Establishment of new satellite model system using Caenorhabditis sp.

• Project/Area Number: 26292178
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Insect science
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: Kanzaki Natsumi 国立研究開発法人森林総合研究所, きのこ・森林微生物研究領域, 主任研究員 (70435585)
• Co-Investigator(Kenkyū-buntansha): 菊地 泰生 宮崎大学, 医学部, 准教授 (20353659)
• Co-Investigator(Renkei-kenkyūsha): HASEGAWA Koichi 中部大学, 応用生物学部, 講師 (10609837)
• Research Collaborator: TANAKA Ryusei 宮崎大学, 医学部, 助教 (70723550)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
• Keywords: ゲノム / 胚発生 / 進化 / モデル生物 / イチジクコバチ / Caenorhabditis elegans / 生態 / 分類 / 線虫 / サテライトモデル / 生理生態

Outline of Final Research Achievements

Taxonomical, ecological, genomic and developmental features of an undescribed species, Caenorhabditis sp. 34 were examined to establish a new satellite model system. Regardless of its phylogenetic closeness to a model organism C. elegans, the basic biological features of undescribed species were clearly different from C. elegans, suggesting that the undescribed species can be an interesting satellite model system, especially for evolutionary biology.

Research Products

• [Int'l Joint Research] University of Oregon/University of Florida(米国)
• [Int'l Joint Research] Max Planck Institute(ドイツ)
• [Int'l Joint Research] University of Pretoria(南アフリカ)
• [Journal Article] Stomatal dimorphism of Neodiplogaster acaloleptae (Diplogastromorpha: Diplogastridae). (2016)
  • Author(s): Kanzaki, N.
  • Journal Title: PLoS ONE Volume: 11 Issue: 5 Pages: e0155715-e0155715
  • DOI: 10.1371/journal.pone.0155715
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Diplogasteroides nix n. sp. (Nematoda: Diplogastridae), a cryptic species related to D. andrassyi, isolated from Monochamus urussovii (Coleoptera: Cerambycidae) from Hokkaido, Japan, with remarks on the body surface structures (2016)
  • Author(s): Natsumi Kanzaki, Hironori Sakamoto and Noritoshi Maehara
  • Journal Title: Nematology Volume: 18 Issue: 7 Pages: 753-773
  • DOI: 10.1163/15685411-00002990
  • Peer Reviewed
• [Journal Article] Tylenchid entomoparasites isolated from Spondylis buprestoides (L.) and Asemum striatum (L) (Coleoptera: Cerambycidae). (2016)
  • Author(s): Kanzaki, N.、Giblin-Davis, R.M.、Gonzalez, R.、Trujillo, Y.、 Hajek, A.E.
  • Journal Title: Nematology Volume: 18 Issue: 7 Pages: 775-780
  • DOI: 10.1163/15685411-00002992
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Bursaphelenchus kesiyae n. sp (Nematoda: Aphelenchoididae), isolated from dead wood of Pinus kesiya Royle ex Gordon (Pinaceae) from Vietnam, with proposal of new subgroups in the B. fungivorus group (2016)
  • Author(s): Kanzaki, N., Aikawa, T., Maehara, N. & Thu, P.Q.
  • Journal Title: Nematology Volume: 18 Issue: 3 Pages: 133-146
  • DOI: 10.1163/15685411-00003051
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Recharacterization of three Parasitodiplogaster species based on morphological and molecular profiles. (2016)
  • Author(s): Kanzaki, N., Giblin-Davis, R.M., Ye, W., Herre, E.A. & Center, B.J.
  • Journal Title: Nematology Volume: 18 Issue: 4 Pages: 417-437
  • DOI: 10.1163/15685411-00002967
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Large-scale diversification without genetic isolation in nematode symbionts of figs. (2016)
  • Author(s): Susoy, V., Herrmann, M., Kanzaki, N., Kruger, M., Nguyen, C.N., Rödelsperger, C., Röseler, W., Weiler, C., Giblin-Davis, R.M., Ragsdale, E.J. & Sommer, R.J.
  • Journal Title: Science Advances Volume: 2 Issue: 1
  • DOI: 10.1126/sciadv.1501031
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Leptojacobus dorci n. gen., n. sp. (Nematoda: Diplogastridae), an associate of Dorcus stag beetles (Coleoptera: Lucanidae). (2014)
  • Author(s): Kanzaki, N.、Ragsdale, E.J.、Susoy, V.、Sommer, R.J.
  • Journal Title: Journal of Nematology Volume: 46 Pages: 50-59
  • Peer Reviewed / Open Access
• [Journal Article] Two new and two recharacterized species from a radiation of Pristionchus (Nematoda: Diplogastridae) in Europe (2014)
  • Author(s): Kanzaki, N.、Ragsdale, E.J.、Herrmann, M.、Sommer, R.J.
  • Journal Title: Journal of Nematology Volume: 46 Pages: 60-67
  • Peer Reviewed / Open Access
• [Journal Article] New Plant-Parasitic Nematode from the Mostly Mycophagous Genus Bursaphelenchus Discovered inside Figs in Japan (2014)
  • Author(s): Natsumi Kanzaki, Ryusei Tanaka, Robin M. Giblin-Davis, Kerrie A. Davies
  • Journal Title: PLOS ONE Volume: 9 Issue: 6 Pages: e99241-e99241
  • DOI: 10.1371/journal.pone.0099241
  • Peer Reviewed / Open Access
• [Journal Article] Parasitodiplogaster species associated with Pharmacosycea figs in Panama. (2014)
  • Author(s): Kanzaki, N.、Giblin-Davis, R.M.、Ye, W.、Herre, E.A.、Center, B.J.
  • Journal Title: Nematology Volume: 16 Issue: 5 Pages: 607-619
  • DOI: 10.1163/15685411-00002791
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Nomenclatural revision of Koerneria Meyl, 1960 (Diplogastridae) and resurrection of two other genera of Diplogastridae (Nematoda) (2014)
  • Author(s): Kanzaki, N.、Ragsdale, E.J.、Giblin-Davis, R.M.
  • Journal Title: Zookeys Volume: 442 Pages: 17-30
  • DOI: 10.3897/zookeys.442.7459
  • Peer Reviewed / Open Access
• [Journal Article] Teratodiplogaster variegatae n. sp. (Nematoda: Diplogastridae) isolated from the syconia of Ficus variegata Blume on Ishigaki Island, Okinawa, Japan (2014)
  • Author(s): Natsumi KANZAKI, Gavin C. WOODRUFF and Ryusei TANAKA
  • Journal Title: Nematology Volume: 16 Issue: 10 Pages: 1153-1166
  • DOI: 10.1163/15685411-00002843
  • Peer Reviewed
• [Journal Article] Molecular and morphological observations of Parasitodiplogaster sycophilon Poinar 1979 (Nematoda: Diplogastrina) associated with Ficus burkei in Africa. (2014)
  • Author(s): Woehr, M.、Greeff, J.M、Kanzaki, N.、Ye, W.、Giblin-Davis, R.M.
  • Journal Title: Nematology Volume: 16 Issue: 4 Pages: 453-462
  • DOI: 10.1163/15685411-00002777
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Levipalatum texanum n. gen., n. sp. (Nematoda: Diplogastridae), an androdioecious species from the south-eastern USA. (2014)
  • Author(s): Ragsdale, E.J.、Kanzaki, N.、Sommer, R.J.
  • Journal Title: Nematology Volume: 16 Issue: 6 Pages: 695-709
  • DOI: 10.1163/15685411-00002798
  • Peer Reviewed
• [Journal Article] Evidence of Hermaphroditism and Sex Ratio Distortion in the Fungal Feeding Nematode Bursaphelenchus okinawaensis (2014)
  • Author(s): Shinya R, Hasegawa K, Chen A, Kanzaki N, Sternberg PW
  • Journal Title: G3-Genes Genomes Genetics Volume: 4 Issue: 10 Pages: 1907-1917
  • DOI: 10.1534/g3.114.012385
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ficotylus laselvae n. sp. (Tylenchomorpha: Anguinidae) associated with Ficus colubrinae in Costa Rica (2014)
  • Author(s): Giblin-Davis, R.M.、Kanzaki, N.、Davies, K.A.、Ye, W.、Zeng, Y.、Center, B.J.、Esquivel, A.、Powers, T.O.
  • Journal Title: Nematology Volume: 16 Issue: 10 Pages: 1139-1151
  • DOI: 10.1163/15685411-00002839
  • Peer Reviewed
• [Journal Article] Distribution and evolution of glycoside hydrolase family 45 cellulases in nematodes and fungi (2014)
  • Author(s): Juan E Palomares-Rius, Yuuri Hirooka, Isheng J Tsai, Hayato Masuya, Akina Hino, Natsumi Kanzaki, John T Jones, Taisei Kikuchi
  • Journal Title: BMC evolutionary biology Volume: 14 Issue: 1 Pages: 69-69
  • DOI: 10.1186/1471-2148-14-69
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] High quality genome sequence of Caenorhabditis sp. 34, the sister species of C. elegans (2016)
  • Author(s): Taisei Kikuchi, Isheng Jason Tsai, Ryusei Tanaka, Gavin Woodruff, Asako Sugimoto, Natsumi Kanzaki
  • Organizer: Evolutionary Biology of Caenorhabditis and Other Nematodes
  • Place of Presentation: Cold Spring Harbor, NY
  • Year and Date: 2016-03-30
  • Int'l Joint Research
• [Presentation] Genetic and molecular tools for Caenorhabditis sp. 34, a sister species of C. elegans (2016)
  • Author(s): Asako Sugimoto, Satoshi Namai, Kenji Tsuyama, Ryohei Kumagai, Takuya Shimura, Natsumi Kanzaki, and Taisei Kikuchi
  • Organizer: Evolutionary Biology of Caenorhabditis and Other Nematodes
  • Place of Presentation: Cold Spring Harbor, NY
  • Year and Date: 2016-03-30
  • Int'l Joint Research
• [Presentation] Morphology and Ecology of fig fruits nematode Caenorhabditis sp. 34 (2016)
  • Author(s): Ryusei Tanaka, Asako Sugimoto, Natsumi Kanzaki, Taisei Kikuchi
  • Organizer: Evolutionary Biology of Caenorhabditis and Other Nematodes
  • Place of Presentation: Cold Spring Harbor, NY
  • Year and Date: 2016-03-30
  • Int'l Joint Research
• [Presentation] Dramatic evolution of body length due to post-embryonic changes in cell size in a newly discovered close relative of C. elegans (2016)
  • Author(s): Gavin Woodruff, Taisei Kikuchi, Patric Phillips, Natsumi Kanzaki
  • Organizer: Evolutionary Biology of Caenorhabditis and Other Nematodes
  • Place of Presentation: Cold Spring Harbor, NY
  • Year and Date: 2016-03-30