| Project/Area Number |
26292151
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Kyushu University |
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Principal Investigator |
Ono Etsuro 九州大学, 医学(系)研究科(研究院), 教授 (00160903)
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| Co-Investigator(Kenkyū-buntansha) |
河原崎 達雄 東海大学, 農学部, 教授 (70500247)
柴田 昌利 静岡県畜産技術研究所, その他部局等, その他 (70501443)
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| Co-Investigator(Renkei-kenkyūsha) |
IWAMORI NAOKI 九州大学, 大学院農学研究院, 准教授 (70647362)
TOMIOKA YUKIKO 鳥取大学, 農学部, 准教授 (50374674)
FUJIMOTO YOSHIKAZU 九州大学, 大学院医学研究院, 助教 (20613631)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Keywords | 豚繁殖・呼吸障害症候群 / ゲノム編集 / 抗病性動物 / PRRS |
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| Outline of Final Research Achievements |
Porcine reproductive and respiratory syndrome (PRRS) is the most economically important disease of swine worldwide. Vaccines have been unable to control the disease. It has been suggested that CD163 and/or Siglec 1 are the receptors for entry of porcine reproductive and respiratory syndrome virus (PRRSV) into cells. Thus, we hypothesized that pigs with defective CD163 and/or Siglec 1 would be resistant to PRRSV. Here, we have tried to generate PRRS-resistant pigs using CRISPR/Cas9 system by introducing mutations in the CD163 and Siglec 1 genes to secrete a soluble form of each molecule. Total 11 piglets were born from foster pigs transferred zygotes injected with each sgRNA and Cas9 and an adenine insertion was detected in the Siglec 1 gene in one of them. Unfortunately, the piglet exhibited a mosaic genotype. In addition, somatic cell nuclear transfer was performed using porcine fibroblasts possessing the CD163 gene edited by CRISPR/Cas9 system.
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