Identification of miRNA-mRNA network involved in regulation of the sex determination in lepidopteran insects
Project/Area Number |
26292172
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Insect science
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Research Institution | The University of Tokyo |
Principal Investigator |
Suzuki Masataka 東京大学, 新領域創成科学研究科, 准教授 (30360572)
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Co-Investigator(Renkei-kenkyūsha) |
AOKI Fugaku 東京大学, 大学院新領域創成科学研究科, 教授 (20175160)
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Research Collaborator |
SAKAI Hiroki
KASASHIMA Megumi
SEZUTSU Hideki
HATAKEYAMA Masatsugu
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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Keywords | 性決定 / 性分化 / microRNA / doublesex / カイコ / 発生分化 / 発現制御 / 昆虫 / miRNA / Ago1 / 発生 / dsx / let-7 / ショウジョウバエ |
Outline of Final Research Achievements |
To investigate whether microRNAs (miRNAs) are involved in sexual development of the silkworm Bombyx mori, we performed genome wide analysis for miRNAs that are highly expressed in the sex determination stage. The analysis identified that miR-2733i-3p is specifically expressed at the sex determination stage. Knockdown of miR-2733i-3p resulted in lower expression of the sex-determining gene Bmdsx in embryonic stage. in silico analysis predicted that Bmdsx is a possible target of miR-2733i-3p. Our validation analysis with reporter assays demonstrated that miR-2733i-3p binds to a target sequence in the Bmdsx 3’UTR and repress its translation. These results suggest that miR-2733i-3p may regulate sexual development of the silkworm by regulating the expression of Bmdsx.
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Report
(5 results)
Research Products
(49 results)
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[Journal Article] Transgenic Expression of the piRNA-Resistant Masculinizer Gene Induces Female-Specific Lethality and Partial Female-to-Male Sex Reversal in the Silkworm, Bombyx mori2016
Author(s)
Hiroki Sakai, Megumi Sumitani, Yasuhiko Chikami, Kensuke Yahata, Keiro Uchino, Takashi Kiuchi, Susumu Katsuma, Fugaku Aoki, Hideki Sezutsu, Masataka G. Suzuki
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Journal Title
PLOS Genetics
Volume: 12(8)
Issue: 8
Pages: e1006203-e1006203
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] The first murine zygotic transcription is promiscuous and uncoupled from splicing and 3' processing.2015
Author(s)
Abe, K., Yamamoto, R., Franke, V., Cao, M., Suzuki, Y., Suzuki, M.G., Vlahovicek, K., Svoboda, P., Schultz, R.M. and Aoki, F.
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Journal Title
EMBO J.
Volume: 34
Issue: 11
Pages: 1523-1537
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A single female-specific piRNA is the primary determiner of sex in the silkworm2014
Author(s)
Kiuchi T, Koga H, Kawamoto M, Shoji K, Sakai H, Arai Y, Ishihara G, Kawaoka S, Sugano S, Shimada T, Suzuki Y, Suzuki MG, Katsuma S.
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Journal Title
Nature
Volume: 509
Issue: 7502
Pages: 633-636
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Book] 生物の科学 遺伝 動植物の性別決定メカニズムの多様性2016
Author(s)
市原健介, 河野重行, 三浦郁夫, 尾形光昭, 赤木剛士, 風間裕介, 石井公太郎, 阿部知子, 鈴木雅京, 明正大純, 酒泉満, 黒岩麻里
Total Pages
432
Publisher
株式会社NTS
Related Report
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