Molecular mechanisms of rRNA degradation in NPV-infected insect cells
Project/Area Number |
26292173
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Insect science
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Research Institution | Nagoya University |
Principal Investigator |
IKEDA Motoko 名古屋大学, 生命農学研究科, 教授 (20262892)
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Co-Investigator(Kenkyū-buntansha) |
永峰 俊弘 国立研究開発法人理化学研究所, 佐甲細胞情報研究室, 専任研究員 (90237553)
山田 早人 名古屋大学, 生命農学研究科, 助教 (70778258)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
|
Keywords | 核多角体病ウイルス / 昆虫細胞 / 抗ウイルス応答 / リボソームRNA分解 / 全タンパク質合成停止 / 宿主域決定因子 / 昆虫培養細胞 / アポトーシス誘導 / RNA分解 / カイコ / RNA-seq解析 |
Outline of Final Research Achievements |
We previously showed that rRNA degradation occurs in Bombyx cells (BM-N cells) as an antiviral immune response during abortive infection with heterologous nucleopolyhedroviruses (NPVs). In the present study, we examined the induction mechanisms of rRNA degradation in Autographa californica NPV (AcMNPV) infected BM-N cells. We identified AcMNPV p143 (ac-p143) as an inducer of rRNA degradation. And then, we investigated the domain of Ac-P143 responsible for rRNA degradation, the cellular factors interacting with Ac-P143, and the signal transduction factors responsible for induction of rRNA degradation.
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Report
(5 results)
Research Products
(41 results)
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[Journal Article] rRNA degradation in Bombyx mori and Bombyx mandarina cells infected with heterologous nucleopolyhedroviruses.2016
Author(s)
Hamajima, R., Yasunaga-Aoki, C., Iwanaga, M., Imanishi, S., Kobayashi, J., Sasaki, K., Kusakabe, T., Lee J-M., Mon, H., Kobayashi, M. and Ikeda, M.
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Journal Title
J. Insect Biotechnol. Sericol.
Volume: 85
Pages: 73-77
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] hcf-1 gene of AcMNPV is an essential viral factor required for productive infection of HycuMNPV in Tn368 cells.2016
Author(s)
Tachibana, A., Hamajima, R., Tomizaki, M., Kondo, T., Nanba, Y., Kobayashi, M. and Ikeda, M.
Organizer
International Congress of Entomology 2016
Place of Presentation
フロリダ州,USA
Year and Date
2016-09-25
Related Report
Int'l Joint Research
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