分の特定、さらには初乳から移行する細胞の分析を行った。本研究により「重要である」と漠然と認識されていたブタ初乳摂取がなぜ重要なのか、その科学的理由の一端が明らかになった。また、ブタ初乳において、何が科学的にわかっていて、何がわかっていないのかを整理し、より研究の焦点を当てるべき研究領域を明らかにすることができた。

Research Products

• [Journal Article] Generation of anti-porcine CD69 monoclonal antibodies and their usefulness to evaluate early activation of cellular immunity by flow cytometric analysis (2018)
  • Author(s): Hayashi Yumiko、Okutani Mie、Ogawa Shohei、Tsukahara Takamitsu、Inoue Ryo
  • Journal Title: Animal Science Journal Volume: 89 Issue: 5 Pages: 825-832
  • DOI: 10.1111/asj.12989
  • Peer Reviewed
• [Journal Article] Dietary Probiotic Compound Improves Reproductive Performance of Porcine Epidemic Diarrhea Virus-Infected Sows Reared in a Japanese Commercial Swine Farm under Vaccine Control Condition (2017)
  • Author(s): Inatomi Takio、Amatatsu Masaaki、Romero-Perez Gustavo A.、Inoue Ryo、Tsukahara Takamitsu
  • Journal Title: Frontiers in Immunology Volume: 8
  • DOI: 10.3389/fimmu.2017.01877
  • Peer Reviewed / Open Access
• [Journal Article] Development of the porcine intestine during early postnatal life -Evaluation focusing on histological, enzymatic and immunological parameters-. (2017)
  • Author(s): Ryo Inoue, Takamitsu Tsukahara
  • Journal Title: Proceedings of Japan Society of Animal Nutrition and Metabolism Volume: 60 Pages: 99-109
  • NAID: 40020901977
  • Peer Reviewed
• [Journal Article] The effect of colostrum ingestion during the first 24 h of life on early postnatal development of piglet immune systems. (2016)
  • Author(s): Ogawa, S. Tsukahara, T. Imaoka, T. Nakanishi, N. Ushida, K. and Inoue, R.
  • Journal Title: Animal Science Journal Volume: 15 Issue: 12 Pages: 1511-1515
  • DOI: 10.1111/asj.12573
  • Peer Reviewed
• [Journal Article] Comparison of gene expression profiles of T cells in porcine colostrum and peripheral blood. (2016)
  • Author(s): Ogawa, S. Okutani, M. Tsukahara, T. Nakanishi, N. Kato, Y. Fukuta, K. Romero-Pérez, GA. Ushida, K. and Inoue, R.
  • Journal Title: American Journal of Veterinary Resaerch Volume: -
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Weaning Markedly Affects Transcriptome Profiles and Peyer’s Patch Development in Piglet Ileum. (2015)
  • Author(s): Inoue, R. Tsukahara, T. Nakatani, M. Okutani, M. Nishibayashi, R. Ogawa, S. Harayama, T. Nagino, T. Hatanaka, H. Fukuta, K. Romero-Pérez, GA. Ushida, K. and Kelly, D.
  • Journal Title: Frontiers in Immunology Volume: 15
  • DOI: 10.3389/fimmu.2015.00630
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 豚繁殖・呼吸障害症候群ウイルス(PRRSV)野外単離株と市販生ワクチン株との全ゲノム配列の相同性 (2017)
  • Author(s): 小林良奈、塚原隆充、矢原芳博、井上亮
  • Organizer: 日本畜産学会第122回大会
• [Presentation] 仔豚免疫についての最新知見 (2017)
  • Author(s): 井上亮
  • Organizer: ゼノアック養豚セミナー
  • Invited
• [Presentation] Development of the porcine intestine during early postnatal life. Evaluation focusing on histological, enzymatic, and immunological parameters- (2017)
  • Author(s): Ryo Inoue, Takamitsu Tsukahara
  • Organizer: 17th AAAP 家畜栄養生理研究会
  • Int'l Joint Research / Invited
• [Presentation] 仔豚の免疫についての最新知見 (2015)
  • Author(s): 井上亮
  • Organizer: ゼノアック養豚セミナー
  • Place of Presentation: メインホテルナカムラ
  • Year and Date: 2015-11-26
  • Invited
• [Presentation] 仔豚の免疫についての最新知見 (2015)
  • Author(s): 井上亮
  • Organizer: ゼノアック養豚セミナー
  • Place of Presentation: ホテルマリアージュ仙水
  • Year and Date: 2015-11-25
  • Invited
• [Presentation] 初乳免疫の最新知見 (2015)
  • Author(s): 井上亮
  • Organizer: 日本養豚開業獣医師協会 第13回通常総会
  • Place of Presentation: アジュール竹芝
  • Year and Date: 2015-07-10
  • Invited
• [Presentation] 初乳の成分と重要性について (2014)
  • Author(s): 井上亮
  • Organizer: 宮崎しゃくなげ会
  • Place of Presentation: 宮崎観光ホテル
  • Year and Date: 2014-07-18
  • Invited
• [Remarks] ブタの母乳と仔豚の免疫系の発達に関する研究
  • URL: http://eureka.kpu.ac.jp/~r-inoue/id-6.html