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Molecular mechanisms for the acquisition of virulence by bat-borne reoviruses

Research Project

Project/Area Number 26292149
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Veterinary medical science
Research InstitutionOsaka University

Principal Investigator

Kobayashi Takeshi  大阪大学, 微生物病研究所, 准教授 (90324847)

Co-Investigator(Renkei-kenkyūsha) Kanai Yuta  大阪大学, 微生物病研究所, 助教 (80506501)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Keywordsレオウイルス / コウモリ
Outline of Final Research Achievements

Nelson Bay orthoreoviruses (NBVs) were recently isolated from humans with acute respiratory tract infections, suggesting that NBVs have evolved to propagate in humans. To better understand NBV replication and pathogenesis, we have developed a mouse model of NBV infection that mimics the disease in humans. Furthermore, using reverse genetics, we demonstrated that FAST, p17, and σC proteins encoded by S1 gene segment were not required for viral replication. Our results with mutant viruses demonstrated that fusion activity of FAST was critical for the enhancement of viral replication and that σC was dispensable for cell attachment in several cell lines, including L929 cells but not in A549 cells. Significant attenuation was observed in mice infected with FAST- and σC-deficient viruses compared to those infected with the wild-type, suggesting that FAST and σC play crucial roles in viral pathogenesis, and can provide new insights into the NBV biology.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (22 results)

All 2017 2016 2015 2014

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 5 results,  Acknowledgement Compliant: 2 results) Presentation (15 results) (of which Int'l Joint Research: 5 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Entirely plasmid-based reverse genetics system for rotaviruses2017

    • Author(s)
      Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M, Matsuura Y, Taniguchi K, Kobayashi T.
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 114 Issue: 9 Pages: 2349-2354

    • DOI

      10.1073/pnas.1618424114

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] African swine fever virus NP868R capping enzyme promotes reovirus rescue during reverse genetics by promoting reovirus protein expression, virion assembly, and RNA incorporation into infectious virions2017

    • Author(s)
      Eaton HE, Kobayashi T, Dermody TS, Johnston RN, Jais PH, Shmulevitz M.
    • Journal Title

      J Virol.

      Volume: 印刷中 Issue: 11

    • DOI

      10.1128/jvi.02416-16

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Reverse Genetics for Fusogenic Bat-Borne Orthoreovirus Associated with Acute Respiratory Tract Infections in Humans: Role of Outer Capsid Protein σC in Viral Replication and Pathogenesis2016

    • Author(s)
      Takahiro Kawagishi, Yuta Kanai, Hideki Tani, Masayuki Shimojima, Masayuki Saijo, Yoshiharu Matsuura, Takeshi Kobayashi
    • Journal Title

      PLoS Pathogens

      Volume: 12 Issue: 2 Pages: e1005455-e1005455

    • DOI

      10.1371/journal.ppat.1005455

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Rapid whole genome sequencing of Miyazaki-Bali/2007 Pteropine orthoreovirus by modified rolling circular amplification with adaptor ligation - next generation sequencing.2015

    • Author(s)
      Singh H, Yoshikawa T, Kobayashi T, Fukushi S, Tani H, Taniguchi S, Fukuma A, Yang M, Sugamata M, Shimojima M, Saijo M.
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 16517-16517

    • DOI

      10.1038/srep16517

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a Novel Dengue-1 Virus Replicon System Expressing Secretory <i>Gaussia</i> luciferase for Analysis of Viral Replication and Discovery of Antiviral Drugs2014

    • Author(s)
      Kato F., Kobayashi T., Tajima S., Takasaki T., Miura T., Igarashi T., and Hishiki T.
    • Journal Title

      Japanese Journal of Infectious Diseases

      Volume: 67 Issue: 3 Pages: 209-212

    • DOI

      10.7883/yoken.67.209

    • NAID

      130004757083

    • ISSN
      1344-6304, 1884-2836
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Imported case of acute respiratory tract infection associated with a member of species nelson bay orthoreovirus2014

    • Author(s)
      Atsushi Yamanaka
    • Journal Title

      PLoS One

      Volume: 9 Issue: 3 Pages: e92777-e92777

    • DOI

      10.1371/journal.pone.0092777

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Reverse genetics for fusogenic bat-borne orthoreovirus associated with acute respiratory tract infections in humans2017

    • Author(s)
      Takeshi Kobayashi
    • Organizer
      11th International Symposium of The Institute Network “Frontiers in Biomedical Sciences”
    • Place of Presentation
      徳島県
    • Year and Date
      2017-01-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In vivo live imaging of tumor by gene-modified mammalian orthoreovirus with2016

    • Author(s)
      Yuta Kanai, Takahiro Kawagishi, Yoshiharu Matsuura, Takeshi Kobayashi
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] Nelson Bay Orthoreovirus S1 Gene Segment Determines Strain-Specific2016

    • Author(s)
      Takahiro Kawagishi, Yuta Kanai, Hideki Tani, Masayuki Shimojima, Masayuki Saijo, Yoshiharu Matsuura, Takeshi Kobayashi
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] Roles of FAST proteins of fusogenic orthoreovirus in viral propagation and pathogenesis2016

    • Author(s)
      Yuta Kanai, Takahiro Kawagishi, Masayuki Shimojima, Masayuki Saijo, Yoshiharu Matsuura, Takeshi Kobayashi
    • Organizer
      第11回日中国際ウイルス学会
    • Place of Presentation
      香川県
    • Year and Date
      2016-07-01
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Expression and characterization of the Neison Bay orthoreovirus &micro;NS and σNS proteins2016

    • Author(s)
      Yong Gang Li, Yuta Kanai, Takahiro Kawagishi, Misa Onishi, Masayuki Shimojima, Masayuki Saijo, Yoshiharu Matsuura, Takeshi Kobayashi
    • Organizer
      第11回日中国際ウイルス学会
    • Place of Presentation
      香川県
    • Year and Date
      2016-07-01
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] BIOLOGICAL FUNCTIONS OF ORTHOREOVIRUS FAST PROTEINS IN VIRAL REPLICATION AND PATHOGENESIS2016

    • Author(s)
      Yuta Kanai, Takahiro Kawagishi, Masayuki Shimojima, Masayuki Saijo, Yoshiharu Matsuura, Takeshi kobayashi
    • Organizer
      American Society for Virology 2016
    • Place of Presentation
      Blacksburg・VirginiaTech
    • Year and Date
      2016-06-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] FUNCTIONAL ANALYSIS OF NELSON BAY ORTHOREOVIRUS CELL ATTACHMENT PROTEIN σC2016

    • Author(s)
      Takahiro Kawagishi, Yuta Kanai, Hideki Tani, Masayuki Shimojima, Masayuki Saijo, Yoshiharu Matsuura, Takeshi Kobayashi
    • Organizer
      American Society for Virology 2016
    • Place of Presentation
      Blacksburg・VirginiaTech
    • Year and Date
      2016-06-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional analysis of pteropine reovirus cell attachment protein sigmaC2015

    • Author(s)
      Takahiro Kawagishi, Yuta Kanai, Hideki Tani, Masayuki Shimojima, Masayuki Saijo, Yoshiharu Matsuura, Takeshi Kobayashi
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Biological functions of orthoreovirus FAST proteins in viral replication and pathognesis2015

    • Author(s)
      Yuta Kanai, Takahiro Kawagishi, Masayuki Shimojima, Masayuki Saijo, Yoshiharu Matsuura, Takeshi Kobayashi
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Oncolytic virotherapy using genetically engineered mammalian reovirus2015

    • Author(s)
      Yuta Kanai, Takahiro Kawagishi, Yoshiharu Matsuura, Takeshi Kobayashi
    • Organizer
      第21回日本遺伝子治療学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2015-07-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] 遺伝子改変オルソレオウイルスを用いた新規腫瘍溶解ベクターの開発2014

    • Author(s)
      金井 祐太、川岸 崇裕、松浦 善治、小林 剛
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 高病原性コウモリ由来レオウイルスのリバースジェネティクスの確立2014

    • Author(s)
      川岸 崇裕、金井 祐太、谷 英樹、下島 昌幸、西條 政幸、松浦 善治、小林 剛
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Fusogenic reovirusがコードするFAST蛋白質の機能解析2014

    • Author(s)
      金井 祐太、川岸 崇裕、下島 昌幸、西條 政幸、松浦 善治、小林 剛
    • Organizer
      第157回獣医学会学術集会
    • Place of Presentation
      札幌
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 高病原性コウモリ由来レオウイルスの遺伝子操作系の確立2014

    • Author(s)
      川岸 崇裕、金井 祐太、谷 英樹、下島 昌幸、西條 政幸、松浦 善治、小林 剛
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      札幌
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification and characterization of a new fusogenic orthoreovirus from a patient with acute respiratory infection2014

    • Author(s)
      Takahiro Kawagishi, Yuta Kanai, Hideki Tani, Masayuki Shimojima, Masayuki Saijo, Yoshiharu Matsuura, Takeshi Kobayashi
    • Organizer
      第10回日中国際ウイルス学会
    • Place of Presentation
      中国 長春
    • Year and Date
      2014-08-25 – 2014-08-27
    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] 人工組換えロタウイルスの作製方法2016

    • Inventor(s)
      小林剛、金井祐太
    • Industrial Property Rights Holder
      小林剛、金井祐太
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-09-27
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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