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Comprehensive analysis of maintenance mechanism of forest soil microbial ecosystem by thermal disturbance

Research Project

Project/Area Number 26292180
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Environmental agriculture(including landscape science)
Research InstitutionHokkaido University

Principal Investigator

Kasahara Yasuhiro  北海道大学, 低温科学研究所, 准教授 (20273849)

Co-Investigator(Kenkyū-buntansha) 小椋 義俊  九州大学, 医学研究院, 准教授 (40363585)
Research Collaborator SATO Tadashi  
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywords微生物生態 / メタゲノム / 森林土壌 / 微生物群集構造 / 撹乱 / 群集構造解析 / 土壌
Outline of Final Research Achievements

We aimed to clarify the maintenance mechanism of microbial ecosystem of forest soil with short-term high-temperature and long-term low-temperature disturbances with resistance, resilience and function redundancy as stability index.
<Short-term/high-temperature disturbance> In the pyrotechnic field, soil microbial community structure analysis was performed before the burning and one day to three years after the burning. Disappearance of diversity that single species prefers immediately after burning is observed, and in the third year it is getting close to composition before burning. The resilience was shown to be short-term. <Long-term/low temperature disturbance> In the warming field with the ground temperature of +5°C, no significant change in microbial community composition was observed compared with the untreated field despite soil warming for 10 years. It showed the robustness of the soil microbial community.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (15 results)

All 2018 2017 2016 2015 2014

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] Understory Dwarf Bamboo Affects Microbial Community Structures and Soil Properties in a <i>Betula ermanii</i> Forest in Northern Japan2017

    • Author(s)
      Kong, B., L. Chen, Y. Kasahara, A. Sumida, K. Ono, J. Wild, A. Nagatake, R. Hatano, T. Hara
    • Journal Title

      Microbes and Environments

      Volume: 32 Issue: 2 Pages: 103-111

    • DOI

      10.1264/jsme2.ME16154

    • NAID

      130006789870

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteome analysis of Pseudomonas putida F1 genes induced in soil environments.2016

    • Author(s)
      Morimoto H., Kadoya R, Takahashi K, Kasahara Y.
    • Journal Title

      Environmental Microbiology Reports

      Volume: 8 Issue: 5 Pages: 825-832

    • DOI

      10.1111/1758-2229.12445

    • NAID

      120006220031

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 土壌プロテオミクスから考える微生物生態系機能2016

    • Author(s)
      笠原康裕
    • Journal Title

      土と微生物

      Volume: 70 Pages: 41-44

    • NAID

      130005251350

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] An essential enzyme for phospholipid synthesis has a crucial role in Bacillus subtilis cell division2014

    • Author(s)
      Hiraku Takada, Sanae Fukushima-Tanaka, Masato Morita, Yasuhiro Kasahara, Satoru Watanabe, Taku Chibazakura, and Hirofumi Yoshikawa
    • Journal Title

      Mol Microbiol

      Volume: 91(2) Issue: 2 Pages: 242-255

    • DOI

      10.1111/mmi.12457

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metaproteomic Identification of Diazotrophic Methanotrophs, and their Tissue Localization in Field-grown Rice Roots2014

    • Author(s)
      Bao, Z., T. Okubo, K. Kubota, Y. Kasahara, H. Tsurumaru, M. Anda, S. Ikeda, K. Minamisawa
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 80 Issue: 16 Pages: 5043-5052

    • DOI

      10.1128/aem.00969-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] 環境オミックス解析によるソルガム根の窒素固定 Bradyrhizobium 属細菌の同定2018

    • Author(s)
      原新太郎、森川峻志、新井沙和、笠原康裕、小柴柴太、山崎清志、藤原徹、徳永毅、南澤究
    • Organizer
      第12回日本ゲノム微生物学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ゲノム科学から見えてくる微生物によるヒ素循環2017

    • Author(s)
      天知誠吾、土屋達哉、笠原康裕、濱村奈津子
    • Organizer
      JpGU-AGU Joint Meeting 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] メソルガム根の窒素固定活性とその原因窒素固定細菌の探索2017

    • Author(s)
      森川峻志、原新太郎、笠原康裕、小柴太一、山崎清志、藤原 徹、徳永 毅、南澤 究
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 土壌プロテオミクスから考える微生物生態系機能2016

    • Author(s)
      笠原康裕
    • Organizer
      日本土壌微生物学会2016年度大会
    • Place of Presentation
      岐阜大学(岐阜県・岐阜市)
    • Year and Date
      2016-06-11
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] プロテオーム解析を用いたPseudomonas putida F1株の土壌特異的発現遺伝子の同定と特性解析2016

    • Author(s)
      森本 一、門屋亨介、高橋一弘、笠原康裕
    • Organizer
      第10回日本ゲノム微生物学会年会
    • Place of Presentation
      東京工業大(東京都目黒区)
    • Year and Date
      2016-03-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] Proteome analysis of bacterial genes specifically expressed in soil.2015

    • Author(s)
      Yasuhiro Kasahara
    • Organizer
      ILTS International Symposium
    • Place of Presentation
      Hokkaido University(北海道札幌市)
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification and characterization of the soil-specific expression genes in Pseudomonas putida.2015

    • Author(s)
      Hajime Morimoto, Ryosuke Kadoya, Kazuhiro Takahashi and Yasuhiro Kasahara
    • Organizer
      第30回日本微生物生態学会
    • Place of Presentation
      土浦亀城プラザ(茨城県土浦市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] N2-fixing methanotroph as non-leguminous rhizobia in paddy rice roots.2014

    • Author(s)
      Kiwamu Minasamiwa, Zhihua Bao, Seishi Ikeda, Takashi Okubo, Haruko Imaizumi-Anraku, Kengo Kubota, Yasuhiro Kasahara, Dongyan Liu, Susumu Asakawa
    • Organizer
      14th International Symposium on Nitrogen Fixation with Non-Legumes
    • Place of Presentation
      Chengdu, China
    • Year and Date
      2014-10-30
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 水稲根における窒素固定メタン酸化細菌のメタプロテオーム同定と組織局在性2014

    • Author(s)
      包智華、大久保卓、久保田健吾、笠原康裕、鶴丸博人、按田瑞恵、池田成志、南澤 究
    • Organizer
      日本土壌肥料学会2014年度大会
    • Place of Presentation
      東京農工大(東京都府中市)
    • Year and Date
      2014-09-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] No nitrogen fertilization changes rice root microbiome abundant in diazotrophic methanotrophs through a plant symbiosis gene.2014

    • Author(s)
      Kiwamu Minamisawa, Seishi Ikeda, Zhihua Bao, Takashi Okubo, Aya Watanabe, Kazuhiro Sasaki, Haruko Imaizumi-Anraku, Takeshi Tokida, Kengo Kubota, Yasuhiro Kasahara, Dongyan Liu, Takeshi Watanabe, Jun Murase, Susumu Asakawa, Mizue Anda, Hirohito Tsurumaru, Ryo Shinoda, Tadashi Sato
    • Organizer
      15th International Symposium on Microbial Ecology
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2014-08-24
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research

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Published: 2014-04-04   Modified: 2019-03-29  

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