Elucidation of the molecular mechanism for pathogenesis by avian rotavirus using a reverse genetics approach

• Project/Area Number: 26292148
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Gifu University
• Principal Investigator: SUGIYAMA MAKOTO 岐阜大学, 応用生物科学部, 教授 (80196774)
• Co-Investigator(Kenkyū-buntansha): 伊藤 直人 岐阜大学, 応用生物科学部, 准教授 (20334922)
• Research Collaborator: OKADARA Kota ; MITAKE Hiromichi ; OKADA Kazuma ; NAKAGAWA Kento ; TAMADA Daigo
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000); Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
• Keywords: 鳥ロタウイルスA / 遺伝的多様性 / 遺伝子操作系 / ロタウイルス / 病原性発現機構 / 逆遺伝子操作 / 獣医学 / 逆遺伝子操作系

Outline of Final Research Achievements

This research project was conducted to reveal molecular mechanisms by which avian rotavirus A (RAV) induces pathogenicity in mammals. Firstly, we analyzed genome sequences from 3 avian RAVs and compared them with sequences of avian RAVs from various species. We discovered unique genome sequences in the 2 RAVs, which were certificated as novel genotypes by the Rotavirus Classification Working Group. Next, we tried to establish a reverse genetics system for the avian RAV PO-13 strain to explore its pathogenicity to mammals. We constructed the reverse genetics system using T7 and polII promoter for synthesis of virus RNAs. Neither of the systems resulted in virus rescue. We, however, detected RAV specific replicon activity in the polII promoter system, indicating that the replication and transcription by the RAV polymerase could be induced artificially.

Research Products

• [Journal Article] Isolation and Characterization of a Novel Type of Rotavirus Species A in Sugar Gliders (Petaurus Breviceps). (2016)
  • Author(s): Okadera, K., Abe, M., Ito, N., Mitake, H., Okada, K., Nakagawa, K., Une, Y., Tsunemitsu, H. and Sugiyama, M.
  • Journal Title: J. Gen. Virol. Volume: 97 Issue: 5 Pages: 1158-1167
  • DOI: 10.1099/jgv.0.000433
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genome Sequences of Rotavirus A Strains Ty-1 and Ty-3, Isolated from Turkeys in Ireland in 1979. (2016)
  • Author(s): Fujii, Y., Mitake, H., Yamada, D., Nagai, M., Okadera, K., Ito, N., Okada, K., Nakagawa, K., Mizutani, T. and Sugiyama, M.
  • Journal Title: Genome Announc. Volume: 4 Issue: 1
  • DOI: 10.1128/genomea.01565-15
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Persistence of the rotavirus A genome in mesenteric lymph nodes of cattle raised on farms. (2015)
  • Author(s): Mitake, H., Ito, N., Okadera, K., Okada, K., Nakagawa, K., Tanaka, T., Katsuragi, K., Kasahara, K., Nihongi, T., Sakurai, S., Tsunemitsu, H. and Sugiyama, M.
  • Journal Title: J. Gen. Virol. Volume: 96 Issue: 9 Pages: 2708-2713
  • DOI: 10.1099/vir.0.000191
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Detection of avian-like rotavirus A VP4 from a calf in Japan (2015)
  • Author(s): Mitake, H., Ito, N., Okadera, K., Okada, K., Nakagawa, K., Tanaka, T., Katsuragi, K., Nihongi, T., Tsunemitsu, H., Sugiyama, M.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 2 Pages: 221-224
  • DOI: 10.1292/jvms.14-0379
  • NAID: 130004693916
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] アライグマ（Procyon lotor） から検出された鳥類ロタウイルスＡの全遺伝子の解析 (2016)
  • Author(s): 鳶田好将、三竹博道、岡寺康太、長井誠、伊藤直人、大松勤、水谷哲也、杉山誠
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 日本大学生物資源科学部
  • Year and Date: 2016-09-06
• [Presentation] ウシにおけるロタウイルスの存続様式 (2015)
  • Author(s): 三竹博道、岡寺康太、伊藤直人、岡田和真、中川賢人、田中知未、葛城粛仁、笠原香澄、二本木俊英、杉山 誠
  • Organizer: 第１５８回日本獣医学会学術集会
  • Place of Presentation: 北里大学獣医学部（十和田）
  • Year and Date: 2015-09-07
• [Presentation] 渡り鳥から検出された新型ロタウイルスAの遺伝学的解析 (2015)
  • Author(s): 藤井祐至、岡寺康太、三竹博道、伊藤直人、岡田和真、中川賢人、岡崎克則、迫田義博、高田礼人、小澤真、正谷達謄、杉山誠
  • Organizer: 第１５８回日本獣医学会学術集会
  • Place of Presentation: 北里大学獣医学部（十和田）
  • Year and Date: 2015-09-07
• [Presentation] 鳥類由来ロタウイルスAの遺伝学的解析による分子基盤の確立 (2014)
  • Author(s): 山田大悟、岡寺康太、三竹博道、伊藤直人、岡田和真、中川賢人、杉山 誠
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（札幌）
  • Year and Date: 2014-09-09 – 2014-09-12