Studies on the molecular mechanisms for the increase in MDV virulence in the field

• Project/Area Number: 26292147
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Hokkaido University
• Principal Investigator: Kazuhiko Ohashi 北海道大学, (連合)獣医学研究科, 教授 (90250498)
• Co-Investigator(Kenkyū-buntansha): 村田 史郎 北海道大学, (連合)獣医学研究科, 助教 (10579163) ; 今内 覚 北海道大学, (連合)獣医学研究科, 准教授 (40396304)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: マレック病 / マレック病ウイルス

Outline of Final Research Achievements

Marek’s disease virus (MDV), a causative agent of Marek’s disease (MD), which is characterized by the formation of malignant lymphomas, tends to increase its virulence in the fields. To clarify the molecular mechanism for the increase in MDV virulence, recent MDV strains isolated from vaccinated chickens which developed clinical MD were virologically and molecular biologically characterized. Several new diversities/mutations were detected in viral genes of these strains, and a plaque-purified MDV strain was shown to be pathogenic to chicks. However, phylogenetic analysis of the whole genome of the MDV strain and reference strains showed that domestic MDV may increase its virulence through unknown mechanisms which are different from those suggested previously in MDV strains isolated in the United States.

Research Products

• [Journal Article] Isolation and purification of <i>Gallid herpesvirus</i> 2 strains currently distributed in Japan (2017)
  • Author(s): Machida Y, Murata S, Matsuyama-Kato A, Isezaki M, Taneno A, Sakai E, Konnai S, Ohashi K.
  • Journal Title: Journal of Veterinary Medical Science Volume: 79 Issue: 1 Pages: 115-122
  • DOI: 10.1292/jvms.16-0329
  • NAID: 130005289788
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Bovine leukemia virus reduces anti-viral cytokine activities and NK cytotoxicity by inducing TGF-β secretion from regulatory T cells. (2015)
  • Author(s): Ohira K, Nakahara A, Konnai S, Okagawa T, Nishimori A, Maekawa N, Ikebuchi R, Murata S, Ohashi K.
  • Journal Title: Immun. Inflamm. Volume: 4 Issue: 1 Pages: 52-63
  • DOI: 10.1002/iid3.93
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Expression analysis of programmed death ligand 2 in tumors caused by the avian oncovirus Marek's disease virus (2014)
  • Author(s): Matsuyama-Kato, A., Murata, S., Isezaki, M., Takasaki, S., Kano, R., Konnai, S., & Ohashi, K
  • Journal Title: Arch. Virol Volume: (印刷中) Issue: 8 Pages: 2123-6
  • DOI: 10.1007/s00705-014-2021-7
  • Peer Reviewed
• [Journal Article] Expression of PD-L1 on Canine Tumor Cells and Enhancement of IFN-γ Production from Tumor-Infiltrating Cells by PD-L1 Blockade (2014)
  • Author(s): Maekawa N, Konnai S, Ikebuchi R, Okagawa T, Adachi M, Takagi S, Kagawa Y, Nakajima C, Suzuki Y, Murata S, Ohashi K
  • Journal Title: PLoS One Volume: 9 Issue: 6 Pages: e98415-e98415
  • DOI: 10.1371/journal.pone.0098415
  • Peer Reviewed / Open Access
• [Journal Article] Influence of PD-L1 cross-linking on cell death in PD-L1-expressing cell lines and bovine lymphocytes (2014)
  • Author(s): Ryoyo Ikebuchi, Satoru Konnai, Tomohiro Okagawa, Kazumasa Yokoyma, Chie Nakajima, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi.
  • Journal Title: Immunology Volume: (印刷中) Issue: 4 Pages: 551-61
  • DOI: 10.1111/imm.12243
  • Peer Reviewed
• [Presentation] マレック病ウイルス日本分離株の全ゲノム解析 (2016)
  • Author(s): 村田史郎、町田柚香、伊勢崎 政美、今内覚、大橋和彦
  • Organizer: 第１５９回日本獣医学会
  • Place of Presentation: 日本大学生物資源科学部、藤沢市、神奈川県
  • Year and Date: 2016-09-06
• [Presentation] Sequence analysis of a Marek’s disease virus strain isolated in Japan (2016)
  • Author(s): Murata, S., Machida, Y., Isezaki, M., Konnai, S., Ohashi, K.
  • Organizer: 11th International Symposium on Marek’s Disease and Avian Herpesviruses
  • Place of Presentation: Vinci Convention Center, Tours, France
  • Year and Date: 2016-07-06
  • Int'l Joint Research
• [Presentation] Analysis of the diversities observed in the Meq protein of Marek's disease virus (MDV) and immunosuppression during MDV infection. (2015)
  • Author(s): Ohashi, K., Murata, S., Matsuyama-Kato, S., Okada, T., Hashiguchi, M., Isezaki, M., Konnai, S.
  • Organizer: New Paradigm of Molecular Diagnostics and Therapeutics
  • Place of Presentation: Yonsei University, Wonju, Korea
  • Year and Date: 2015-11-05
  • Int'l Joint Research / Invited
• [Presentation] ワクモ(Dermanyssus gallinae)由来カテプシンL様タンパク質の性状解析とワクチン抗原としての評価 (2015)
  • Author(s): 北條 巧、谷口 綾香、村田 史郎、伊勢崎 政美、酒井 英史、矢吹 卓也、 寺田 晴菜、種子野 章、今内 覚、大橋 和彦
  • Organizer: 第158回日本獣医学会
  • Place of Presentation: 北里大学獣医学部, 十和田市，青森県
  • Year and Date: 2015-09-07
• [Presentation] マレック病ウイルスmeq遺伝子に認められた欠損配列 (2015)
  • Author(s): 村田 史郎、山本 英次、坂下 奈津美、松山 あゆ美、伊勢崎 政美、町田 柚香、 今内 覚、大橋 和彦
  • Organizer: 第158回日本獣医学会
  • Place of Presentation: 北里大学獣医学部, 十和田市，青森県
  • Year and Date: 2015-09-07
• [Presentation] 日本に分布するマレック病ウイルス野外株の分離 (2015)
  • Author(s): 町田 柚香、村田 史郎、伊勢崎 政美、酒井 英史、種子野 章、今内 覚、 大橋 和彦
  • Organizer: 第158回日本獣医学会
  • Place of Presentation: 北里大学獣医学部, 十和田市，青森県
  • Year and Date: 2015-09-07
• [Presentation] ウイルス性発癌: 鶏マレック病研究の最新知見 (2014)
  • Author(s): 大橋和彦
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2014-09-09 – 2014-09-12
  • Invited
• [Book] 感染症の生態学 (2016)
  • Author(s): 日本生態学会
  • Total Pages: 356
  • Publisher: 共立出版